Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 107645 |
| Name | oriT_pPR13E02  |
| Organism | Rhizobium indicum strain JKLM 13E |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP054033 (145630..145658 [+], 29 nt) |
| oriT length | 29 nt |
| IRs (inverted repeats) | 14..19, 24..29 (CGTCGC..GCGACG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 29 nt
>oriT_pPR13E02
AGGGCGCAATATACGTCGCTGCCGCGACG
AGGGCGCAATATACGTCGCTGCCGCGACG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 5138 | GenBank | WP_138331325 |
| Name | traA_FE844_RS30245_pPR13E02 |
UniProt ID | _ |
| Length | 1539 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1539 a.a. Molecular weight: 170200.29 Da Isoelectric Point: 6.5724
>WP_138331325.1 Ti-type conjugative transfer relaxase TraA [Rhizobium indicum]
MAIMFVRAQVISRGAGRSIVSAAAYRHRARMMDEQAGTSFSYRGGASELVHEELTLPDQLPAWLRSAIEG
RSVAGASEVLWNAVDAFEKRADAQLARELIIALPEELTRAENIGLVREFVRDNLTSKGMVADWAYHDKDG
NPHIHLMMTLRPLTEEGFGPKKVAVVGEDGEPLRVVTPDRSKGKIVYKLWAGDKETMKAWKIAWAETANR
HLALAGHEIRLDGRSYAEQGLDGIAQKHLGPEKAALARKGRELYFAPADLARRQEMADRLLAEPELLLKQ
LSNERSTFNECDIAKALHRTVDDPLDFANIRARLMASDDLIMLKPQQVDAETGKAGEPAVFTTRDILRIE
YDMARSAQVLSERRGFAVSAAESLAAAIENVETADPEKSFRLDPEQVDAVRHVTGDNGIAAVVGLAGAGK
STLLAAARVAWESGGRRVIGAALAGKAAEGLEDSSGIRSRTLASWELIWANGQETLHRGDVVVVDEAGMV
SSQQMARVLKLVEQAGAKVVLVGDAMQLQPIQAGAAFRAITERIGFAELAGVRRQREPWAREASRLFARR
EIEKGLDAYAQQGHLLEAETREDIIGRIVEDWTEARRQAIQDLVEAGNVCRLRGDELLVLAHTNEDVKRL
NEALRSVMTGEGALGESRSFQTERGARDFAAGDRIIFLANARFIEPQAMRLGPQYVKNGMLGTVVSTGDK
RGDTLLSVRLDNGRDVVISQDSYRNVDHGYAATIHKSQGSTVERTFVLATGMMDQHLTYVSMTRHRDRAD
LYAAKEDFEVKPEWGRKPRVDHASGVTGELVETGQAKFRPNDEDADDSPYADVKTEDGSVHRLWGVSLPK
ALEEGGVSQGDTVTLRKDGAEKVNVQVPIVDEKTGQKRFEEREVDRNVWTAKQIETAAERQQRIELESHR
PELFRQLVLRLSRSGAKTTTLDFESEASYQAHADDFARRRGIDTLSQAAAGMEEEVSRRWAWIAEKREQV
AELWERASVALGFAIERERRVSYNEERSETQTVSEADAGRGRYLIPPTTVFARSVEEDARQAQLSSPAWK
EREAILRPLLEQIYREPDAALGALNALASDLKIEPRSLTDDLAAAPDRLGRLRGSDLIVDGRVARDERSV
ATAALTDLLPLARAHATEFRRNAERFDLSEQQRRTHMSLPIPALSKTAMVRLMEIEAVRRQGGDDAYKTA
FALAAEDRAVVQEIKAVSEALTARFGWSASTSKADAVAERNVVERMPEDLANTRREKLARLFEAVKRFAD
EQHLAERRDRSKIVAGASADPETESVPMLAAVTEFRTPIDEEARSRALAGPLYRQQRAALADVASTIWRD
PAGAVGKIEDLLANGFAAERIATAVINDPAAYGALRGSDRLMDRMLASGRKRKEAVQAVPDLAARLRALG
SAYVNVLDAERQAITEERRRMAVAIPGLSKAAEDVLMRLTAGAKNNGRRLSALPTPLDPGIRREFAAVSR
ALDERFGRNAILRGEKDLINRIPPAQRRAFEAMQDRLKVLQHAVRLESSEQIISERRQRTVSRGRGIDL
MAIMFVRAQVISRGAGRSIVSAAAYRHRARMMDEQAGTSFSYRGGASELVHEELTLPDQLPAWLRSAIEG
RSVAGASEVLWNAVDAFEKRADAQLARELIIALPEELTRAENIGLVREFVRDNLTSKGMVADWAYHDKDG
NPHIHLMMTLRPLTEEGFGPKKVAVVGEDGEPLRVVTPDRSKGKIVYKLWAGDKETMKAWKIAWAETANR
HLALAGHEIRLDGRSYAEQGLDGIAQKHLGPEKAALARKGRELYFAPADLARRQEMADRLLAEPELLLKQ
LSNERSTFNECDIAKALHRTVDDPLDFANIRARLMASDDLIMLKPQQVDAETGKAGEPAVFTTRDILRIE
YDMARSAQVLSERRGFAVSAAESLAAAIENVETADPEKSFRLDPEQVDAVRHVTGDNGIAAVVGLAGAGK
STLLAAARVAWESGGRRVIGAALAGKAAEGLEDSSGIRSRTLASWELIWANGQETLHRGDVVVVDEAGMV
SSQQMARVLKLVEQAGAKVVLVGDAMQLQPIQAGAAFRAITERIGFAELAGVRRQREPWAREASRLFARR
EIEKGLDAYAQQGHLLEAETREDIIGRIVEDWTEARRQAIQDLVEAGNVCRLRGDELLVLAHTNEDVKRL
NEALRSVMTGEGALGESRSFQTERGARDFAAGDRIIFLANARFIEPQAMRLGPQYVKNGMLGTVVSTGDK
RGDTLLSVRLDNGRDVVISQDSYRNVDHGYAATIHKSQGSTVERTFVLATGMMDQHLTYVSMTRHRDRAD
LYAAKEDFEVKPEWGRKPRVDHASGVTGELVETGQAKFRPNDEDADDSPYADVKTEDGSVHRLWGVSLPK
ALEEGGVSQGDTVTLRKDGAEKVNVQVPIVDEKTGQKRFEEREVDRNVWTAKQIETAAERQQRIELESHR
PELFRQLVLRLSRSGAKTTTLDFESEASYQAHADDFARRRGIDTLSQAAAGMEEEVSRRWAWIAEKREQV
AELWERASVALGFAIERERRVSYNEERSETQTVSEADAGRGRYLIPPTTVFARSVEEDARQAQLSSPAWK
EREAILRPLLEQIYREPDAALGALNALASDLKIEPRSLTDDLAAAPDRLGRLRGSDLIVDGRVARDERSV
ATAALTDLLPLARAHATEFRRNAERFDLSEQQRRTHMSLPIPALSKTAMVRLMEIEAVRRQGGDDAYKTA
FALAAEDRAVVQEIKAVSEALTARFGWSASTSKADAVAERNVVERMPEDLANTRREKLARLFEAVKRFAD
EQHLAERRDRSKIVAGASADPETESVPMLAAVTEFRTPIDEEARSRALAGPLYRQQRAALADVASTIWRD
PAGAVGKIEDLLANGFAAERIATAVINDPAAYGALRGSDRLMDRMLASGRKRKEAVQAVPDLAARLRALG
SAYVNVLDAERQAITEERRRMAVAIPGLSKAAEDVLMRLTAGAKNNGRRLSALPTPLDPGIRREFAAVSR
ALDERFGRNAILRGEKDLINRIPPAQRRAFEAMQDRLKVLQHAVRLESSEQIISERRQRTVSRGRGIDL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 5373 | GenBank | WP_138331294 |
| Name | t4cp2_FE844_RS30030_pPR13E02 |
UniProt ID | _ |
| Length | 561 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 561 a.a. Molecular weight: 61196.55 Da Isoelectric Point: 7.5071
>WP_138331294.1 type IV secretory system conjugative DNA transfer family protein [Rhizobium indicum]
MIHANQIKTAFVNMMRMAARDPLWAIVALITFPLRYARSFLKGAVGYVIVIVTVKFGIDYLLRMILGSSR
GDIVWHIGDWVFMLFAVVLLIWFLSEPLITHFATASDADTHGTARFAGTREIAPLTKAEGGLLIGRANNS
GRLLRYSGPAHLLTMAPTRSGKGVGTIIPNLLTADRSIICIDPKGENAIIAGDVREKFGPVHIVDPFGIT
GRPSAAFNPMDGFNPAGIDVAEDVSTLADALVFDEPGLSGDAHWNEEAKALIAGLLLHVLASQPDDRRTL
TTLRHLLTLAPEAFRAVLDEMQASDAVNGLIARAANRHLGKSDREASGVLSAAQRHTHFLDSPRMTGVLG
RSDFRFGDLKADKATVFLVLPPDRLAAYSRWLRLLVAQSLTEMARTAPSPHPSTPPVLYLLDEFAALGHL
APIERAMGLMAGYGVQLWPIIQDIHQLRATYGQRAGTFLSNAGVLQVFGVNDHDSARLVSDLLGQETVVF
NTAARALDSERTGLSFAEQHVARSLLTPDEVRNMHAKTELLFIAGQRPIVATKLRYYSDPEFVGLFARQE
R
MIHANQIKTAFVNMMRMAARDPLWAIVALITFPLRYARSFLKGAVGYVIVIVTVKFGIDYLLRMILGSSR
GDIVWHIGDWVFMLFAVVLLIWFLSEPLITHFATASDADTHGTARFAGTREIAPLTKAEGGLLIGRANNS
GRLLRYSGPAHLLTMAPTRSGKGVGTIIPNLLTADRSIICIDPKGENAIIAGDVREKFGPVHIVDPFGIT
GRPSAAFNPMDGFNPAGIDVAEDVSTLADALVFDEPGLSGDAHWNEEAKALIAGLLLHVLASQPDDRRTL
TTLRHLLTLAPEAFRAVLDEMQASDAVNGLIARAANRHLGKSDREASGVLSAAQRHTHFLDSPRMTGVLG
RSDFRFGDLKADKATVFLVLPPDRLAAYSRWLRLLVAQSLTEMARTAPSPHPSTPPVLYLLDEFAALGHL
APIERAMGLMAGYGVQLWPIIQDIHQLRATYGQRAGTFLSNAGVLQVFGVNDHDSARLVSDLLGQETVVF
NTAARALDSERTGLSFAEQHVARSLLTPDEVRNMHAKTELLFIAGQRPIVATKLRYYSDPEFVGLFARQE
R
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Host bacterium
| ID | 8080 | GenBank | NZ_CP054033 |
| Plasmid name | pPR13E02 | Incompatibility group | - |
| Plasmid size | 575483 bp | Coordinate of oriT [Strand] | 145630..145658 [+] |
| Host baterium | Rhizobium indicum strain JKLM 13E |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | actP |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |