Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   107654
Name   oriT_ZX09|unnamed1 in_silico
Organism   Rhizobium sp. ZX09
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP031365 (35796..35848 [+], 53 nt)
oriT length   53 nt
IRs (inverted repeats)      23..28, 33..38  (CGTCGC..GCGACG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 53 nt

>oriT_ZX09|unnamed1
GGATCCGAAGGGCGCAATTATACGTCGCTGATGCGACGCGTTGCTTGTGGGCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   5144 GenBank   WP_207591868
Name   traA_BTN45_RS24690_ZX09|unnamed1 insolico UniProt ID   _
Length   1193 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1193 a.a.        Molecular weight: 132439.08 Da        Isoelectric Point: 7.8546

>WP_207591868.1 Ti-type conjugative transfer relaxase TraA [Rhizobium sp. ZX09]
MAVPHFSVSIVARGSGRSAVLSAAYRHCARMDYEREARTIDYTRKQGLLHEEFVIPPDAPEWLQSMIADR
SVSGASEAFWNKVEEFEKRSDAQLAKDITIALPLELSAEQNIELVRDFVARHITAQGMVVDWVFHDAPGN
PHIHLMTTLRPLTEDGFGPKKVAVTGPDSQPLRNDSGKIVYELWAGGLENFNGFRDGWFDCLNRHLALAG
LDIRVDGRSFEKQGIELTPTIHLGVGTKAIERKAEGVEEKAALERLELQEGRRAENARRIQRNPEIVLDL
ITREKSVFDERDVAKILHRYIDDAGLFQSLMARILQSPETLRLDRERVNFASGARTSVKYTTRELIRLEA
EMANRAIWLSQRSSHGVRKAVLDTTFARHDRLSDEQKTAIEHVAGDAGIAAVIGRAGAGKTTMMKAAREV
WEAAGYRVVGGALAGKAAEGLEKEAGIVSRTLSSWELRWKKERDHLDEKTIFVIDEAGMVSSRQLALFVE
AVTKAGAKLVLIGDPDQLQPIEAGAAFRAITDRVGYAELETIYRQKDLWMRDASLDLARGNVARALNAYE
QAGLVQTGWTRKDAIGGLIESWNRDFDPDKTTLILAHRRRDVRELNELARGRLVERGIVGEGFAFNTEDG
PRRFAAGDQIVFLKNEGSLGVKNGMLAKVLDAAPGRIVAEIGDGESRTQVIVEQRFYANVDHGYATTIHK
SQGATVDRVKVLASSTLDRHLTYVAMTRHREAAELHVSMEEFTNRRGGMLVEHGVAPYEHKPGNRDSYYV
TLEDSDGQQRTIWGVDLKRAIEEARPNIGDRIGLKHDGSETVTLPDGTTAERNTWKVIHVAELAIERLKE
RLARDGSKETTLDYESALSYRAALRFAEARGLHLMNVARTISRDRLAWTVRQKQRLIDLAAKLSALGARF
GLTRGSGNISIPNTAKEEKPMVSGITTFPKTIDQAVEDRIAADPALKKQWEEVSMRFHHVYAQPEAAFKA
VDVDAMLSDPATAAKTLSRITAEPETFGALKGKTGLLASRADKLDRDRALKNAAPLADSIGDYLRQRGEA
ERRNHAQELAVRRQVALEIPALSANAKSVLERVRDAIDRNDLPSGLEYALADKMVKAELEGFAKAVTERF
GERTLLPLAAKDTDGEAFQRVTSGMNAAQKSEVQQAWNTMRTVQQLSAHERSVTALKQAETLRQTKSQGL
ALK

  Protein domains


Predicted by InterproScan.

AAA domain (AAA_30)

(382-568)

BID domain of Bartonella effector protein (Bep) (Bep_C_terminal)

(949-1045)

Viral (Superfamily 1) RNA helicase (Viral_helicase1)

(691-732)

MobA/MobL family (MobA_MobL)

(17-243)


  Protein structure



No available structure.




T4CP

ID   5377 GenBank   WP_207591865
Name   traG_BTN45_RS24675_ZX09|unnamed1 insolico UniProt ID   _
Length   644 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 644 a.a.        Molecular weight: 69379.94 Da        Isoelectric Point: 9.0518

>WP_207591865.1 Ti-type conjugative transfer system protein TraG [Rhizobium sp. ZX09]
MNANRIVLVILPAAMIAATVFALTGIETWLAGFGKTEAARLALGRIGVAAPYVASAALGLIFLFATAGSA
SIRAAGLGAVIGNAAVILTVCAREGVRLAAFSSQVPAGKSALSYLDPSTMIGAAAALISGCFAMRVAIIG
NAAFARAAPKRITGKRALHGEADWMTLPEADRLFPATGGIVVGERYRVDRDSVAGVSFRADTTETWGAGG
KSPLLCFDGSFGSSHGIVFAGSGGYKTTSVTIPTALKWGGSLIVLDPSNEVAPMVSRHREEAGRRARILD
PSKPATGFNALDWVGQYGGTKEEDIASVASWIMSDSGRASGVRDDFFRASGLQLLTAIIADVCLSGHTPK
EEQTLRQVRSNLSEPEPKLRQRLQDIYDNSESDFVKENVAAFVNMTPETFSGVYANAIKETHWLSYQNYA
ALVSGSTFRTEDIASGKTDVFINIDLKTLETHSGLARVVIGSFLNAIYNRDGAMDGRALFLLDEVARLGY
MRILETARDAGRKYGITLAMIYQSIGQLRETYGGRDASSKWFESASWISFAAINDPETADYISRRCGVTT
VEIDQVSRSFQSRGSSRTRSKQLAARPLIQPHEVLRMRADEQIVFTAGNPPLRCGRAIWFRRNDMKACVE
QSRFSRREAPAHEQ

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(164-634)

  Protein structure



No available structure.



ID   5378 GenBank   WP_207591876
Name   t4cp2_BTN45_RS24780_ZX09|unnamed1 insolico UniProt ID   _
Length   818 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 818 a.a.        Molecular weight: 91801.56 Da        Isoelectric Point: 5.7692

>WP_207591876.1 conjugal transfer protein TrbE [Rhizobium sp. ZX09]
MAALKRFRATGPSFADLVPYAGLVDNGILLLKDGSLMAGWYFAGPDSESATDLERNELSRQINAILSRLG
TGWMIQVEAIRVPTVDYPTPDQCHFPDPVTRAIDAERRAHFGREQGHFESKHAIILTYRPLESKKTALSK
YIYSDEESRKKSYADTVLFVFRNAIREIEQYLANTLSIARMQTREVRERGGERIARYDDLLQFVRFCITG
ESHPVRLPDVPMYLDWLATAELEHGLTPKVENRFLAVVAIDGLPAESWPGILNNLDLMPLTYRWSSRFIF
LDAEEARQKLERTRKKWQQKVRPFFDQLFQTQSRSVDQDAMTMVAETEDAIAQASSQLVAYGYYTPVVIL
FDEDRDGLQEKAEAIRRLVQAEGFGARVETLNATDAFLGSLPGNWYANIREPLINTSNLADLVPLNSVWS
GNPVAPCPFYPPNSPPLMQVASGSTPFRLNLHVDDVGHTLIFGPTGSGKSTLLALIAAQFRRYEFAQIFA
FDKGNSLLPLTLAAGGDHYEIGNDQNGEGRALAFCPLFDLSTDGDRAWATEWIEMLVALQGVTITPDHRN
AISRQIGLMATAPGKSLSDFVSGVQMREIKDALHHYTVDGPMGQLLDAEEDGLTLRAFQCFEIEQLMNMG
ERNLVPVLTYLFHRIEKRLDGSPSLILLDEAWLMLGHPVFRDKIREWLKVLRKANCAVVLATQSISDAER
SGIIDVLKESCPTKICLPNGAAREPGTREFYERIGFNERQIEIVSGAIPKREYYVATPDGRRLFDMSLGP
VALSFVGASGKENLKRIRALKAEHGQEWPIHWLESRGVHDATSHLNVQ

  Protein domains


Predicted by InterproScan.

CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB)

(192-400)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 41224..55610

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BTN45_RS24695 (BTN45_25310) 39528..40058 + 531 WP_252193175 conjugative transfer signal peptidase TraF -
BTN45_RS24700 (BTN45_25315) 40048..41208 + 1161 WP_207591869 conjugal transfer protein TraB -
BTN45_RS24705 (BTN45_25320) 41224..41832 + 609 WP_207591870 TraH family protein virB1
BTN45_RS24710 (BTN45_25325) 41875..42252 - 378 WP_207591872 DUF5615 family PIN-like protein -
BTN45_RS24715 (BTN45_25330) 42257..42880 - 624 WP_207591873 DUF433 domain-containing protein -
BTN45_RS24720 (BTN45_25335) 43001..43219 - 219 WP_207591874 helix-turn-helix transcriptional regulator -
BTN45_RS24725 (BTN45_25340) 43359..43679 + 321 WP_207591875 transcriptional repressor TraM -
BTN45_RS24730 (BTN45_25345) 43681..44382 - 702 WP_246751768 autoinducer binding domain-containing protein -
BTN45_RS24735 (BTN45_25350) 44604..45485 - 882 Protein_46 IncP-type conjugal transfer protein TrbI -
BTN45_RS24745 (BTN45_25360) 46971..47426 - 456 Protein_48 conjugal transfer protein TrbI -
BTN45_RS24750 (BTN45_25365) 47442..47879 - 438 WP_174019101 conjugal transfer protein TrbH -
BTN45_RS24755 (BTN45_25370) 47883..48695 - 813 WP_174019100 P-type conjugative transfer protein TrbG virB9
BTN45_RS24760 (BTN45_25375) 48712..49374 - 663 WP_062428114 conjugal transfer protein TrbF virB8
BTN45_RS24765 (BTN45_25380) 49395..50573 - 1179 WP_174019099 P-type conjugative transfer protein TrbL virB6
BTN45_RS24770 (BTN45_25385) 50578..50754 - 177 WP_174019098 entry exclusion protein TrbK -
BTN45_RS24775 (BTN45_25390) 50748..51551 - 804 WP_174019097 P-type conjugative transfer protein TrbJ virB5
BTN45_RS24780 (BTN45_25395) 51523..53979 - 2457 WP_207591876 conjugal transfer protein TrbE virb4
BTN45_RS24785 (BTN45_25400) 53988..54272 - 285 WP_246751769 conjugal transfer protein TrbD virB3
BTN45_RS24790 (BTN45_25405) 54269..54649 - 381 WP_174019095 TrbC/VirB2 family protein virB2
BTN45_RS24795 (BTN45_25410) 54639..55610 - 972 WP_174019094 P-type conjugative transfer ATPase TrbB virB11
BTN45_RS24800 (BTN45_25415) 55613..56239 - 627 WP_207591877 acyl-homoserine-lactone synthase TraI -
BTN45_RS24805 (BTN45_25420) 56608..57795 + 1188 WP_207591878 plasmid partitioning protein RepA -
BTN45_RS24810 (BTN45_25425) 57870..58856 + 987 WP_207591880 plasmid partitioning protein RepB -
BTN45_RS24815 (BTN45_25430) 59010..60224 + 1215 WP_207591881 plasmid replication protein RepC -

Host bacterium

ID   8089 GenBank   NZ_CP031365
Plasmid name   ZX09|unnamed1 Incompatibility group   -
Plasmid size   318607 bp Coordinate of oriT [Strand]   35796..35848 [+]
Host baterium   Rhizobium sp. ZX09

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   golT, arsC, arsH
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -