Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 107640
• Name: oriT_CJ34|unnamed
• Organism: Comamonas fluminis strain CJ34
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP066782 (21074..21212 [-], 139 nt)
• oriT length: 139 nt
• IRs (inverted repeats): 28..34, 38..44 (ACATGGC..GCCATGT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 139 nt

>oriT_CJ34|unnamed
CGAATGGGCAGGCGGGGATTGGCAGGAACATGGCGTAGCCATGTGTATGACAATCCGCATCCTGTTAAGCTCAACCGCTTTACTATCGTATCAGCAGTTTAGGTGCACGGGACGCACTTTGCAACAAGCACAAATGGCG

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_JDW18_RS00140_CJ34|unnamed

• ID: 5134
• GenBank: WP_246609842
• Name: Relaxase_JDW18_RS00140_CJ34|unnamed
• UniProt ID: _
• Length: 1038 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 1038 a.a.
• Molecular weight: 118754.34 Da
• Isoelectric Point: 9.7895

>WP_246609842.1 TraI/MobA(P) family conjugative relaxase [Comamonas fluminis]
MIVKKVPNSKTKHKTGSIRDLADYIREPHNQNPDEKVPYANGRGFLCDGHAAQREEMVALAAESVRSKNP
VNHYIMSWREGEQPSPEQVEEAVSIFMEELGWKDHQAIYGLHKDTDNIHLHIAINRVHPEKLKIVEINRG
FDIEMAHKAIARIEHAQGWQREQNGRYQVLENGELGRAPYDPEKPRQPDQKKRDMENRTGEKSAHRIAIE
DGAAIIKQAQSWEQLHRELAAKGMRYEKTGSGATVFVGDVGVKASDVDRNASLAKMQKRLGEYQPAPQHQ
QVAQREPEPIKPDVPGWKDYITGRKAHYAAKNAAKLALDKQQEQERKQLAEQQKARRDELMRGNWKGKGE
VLNAMRSVIAAEQAAEKAALKEKHQKQREQWRQQFRPYPDLEQWQRMQKSPELAEQWRHRASEPQRIEGD
RSEPPTVRDIRAYVPEIVGQQVHYSRKDEAGRGGGVSFVDKGKSIDIHDWRNRDSTLAALQLSAQKWGSF
TVTGCDEYKTMCVKLAAEHGFKITNPELQESIQRERQRIQQERAQAMKSEQLKQFERYAEAVGAERYRVT
SIKMREDGGKQTFILDKKDGITRGFTPQEIEQRTPEMQRLQRRGENLYYTPLSDKKHHILIDDMSREKLE
RLIKDGYQPAAVLESSPGNYQAIITVPKLGTVHDKDVGNRLSDALNREYGDPKLSGAIHPHRAPGYENRK
PKHQREDGSYPEVRLLKAERRECVKALALSSQIDAEYQRQAALKAQQPERSKAKPALELAAASGSAIDAY
QRHYRDVLKRQRGGEVDLSRVDSMIAVRMRVTGHDQAAIEGAIRQCAPATRQKDEGRDWNDYAQRTARYA
YSAAGDRQAAELGKYRQQWEKLEGEGRGARLERLRAAHRPLCLQRRRRPASRRAWEVPPAVGEAGRARAC
TPAGAGQGAEDRARQLTRHESIRLDVTTKRRYNKNAMELEWDADKAARNLKKHGVSFEDAELVFYDSGRV
EAYDGREDYGEDRWATIGLAYSAVLYVVYTVRHEETIRLISARKANADERKQYREANS

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (17-273)

• Large polyvalent protein-associated domain 7 (LPD7) (442-526)

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (BrnT_toxin) (951-1029)

• RepB DNA-primase from phage plasmid (RepB_primase) (547-806)

Protein structure

No available structure.

Host bacterium

• ID: 8075
• GenBank: NZ_CP066782
• Plasmid name: CJ34|unnamed
• Incompatibility group: -
• Plasmid size: 52138 bp
• Coordinate of oriT [Strand]: 21074..21212 [-]
• Host baterium: Comamonas fluminis strain CJ34

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -