Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 107638
• Name: oriT_pRL4b6
• Organism: Rhizobium leguminosarum bv. trifolii strain 4B
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP050105 (355440..355468 [+], 29 nt)
• oriT length: 29 nt
• IRs (inverted repeats): 14..19, 24..29 (CGTCGC..GCGACG)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 29 nt

>oriT_pRL4b6
AGGGCGCAATATACGTCGCTGTCGCGACG

oriT secondary structure

Predicted by RNAfold.

Relaxase

traA_HA460_RS31090_pRL4b6

• ID: 5132
• GenBank: WP_166465927
• Name: traA_HA460_RS31090_pRL4b6
• UniProt ID: _
• Length: 1538 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 1538 a.a.
• Molecular weight: 169692.64 Da
• Isoelectric Point: 7.2399

>WP_166465927.1 Ti-type conjugative transfer relaxase TraA [Rhizobium leguminosarum]
MAIMFVRAQVIGRGAGRSVVSAAAYRHRARMMDEQAGTSFSYRGGASELVHEELALPDQIPAWLRSAISG
QSVAKASEALWNAVDAFEKRADAQLARELIIALPEELTRAENIALVREFVLDNLTAKGMVADWVYHDKDG
NPHIHLMTTLRPLAEEGFGPKKVPVCGEDGKPLRVVTPDRPNGKVVYKLWAGDKETLQAWKIAWAETASR
HLALAGHEIRLDGRSYAEQGLDGIAQKHLGPEKAALSRRDAEIYFAPADLARRQEMADRLLADPALLLKQ
LSNERSTFDERDIAKALHRTVDDPADFANIRARLMASDDLIMLKPQQIDAETGKASEPAVFTTREMLRIE
YDMGRSARVLAERRGFAVSDRVVAAAIRQVETQDPENSFRLDPEQVDAIHHITRDDGIAAVVGLAGAGKS
TLLAAARVAWESDGRRVIGAALAGKAAEGLEDSSGIRSRTLAAWELAWASGRELLERGNVLVIDEAGMVS
SQQMARLLDIAEQAEVKVVLVGDAMQLQPIQAGAAFRAITERIGFAELAGVRRQRQPWAREASRLFARGE
TGKGLDAYAQQGHLIEAGTRVETIDRIVADWSQARKQAIQNSTTAGNDGRLRGDELLVLAHTNEDVKYLN
EALRSVMVGEGALGEDRAFRTERGARAFAAGDRIIFLENARFVEPRATRLGPQHVKNGMLGTVVSTGDKR
GDAVLSIRLDSGRDVVISESSYRNVDHGYAATIHKSQGATVERTFVLATGMMDQHLTYVSMTRHRDRVDL
YAAKDDFEAKPEWGRKPHVDHAAGVTGALVETGQAKFRPNDEDADDSPYADVRTDDGTLHRLWGVSLPKA
LEEGGVSEGDTVTLRKDGVERVTIKVAIVDEQTGQKRFEEREVDRNVWTAKRIETAEARQQRIERESHRP
DVFKQLVERLSRSGAKTTTLDFESEAGYRAHADDFARRRGLDHLSLAAAGAEESLSRRWAGIAEKREQLA
KLWERASVALGFTIERERRIAYNEERAEPQTVLEADVARGRYLIPPTTGFARSAEEDAQLAQLSSPAWKE
REAILRPLLEKIYRDPDAALVALNALSSDTSVDPRRLADDLAKAPERLGRLRGSELIVDGRAARDERNAA
ITALTDLLPLVRAHATRFRRNAERFESHEQQRRAHMALSIPALSEKAMARLTEIEAVRRQGGDDAYKAAF
ALAAEDRSIVQEVKAVSEALTARFGWSAFTSKADAVGERNMVERMPEDLASSRGEKLTRLFKAVKRFADE
QHLAERRDRSKIVAGASADQGMENVPMLAAVTEFRTAIDEEARSRVLSDPLYRQQRAALAAVASAIWRDP
VGAVGKIEELLQKGFAGERIAAAVTNDPAAYGALRGSDRLMDRMLAAGRERKQAVQAVPEAGARLRSLGS
AYVNVLDAERQAIAEERRRMAVAIPALSTAAEDVLLRLTAEAKNNDRKLTASAAPLDPSIRREFAAVSRA
LDERFGRNAIIRGEKDLINRVPQAQRSAFEAMQDRLKVLQRTVRMESSEQIISERRQRAVSRGRGIEL

Protein domains

Predicted by InterproScan.

• MobA/MobL family (MobA_MobL) (17-249)

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (1305-1399)

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (1047-1127)

• AAA domain (AAA_30) (391-576)

Protein structure

No available structure.

Host bacterium

• ID: 8073
• GenBank: NZ_CP050105
• Plasmid name: pRL4b6
• Incompatibility group: -
• Plasmid size: 693075 bp
• Coordinate of oriT [Strand]: 355440..355468 [+]
• Host baterium: Rhizobium leguminosarum bv. trifolii strain 4B

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: actP
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA9