Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   107742
Name   oriT_pX2-15 in_silico
Organism   Salmonella enterica strain X2-15
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MW025962 (44465..44553 [-], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_pX2-15
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGTAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTACACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   5200 GenBank   WP_001493820
Name   nikB_MZV74_RS00290_pX2-15 insolico UniProt ID   A0A229B7S1
Length   900 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 900 a.a.        Molecular weight: 104037.65 Da        Isoelectric Point: 7.3321

>WP_001493820.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELNLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVDFYGVTCFHNCTSLETAAADMEYIAQQAHYAKDNTDPVFHYILSWQAHESPRPEQIYDSVR
HTLKSLGLGEHQYVSAVHTDTDNLHVHVAVNRVHPVTGYLNCLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVDAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHCTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLNGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDKSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRIVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
YNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKLWEPPSPMR

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB A0A229B7S1


Auxiliary protein

ID   2367 GenBank   WP_001283947
Name   WP_001283947_pX2-15 insolico UniProt ID   A0A142CMC2
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12613.57 Da        Isoelectric Point: 10.7463

>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A142CMC2


T4CP

ID   5447 GenBank   WP_001289272
Name   trbC_MZV74_RS00300_pX2-15 insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86924.04 Da        Isoelectric Point: 6.6402

>WP_001289272.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPIVINRYISVDAPRLDRLRRLVPRTAQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETNEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKNNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 50911..89022

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MZV74_RS00295 47736..48554 + 819 WP_000106196 DUF2971 domain-containing protein -
MZV74_RS00300 48627..50918 - 2292 WP_001289272 F-type conjugative transfer protein TrbC -
MZV74_RS00305 50911..51981 - 1071 WP_001535705 IncI1-type conjugal transfer protein TrbB trbB
MZV74_RS00310 52000..53208 - 1209 WP_001535704 IncI1-type conjugal transfer protein TrbA trbA
MZV74_RS00315 53500..53652 + 153 WP_001331364 Hok/Gef family protein -
MZV74_RS00320 53724..53975 - 252 WP_001535703 hypothetical protein -
MZV74_RS00575 54476..54571 + 96 WP_000609148 DinQ-like type I toxin DqlB -
MZV74_RS00330 54636..54920 - 285 WP_024129996 hypothetical protein -
MZV74_RS00335 55021..55230 - 210 WP_001140545 hemolysin expression modulator Hha -
MZV74_RS00340 55328..55942 - 615 WP_000578649 plasmid IncI1-type surface exclusion protein ExcA -
MZV74_RS00345 56018..58186 - 2169 WP_000698360 IncI1-type conjugal transfer membrane protein TraY traY
MZV74_RS00350 58283..58867 - 585 WP_001037987 IncI1-type conjugal transfer protein TraX -
MZV74_RS00355 58896..60098 - 1203 WP_001189156 IncI1-type conjugal transfer protein TraW traW
MZV74_RS00360 60065..60679 - 615 WP_000337400 IncI1-type conjugal transfer protein TraV traV
MZV74_RS00365 60679..63723 - 3045 WP_001024780 IncI1-type conjugal transfer protein TraU traU
MZV74_RS00370 63813..64613 - 801 WP_001164788 IncI1-type conjugal transfer protein TraT traT
MZV74_RS00375 64597..64785 - 189 WP_001277255 putative conjugal transfer protein TraS -
MZV74_RS00380 64849..65253 - 405 WP_000086960 IncI1-type conjugal transfer protein TraR traR
MZV74_RS00385 65304..65831 - 528 WP_001055569 conjugal transfer protein TraQ traQ
MZV74_RS00390 65831..66535 - 705 WP_000801920 IncI1-type conjugal transfer protein TraP traP
MZV74_RS00395 66535..67824 - 1290 WP_001535701 conjugal transfer protein TraO traO
MZV74_RS00400 67827..68810 - 984 WP_001191878 IncI1-type conjugal transfer protein TraN traN
MZV74_RS00405 68821..69513 - 693 WP_000138552 DotI/IcmL family type IV secretion protein traM
MZV74_RS00410 69510..69857 - 348 WP_001055900 conjugal transfer protein traL
MZV74_RS00415 69875..73642 - 3768 WP_001141542 LPD7 domain-containing protein -
MZV74_RS00420 73732..74283 - 552 WP_000014584 phospholipase D family protein -
MZV74_RS00425 74298..74588 - 291 WP_001372180 hypothetical protein traK
MZV74_RS00430 74585..75733 - 1149 WP_001024972 plasmid transfer ATPase TraJ virB11
MZV74_RS00435 75730..76548 - 819 WP_000646098 IncI1-type conjugal transfer lipoprotein TraI traI
MZV74_RS00440 76545..77003 - 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
MZV74_RS00445 77398..77982 - 585 WP_000977522 histidine phosphatase family protein -
MZV74_RS00450 78042..79244 - 1203 WP_000976351 conjugal transfer protein TraF -
MZV74_RS00455 79329..80153 - 825 WP_001238939 conjugal transfer protein TraE traE
MZV74_RS00460 80304..81458 - 1155 WP_001139957 site-specific integrase -
MZV74_RS00465 81442..81768 + 327 WP_001213803 hypothetical protein -
MZV74_RS00470 82063..82320 - 258 WP_001302706 hypothetical protein -
MZV74_RS00475 82633..82788 - 156 WP_001302705 hypothetical protein -
MZV74_RS00480 82979..83227 + 249 WP_001349157 hypothetical protein -
MZV74_RS00485 83224..84516 - 1293 WP_001417545 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
MZV74_RS00490 84516..85172 - 657 WP_001193553 prepilin peptidase -
MZV74_RS00495 85157..85717 - 561 WP_000014116 lytic transglycosylase domain-containing protein virB1
MZV74_RS00500 85727..86341 - 615 WP_000959785 type 4 pilus major pilin -
MZV74_RS00505 86359..87456 - 1098 WP_001208805 type II secretion system F family protein -
MZV74_RS00510 87469..89022 - 1554 WP_000362202 ATPase, T2SS/T4P/T4SS family virB11
MZV74_RS00515 89033..89485 - 453 WP_001247336 type IV pilus biogenesis protein PilP -
MZV74_RS00520 89472..90767 - 1296 WP_000752774 type 4b pilus protein PilO2 -
MZV74_RS00525 90760..92442 - 1683 WP_000748143 PilN family type IVB pilus formation outer membrane protein -
MZV74_RS00530 92456..92893 - 438 WP_000539807 type IV pilus biogenesis protein PilM -
MZV74_RS00535 92893..93960 - 1068 WP_000742600 type IV pilus biogenesis lipoprotein PilL -

Host bacterium

ID   8177 GenBank   NZ_MW025962
Plasmid name   pX2-15 Incompatibility group   IncI1
Plasmid size   99538 bp Coordinate of oriT [Strand]   44465..44553 [-]
Host baterium   Salmonella enterica strain X2-15

Cargo genes

Drug resistance gene   fosA3, blaCTX-M-14
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -