Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   107662
Name   oriT_OyaliB|p1 in_silico
Organism   Rhizobium leguminosarum strain OyaliB
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP071400 (95853..95881 [-], 29 nt)
oriT length   29 nt
IRs (inverted repeats)      14..19, 24..29  (CGTCGC..GCGACG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 29 nt

>oriT_OyaliB|p1
AGGGCGCAATATACGTCGCTGTCGCGACG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   5151 GenBank   WP_207159192
Name   traA_J0664_RS24360_OyaliB|p1 insolico UniProt ID   _
Length   1539 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1539 a.a.        Molecular weight: 170671.04 Da        Isoelectric Point: 7.5064

>WP_207159192.1 Ti-type conjugative transfer relaxase TraA [Rhizobium leguminosarum]
MAIMFVRAQVISRGVGRSIVSAAAYRHRARMMDEQAGTSFSYRGGRAELVHEELALPTQTPEWLRTAIDG
RTVAAASEALWNAVDAFEKRADAQLARELIIALPEELTRAENIALVREFVRDNLTSKGMVADWVYHDKDG
NPHIHLMTALRPLTQEGFGRKKVAVTGEDGAPLRVVTPDRPKGRIVYKLWTGDKETMKAWKIAWAETANR
HLALAGHEIRIDGRSYAEQGLDGIAQRHLGPEKAALSRRGAELYFAPADLARRQEMADRLLADPALLLKQ
LGNERSTFDERDIAKALHRTVDDPLDFANIRARLMASDELVMLKPQQLDAETGKASEPAVFTTREILRIE
YDMARSAQVLSERCGFAVSDRVVAAAIRHVETQDLEKPFKLDAEQVDAVRHITGDSGIAAVVGLAGAGKS
TLLAAARVAWESGGRRVIGAALAGKAAEGLEDSSGIRSRTLAAWELTWANGRDTLHRGDVLVIDEAGMVS
SQQMARVLKLVEQAGAKVVLVGDAMQLQPIQAGAAFRAITERIGFAELAGVRRQREEWAREASRLFARGE
IEKGLDAYAGHGHLVEAETRAETIDRIVADWTEARKQAIRNSTVVADDGRLRGDELLVLAHTNQDVKRLN
EALRSVMSGEGALGEGRSFQTERGAREFAAGDRIIFLENARFLEPRAPRLGPQYVKNGMLGTVVSTGDKR
GEVLLSVRLDNGRDVVLSEDSYRNVDHGYAATIHKSQGATVERTFVLATGMMDQHLTYVSMTRHRDRADL
YAAKEDFEARPEWGRKPRVDHAAGVTGELVETGQAKFRPNDEDADDSPYADVKTDDGSVHRLWGVSLPKA
LEEGGVSEGDTVTLRKDGVEKVKVQVPIVDEQTGQKRFEEREVDRNVWTAKRIETADARLERIERESHRP
ELFKQLVLRLSRSGAKTTTLDFESEAGYQAHADDFARRRGIDTLSQAAAGMEEEVSRRWAWIAEKREQVA
ELWERASMALGFAIERERRVSYNEARIETQPVSDADAGRARHLIVPTTVFARTVDEDARLAQRSSPAWME
REAILRPLLEKIYRDPDAALVVLNALSSDASIEPRRLVDDLAATPDRLGRLRGSDLIVDGRAARDERSIA
TAALLELIPLARAHATEFRRNAERFGFREQQRRAHMSLPIPALSRQAMARLVEIEAVRDWGGDDAYKTAF
ALATEDRSLVQEVKAVSEALTARFGWSAFTSKADAIAERNIAGRLPEDLASTRREKLVGLFEAVKRFADE
QHLAERRDRSKIVAGASVERKNEHVAVLPMLAAVTEFKTPVDEEARSRVLSAPLYRQQRAVLAEAARAIW
RDPAGAVGKIEELLQKGFAADRIAAAVTNDPAAYGALRGSDRLMDRMLASGRERKDAVQAVSEAAARLRA
LGSTYVNVLDGERQAIAEERRRMTVAIPGLSKAAEEALMRLTAEAKNNGRKLSAPAASLDPDIRREFEAV
SRALDERFGRDAILRQAKDRINRVPPPHRTAFEAMQDRLKILQQAVRMESSDRIISERRQRTANRGREI

  Protein domains


Predicted by InterproScan.

BID domain of Bartonella effector protein (Bep) (Bep_C_terminal)

(1308-1401)

BID domain of Bartonella effector protein (Bep) (Bep_C_terminal)

(1056-1124)

AAA domain (AAA_30)

(391-578)

MobA/MobL family (MobA_MobL)

(17-249)


  Protein structure



No available structure.




T4CP

ID   5391 GenBank   WP_168342631
Name   traG_J0664_RS24375_OyaliB|p1 insolico UniProt ID   _
Length   639 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 639 a.a.        Molecular weight: 70450.23 Da        Isoelectric Point: 9.1439

>WP_168342631.1 MULTISPECIES: Ti-type conjugative transfer system protein TraG [Rhizobium]
MALKCRLHPGLLLVLVPVAVTAMAVYITGWRWSALAHGLSGKSEYWFMRAAPVPALLFGPLTGLLTVWAL
PLHRRRSIAVASLLCFLVVSSFYALREYGRLAPSVESGTVSWDRALSYLDFVAVIGALAGFMAVAVAARI
SVVVPEQVKRAKGGTFGDADWLSMSAAAKLFPPDGEIVVGERYRVDKDIVHELPFDPNDRNTWGQGGKAP
LLTYSQDFDSTHMLFFAGSGGYKTTSNVLPTALRYAGPLICLDPSTEVAPMVLEHRTRALGREVMVLDPT
NPIVGFNVLDGIEASKQKEEDIVGIAHMLLSESLRFESSTGSYFQNQAHNLLTGLLAHVMLSPEYAGRRN
LRSLRQIVSEPEPSVLAMLRDIQEHSESAFIRETLGVFTNMTEQTFSGVYSTASKDTQWLSLDSYAALVC
GNTFRSSDIIAGGTDVFLNIPASILRSYPGIGRVIIGSLINAMVQADGRFSRRALFMLDEVDLLGYIRVL
EEARDRGRKYGISMMLLYQSVGQLERHFGKDGATSWIDGCAFVSYAAIKALDTARNVSAQCGEMTVEVQG
RSRNIGWDTKRGGTRKSESVNFQRRPLIMPHEITQSMRKDEQIIIVQGHRPIRCGRAIYFRRKDMNAAAK
ANRFVKPVP

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(161-634)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 650881..660488

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J0664_RS36305 646799..646984 + 186 WP_246767367 hypothetical protein -
J0664_RS36310 646971..647168 + 198 WP_246767368 hypothetical protein -
J0664_RS26800 (J0664_26800) 647477..648086 + 610 Protein_585 integrase -
J0664_RS26805 (J0664_26805) 648161..648595 - 435 WP_207158858 type II toxin-antitoxin system VapC family toxin -
J0664_RS26810 (J0664_26810) 648595..648837 - 243 WP_046601781 type II toxin-antitoxin system Phd/YefM family antitoxin -
J0664_RS26815 (J0664_26815) 649271..650179 + 909 WP_207158859 nucleotidyltransferase and HEPN domain-containing protein -
J0664_RS26820 (J0664_26820) 650510..650725 + 216 WP_207158860 DUF3072 domain-containing protein -
J0664_RS26825 (J0664_26825) 650881..651921 - 1041 WP_207158861 P-type DNA transfer ATPase VirB11 virB11
J0664_RS26830 (J0664_26830) 651929..653101 - 1173 WP_207158862 type IV secretion system protein VirB10 virB10
J0664_RS26835 (J0664_26835) 653111..653968 - 858 WP_207158863 P-type conjugative transfer protein VirB9 virB9
J0664_RS26840 (J0664_26840) 653965..654636 - 672 WP_207158864 virB8 family protein virB8
J0664_RS26845 (J0664_26845) 654638..654922 - 285 WP_207158865 hypothetical protein -
J0664_RS26850 (J0664_26850) 654974..655894 - 921 WP_207158866 type IV secretion system protein virB6
J0664_RS26855 (J0664_26855) 655898..656131 - 234 WP_207158867 EexN family lipoprotein -
J0664_RS26860 (J0664_26860) 656128..656565 - 438 WP_348651639 P-type DNA transfer protein VirB5 -
J0664_RS36695 656474..656827 - 354 WP_348651640 type IV secretion system protein -
J0664_RS26865 (J0664_26865) 656827..659193 - 2367 WP_207158868 VirB4 family type IV secretion system protein virb4
J0664_RS26870 (J0664_26870) 659186..659524 - 339 WP_062941069 type IV secretion system protein VirB3 virB3
J0664_RS26875 (J0664_26875) 659530..659829 - 300 WP_065282894 TrbC/VirB2 family protein virB2
J0664_RS26880 (J0664_26880) 659826..660488 - 663 WP_207158869 lytic transglycosylase domain-containing protein virB1
J0664_RS26885 (J0664_26885) 660492..661031 - 540 WP_207158870 hypothetical protein -
J0664_RS26890 (J0664_26890) 661123..661476 + 354 WP_168342759 MarR family transcriptional regulator -
J0664_RS26895 (J0664_26895) 662142..663137 + 996 WP_207159451 glycosyltransferase -

Host bacterium

ID   8097 GenBank   NZ_CP071400
Plasmid name   OyaliB|p1 Incompatibility group   -
Plasmid size   1230906 bp Coordinate of oriT [Strand]   95853..95881 [-]
Host baterium   Rhizobium leguminosarum strain OyaliB

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   fixB, fixA
Anti-CRISPR   AcrIIA7