Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 107637 |
| Name | oriT_Corn53|AB  |
| Organism | Ensifer adhaerens strain Corn53 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP030264 (932985..933023 [+], 39 nt) |
| oriT length | 39 nt |
| IRs (inverted repeats) | 23..28, 33..38 (CGTCGC..GCGACG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 39 nt
>oriT_Corn53|AB
GGATCCGAAGGGCGCAATTATACGTCGCTGACGCGACGC
GGATCCGAAGGGCGCAATTATACGTCGCTGACGCGACGC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 5131 | GenBank | WP_112772796 |
| Name | traA_DQW09_RS32085_Corn53|AB |
UniProt ID | _ |
| Length | 1103 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1103 a.a. Molecular weight: 121779.02 Da Isoelectric Point: 9.9000
>WP_112772796.1 Ti-type conjugative transfer relaxase TraA [Ensifer adhaerens]
MAAPHFSVSIVARGSGRSAVLSAAYRHCAKMDYKREARTIDYTRKQGLLHEEFVIPADAPEWLQSMIAYH
SVSGTSEAFWNKVEDFEKRSDAQLAKDVTIALPIELSAAQNIELVRDFVSRHITGQGMVADWVFHDAPGN
PHIHLMTTLRPLTEDGFGSKKVAVTGPDGKPLRNDAGKIVYQLWAGSLNDFIVFRDGWFACQNRHLALAG
LDLRVDGRSFEKQGIALMPAIHLGVGAKAMERKATGCGEVAALERLELQAERRAENARRIQRNPGIVLDL
LTREKSVFDSQDIAKILHRYIDDAGHFQNLMARILESPETLRLNLERVDFATGLRAPAKYTTRELIRLEA
GMANRAIWLSQRSSHEVRRPVLDATFARHDRLSDEQKTAIEHVVGKARIALVIGRAGAGKTTMMKAAREA
WEAAGYRVVGGALAGKATEGLEKEAGIASRTLASWEMCWGKGREQLDDRCVFVLDEAGMVSSRQMALFVE
AVTSCGAKLVLVGDPEQLQPIEAGAAFRAIADRIGYAELEIVYRQREQWMRDASLDLARSNVGKAVEAYR
ANGRMIGAELKSEAVAALIADWNRDFDPAKSSLILAHLRRDVRMLNELARAKLIQRGIIESGATFKTADG
DRSFAAGDQIVFLKNEGSLGVKNGMLAKVADAAPGRIVAEIGDGKHRRQISVDQRFYSNVDHGYATTIHK
SQGATVDRVKVLASLSLDRHLTYVAMTRHREDLGVYYGRRSFAKAGGLIAILSRKNSKETTLDYVNGSFY
RQALLFAEARGFHLVKVARTLARDRLVWVVRQKQRLVELGAQLSALGKALGLSPIKKSSETASAINEAKP
MVSGIKVFPKSLEQTVEDRLAADPGLKKQWEEVSSRFHFVYAEPEAAFKAINVDAILSDEAAAKSAIAKI
NTEPESFGALKGKTGMFVSSAHKQDREKAILNAPALARNLERYLRHRAVAERKHETEERSIRAKFSIDIP
ALSPSAKQTLERVRDAIDRNDLPAGLEFALAEGQVKAELEGFTRAVSERFGERTFLTLAAKEPNGETFQK
LTSDMNPSQKGEVQSAWNAMRTAQQLAAHEHTMTALKQSETSRQTKSQGLSLK
MAAPHFSVSIVARGSGRSAVLSAAYRHCAKMDYKREARTIDYTRKQGLLHEEFVIPADAPEWLQSMIAYH
SVSGTSEAFWNKVEDFEKRSDAQLAKDVTIALPIELSAAQNIELVRDFVSRHITGQGMVADWVFHDAPGN
PHIHLMTTLRPLTEDGFGSKKVAVTGPDGKPLRNDAGKIVYQLWAGSLNDFIVFRDGWFACQNRHLALAG
LDLRVDGRSFEKQGIALMPAIHLGVGAKAMERKATGCGEVAALERLELQAERRAENARRIQRNPGIVLDL
LTREKSVFDSQDIAKILHRYIDDAGHFQNLMARILESPETLRLNLERVDFATGLRAPAKYTTRELIRLEA
GMANRAIWLSQRSSHEVRRPVLDATFARHDRLSDEQKTAIEHVVGKARIALVIGRAGAGKTTMMKAAREA
WEAAGYRVVGGALAGKATEGLEKEAGIASRTLASWEMCWGKGREQLDDRCVFVLDEAGMVSSRQMALFVE
AVTSCGAKLVLVGDPEQLQPIEAGAAFRAIADRIGYAELEIVYRQREQWMRDASLDLARSNVGKAVEAYR
ANGRMIGAELKSEAVAALIADWNRDFDPAKSSLILAHLRRDVRMLNELARAKLIQRGIIESGATFKTADG
DRSFAAGDQIVFLKNEGSLGVKNGMLAKVADAAPGRIVAEIGDGKHRRQISVDQRFYSNVDHGYATTIHK
SQGATVDRVKVLASLSLDRHLTYVAMTRHREDLGVYYGRRSFAKAGGLIAILSRKNSKETTLDYVNGSFY
RQALLFAEARGFHLVKVARTLARDRLVWVVRQKQRLVELGAQLSALGKALGLSPIKKSSETASAINEAKP
MVSGIKVFPKSLEQTVEDRLAADPGLKKQWEEVSSRFHFVYAEPEAAFKAINVDAILSDEAAAKSAIAKI
NTEPESFGALKGKTGMFVSSAHKQDREKAILNAPALARNLERYLRHRAVAERKHETEERSIRAKFSIDIP
ALSPSAKQTLERVRDAIDRNDLPAGLEFALAEGQVKAELEGFTRAVSERFGERTFLTLAAKEPNGETFQK
LTSDMNPSQKGEVQSAWNAMRTAQQLAAHEHTMTALKQSETSRQTKSQGLSLK
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 5367 | GenBank | WP_112772798 |
| Name | traG_DQW09_RS32070_Corn53|AB |
UniProt ID | _ |
| Length | 638 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 638 a.a. Molecular weight: 68649.42 Da Isoelectric Point: 9.3513
>WP_112772798.1 Ti-type conjugative transfer system protein TraG [Ensifer adhaerens]
MTATRTLLVVAPVALMVTAIFVTTGMETWLQGFGNTEAAKVALGRVGISAPYIAASAVGVVFLFASAGSA
SVRFSGWGVVAGSGATILIAALREAVRLAAFAGKMPAGKNLAASLDPATVLGAAIALMAASFALRVVLIG
NAAFARPEPKRVNGRRALHGEADWMKLASAARLFPESGGIVVGELYRVDKDSTAAQPFRADSPDTWGAGG
KSPLLCFDGSFGSSHGIVFAGSGGFKTTSVTIPTALKWGGSVVILDPSKEVAPMVSKHRRDADRDVFVLD
PMAPETGFNALDWVGRFGGTKEEDIASVASWIMSESGGVRGVRDDFFRASALQLLTALIADVCLSGRTDK
ADQTLRQVRKNLSEPEPKLRERLQTIYDNSDSDFVKENVAAFVNMTPETFSGVYANAVRETHWLSYSNYA
ALVSGSTFSTGEIATGHADVFINVDLKTLEGHSGLARVIIGALLNAIYNRNGEMNGRTLFLLDEVSRLGY
MRVLETARDAGRKYGITLTMIYQSIGQMREAYGGRDAASKWFESVSWISFAAINDPETADYISRRCGMTT
VEIDQVSRSFQSRGSSRTRSKQLAARPLIQPHEVLQMRADEQIVFTAGNPPLRCGRAIWFRRADMKSCVA
ENRFEKMK
MTATRTLLVVAPVALMVTAIFVTTGMETWLQGFGNTEAAKVALGRVGISAPYIAASAVGVVFLFASAGSA
SVRFSGWGVVAGSGATILIAALREAVRLAAFAGKMPAGKNLAASLDPATVLGAAIALMAASFALRVVLIG
NAAFARPEPKRVNGRRALHGEADWMKLASAARLFPESGGIVVGELYRVDKDSTAAQPFRADSPDTWGAGG
KSPLLCFDGSFGSSHGIVFAGSGGFKTTSVTIPTALKWGGSVVILDPSKEVAPMVSKHRRDADRDVFVLD
PMAPETGFNALDWVGRFGGTKEEDIASVASWIMSESGGVRGVRDDFFRASALQLLTALIADVCLSGRTDK
ADQTLRQVRKNLSEPEPKLRERLQTIYDNSDSDFVKENVAAFVNMTPETFSGVYANAVRETHWLSYSNYA
ALVSGSTFSTGEIATGHADVFINVDLKTLEGHSGLARVIIGALLNAIYNRNGEMNGRTLFLLDEVSRLGY
MRVLETARDAGRKYGITLTMIYQSIGQMREAYGGRDAASKWFESVSWISFAAINDPETADYISRRCGMTT
VEIDQVSRSFQSRGSSRTRSKQLAARPLIQPHEVLQMRADEQIVFTAGNPPLRCGRAIWFRRADMKSCVA
ENRFEKMK
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 938145..949821
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| DQW09_RS32090 (DQW09_32280) | 936475..936975 | + | 501 | WP_234824570 | conjugative transfer signal peptidase TraF | - |
| DQW09_RS32095 (DQW09_32285) | 936965..938131 | + | 1167 | WP_159416286 | conjugal transfer protein TraB | - |
| DQW09_RS32100 (DQW09_32290) | 938145..938747 | + | 603 | WP_029742461 | TraH family protein | virB1 |
| DQW09_RS32105 (DQW09_32295) | 938771..939859 | - | 1089 | WP_112772793 | GSU2403 family nucleotidyltransferase fold protein | - |
| DQW09_RS32110 (DQW09_32300) | 940344..941639 | - | 1296 | WP_029742464 | IncP-type conjugal transfer protein TrbI | virB10 |
| DQW09_RS32115 (DQW09_32305) | 941652..942089 | - | 438 | WP_029742465 | conjugal transfer protein TrbH | - |
| DQW09_RS32120 (DQW09_32310) | 942093..942905 | - | 813 | WP_159416439 | P-type conjugative transfer protein TrbG | virB9 |
| DQW09_RS32125 (DQW09_32315) | 942922..943584 | - | 663 | WP_060644937 | conjugal transfer protein TrbF | virB8 |
| DQW09_RS32130 (DQW09_32320) | 943604..944788 | - | 1185 | WP_159416440 | P-type conjugative transfer protein TrbL | virB6 |
| DQW09_RS32135 (DQW09_32325) | 944793..944969 | - | 177 | WP_159416287 | entry exclusion protein TrbK | - |
| DQW09_RS32140 (DQW09_32330) | 944963..945709 | - | 747 | WP_159416288 | P-type conjugative transfer protein TrbJ | virB5 |
| DQW09_RS32145 (DQW09_32335) | 945737..948186 | - | 2450 | Protein_904 | conjugal transfer protein TrbE | - |
| DQW09_RS32150 (DQW09_32340) | 948197..948511 | - | 315 | WP_060644942 | conjugal transfer protein TrbD | virB3 |
| DQW09_RS32155 (DQW09_32345) | 948483..948863 | - | 381 | WP_113189234 | TrbC/VirB2 family protein | virB2 |
| DQW09_RS32160 (DQW09_32350) | 948853..949821 | - | 969 | WP_159416441 | P-type conjugative transfer ATPase TrbB | virB11 |
| DQW09_RS32165 (DQW09_32355) | 950230..951156 | + | 927 | WP_244495328 | nucleotidyltransferase and HEPN domain-containing protein | - |
| DQW09_RS32170 (DQW09_32360) | 951588..951923 | + | 336 | WP_134887825 | helix-turn-helix transcriptional regulator | - |
| DQW09_RS32175 (DQW09_32365) | 951916..953163 | + | 1248 | WP_159416289 | HipA domain-containing protein | - |
| DQW09_RS32180 (DQW09_32370) | 953502..954200 | - | 699 | WP_089045403 | LuxR family transcriptional regulator | - |
Host bacterium
| ID | 8072 | GenBank | NZ_CP030264 |
| Plasmid name | Corn53|AB | Incompatibility group | - |
| Plasmid size | 1583018 bp | Coordinate of oriT [Strand] | 932985..933023 [+] |
| Host baterium | Ensifer adhaerens strain Corn53 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | arsC |
| Degradation gene | - |
| Symbiosis gene | fixB, fixA |
| Anti-CRISPR | AcrIIA7 |