Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 107663
• Name: oriT_OyaliB|p2
• Organism: Rhizobium leguminosarum strain OyaliB
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP071401 (471558..471586 [+], 29 nt)
• oriT length: 29 nt
• IRs (inverted repeats): 14..19, 24..29 (CGTCGC..GCGACG)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 29 nt

>oriT_OyaliB|p2
AGGGCGCAATATACGTCGCTGTCGCGACG

oriT secondary structure

Predicted by RNAfold.

Relaxase

traA_J0664_RS31740_OyaliB|p2

• ID: 5152
• GenBank: WP_207159578
• Name: traA_J0664_RS31740_OyaliB|p2
• UniProt ID: _
• Length: 1538 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 1538 a.a.
• Molecular weight: 170522.68 Da
• Isoelectric Point: 7.3478

>WP_207159578.1 Ti-type conjugative transfer relaxase TraA [Rhizobium leguminosarum]
MAIMFVRAQVISRGVGRSIVSAAAYRHRARMMDEQAGTSFSYRGGRAELVHEELALPTQTPEWLRTAIDG
RTVAAASEALWNAVDAFEKRADAQLARELIIALPEELTRAENIALVREFVRDNLTSKGMVADWVYHDKDG
NPHIHLMTALRPLTQEGFGRKKVAVTGEDGAPLRVVTPDRPKGRIVYKLWAGDKETMKAWKIAWAETANR
HLALAGHEIRIDGRSYAEQGLDGIAQRHLGPEKAALSRRGAELYFAPADLARRQEMADRLLADPALLLKQ
LGNERSTFDERDIAKALHRTVDDPADFANIRARLMASDDLIMLKPQQVDAETGKASEPAVFTTREILRIE
YDMARSAQVLSERRGFAVSDRAVAAAIRNVETRDPERSFRLDAEQVDAVRHVTGDSGIAAVVGLAGAGKS
TLLAAARVAWESGGRRVIGAALAGKAAEGLEDSSGIRSRTLASWELIWANGHETLHRGDVLVIDEAGMVS
SQQMARVLKLMEQAGAKVVLVGDAMQLQPIQAGAAFRAITERIGFAELAGVRRQREEWAREASRLFARGE
IEKGLDAYAGHGHLVEAETRAETIDRIVADWTEARKQAIRNSTVVADDGRLRGDELLVLAHTNQDVKRLN
ETLRSVMSDAGALGENRAFQTERGGREFAAGDRIIFLANARFLEPRAPRLGPQYVKNGMLGTVVSTGDKR
GDALLTVRLDNGRDVVISEDSYRNVDHGYAATIHKSQGATVERTFVLATGMMDQHLTYVSMTRHRDRADL
YAAKEDFEARPEWGRKPRVDHAAGVTGELVETGQAKFRPNDEDADDSPYADVKTDDGSVHRLWGVSLPKA
LEEGGVSEGDTVTLRKDGVERVKVQVPIVDEQTGQKRFEEREVDRNVWAAKRIETAEARLERIERESHRP
ELFKQLVERLSRSGAKTSTLDFESESGYQAHADDFARRRGIDTLSQAAAGMEEEVSRRWAWIAEKREQVA
ELWERASMALGFAIERERRVSYNEARIETQPVSDADAGRARHLIVPTTVFARTVDEDARLAQRSSPAWME
REAILRPLLEKIYRDPDAALVVLNALSSDASIEPRRLVDDLAATPDRLGRLRGSDLIVDGRAARDERNAA
ITALTELLPLARAHATEFRRNAERFDLREQQRRAHMSLPIPALSRQAMARLVEVEAVRSQGGDDAYKTAF
AFATEDRSLVQEVKAVSEALTARFGWSAFTSKADAIAERNVVERMPEDLTSTRREKLTRLFEVVKRFAEE
QHLVERRDRSKIVAGASVDPEKENVPMLAAVTEFKTPIDEEARSRVLSDPLYRQQRAALAAVAGAIWRDP
AGAVGKIEELLQKGFAADRIAAAVTNDPAAYGALSGSDRLMDRMLATGRERKEAVQAVPEAAARLRALGS
AYVKVLDGERQAIAEERRRMAVAIPGLSKPAEDVLMRLTAEAKNNGRKRNASAVSLDPTIRQEFAAVSTA
LDERFGRNAILRDEKDLINRVPPVQRRAFEAMQERLRILQTTVRMESSDKIVSERRQRAVGRGRGIGF

Protein domains

Predicted by InterproScan.

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (1305-1399)

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (1056-1127)

• AAA domain (AAA_30) (391-578)

• MobA/MobL family (MobA_MobL) (17-249)

Protein structure

No available structure.

Host bacterium

• ID: 8098
• GenBank: NZ_CP071401
• Plasmid name: OyaliB|p2
• Incompatibility group: -
• Plasmid size: 538428 bp
• Coordinate of oriT [Strand]: 471558..471586 [+]
• Host baterium: Rhizobium leguminosarum strain OyaliB

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: actP
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -