Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 107617
• Name: oriT_pCRULAC1
• Organism: Corynebacterium crudilactis strain JZ16
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP015623 (131630..131733 [+], 104 nt)
• oriT length: 104 nt
• IRs (inverted repeats): 63..70, 73..80 (CACCATGC..GCATGGTG)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 104 nt

>oriT_pCRULAC1
GCCCCGCAGGGCAGGATTCCCGTTGAGCGCCGCAGGTGCGAATAAGGGGAAGTGAAGAGGAACACCATGCTTGCATGGTGGGCCTACTTCACACATCCTGCCCG

oriT secondary structure

Predicted by RNAfold.

T4CP

tcpA_ccrud_RS14055_pCRULAC1

• ID: 5348
• GenBank: WP_066570134
• Name: tcpA_ccrud_RS14055_pCRULAC1
• UniProt ID: _
• Length: 842 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 842 a.a.
• Molecular weight: 94386.41 Da
• Isoelectric Point: 8.7106

>WP_066570134.1 FtsK/SpoIIIE domain-containing protein [Corynebacterium crudilactis]
MATKTRRKNPNSMSSQLADSAKKRSGQLFLDWATLTWNKDTTKIGPLPIIAYNWALLVIFPALGYFLPTH
RLAVATACALWILTTIGRAAAVQPARRKTLLRIYEQNQRSAALPRITPTNGLSPKKQIQVRRWGKKSQPT
DLTLKIGDCPAASSMYARTQVEKALISSLDAPPQGQTWLITWPSPTVVRCHTVDEHGEEASRQKFTRKVH
GRTAQVFKINMRDSADYAYACSDWFSTESKTGEMVQTPKKIILTLGSFNYADMFARKMVERGLDDLLKVP
GSWIYSWGEGDLEMTQVERKSVEARRKNTTRKINDDIGGLVQGRGDMPIVDVKKWSDFEDHSDYPEEIII
NFGTRNLSERRLRDNMENSLDTSMEAMYQGSTWLYDWIPSGASTVLHAKAVAKDSLQSSRKYATKRLRNV
VEAKFGSSRNFVDCDIVEWQEELAKTGESLPKEATVNFGDVDVTKQETQDSFEMHWDSLTTACDWHYRWN
PAEGFVTMTAVPPLPPYAFFPKPGTPEFDKWNADARDGKINFGLRKGGGHLIWDFNKTPHGLIGGKTGMG
KSVSLNIIAFAAMYNPDLLELVVCDPKRTDFTWTPEFPNVIKFAALDPEIVAAVAMAKNEMERRQSLLAR
VGVRNIGELRRKLAENPELVAEFGAPPRRLVLFFDELADFLAKGADKDNEELKDEARSDLEKIARLGRAM
EVNIVAAAQKPDSKIVSTQLRSQLGFRLAVGPTDRHESEMILNSDHGTRHPASGVPKGRAWGYDPLGGYQ
LCQVFFLADNSGHLDWKPDEYMLGFKDMVRDRLKHLGYASHTITNSDGGSEGRWAKVEGVGVDPNAPTIA
MT

Protein domains

Predicted by InterproScan.

• FtsK/SpoIIIE family (FtsK_SpoIIIE) (520-727)

Protein structure

No available structure.

t4cp2_ccrud_RS14430_pCRULAC1

• ID: 5349
• GenBank: WP_066570416
• Name: t4cp2_ccrud_RS14430_pCRULAC1
• UniProt ID: _
• Length: 828 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 828 a.a.
• Molecular weight: 89503.18 Da
• Isoelectric Point: 5.0037

>WP_066570416.1 hypothetical protein [Corynebacterium crudilactis]
MGLFGSSKADDTDSKKSEKKSGGIVLPLTVAGVAAMALEPLVLPIFVTLVIVGERRKIRLSRWWLATGVG
TIIALAASGLNFSRLGSWFLSAPAALWGSRIPDSLDVPVLPTIAAMSSDTSTALLWLQHCLVAAPTALFG
MSLWWWWRSYALQILGENEGAKYSNRRPVGWLDRRRIKRNEIAVRSGDWSQKNPGGIALGIGTYGDVAST
VVDDVKKAVAVVGTSRSGKTRLANSIAAQQVHEMQGGHITICYKGADDVARAKAELAHEMGVPFLHFKLM
PPTGGGYKAPHPYAPNRPAHYDPFQHGNGASKAAMLLNSVPRDGDAAVYERKASEAVKLAYDIAALTGFD
KQKTETGAPVSGLAVLARLLDPDTLVKMGLSVTVEQVRGAHPYLSQAAAESKVGAIHSRIEAFKIELGRS
SSTLSGALEDVRSTVASYVNDSAAGSFLSPGSIPSLRIDLVRAILRNEIIVFSLPAQEYPEMASMIGTMV
LLDLQNAVATLRDKRNIIAERYDNEGEQRPGGPDGTPWNPLVVQLEEIGSVKSTAAAEAMLGLFNKSADV
GIRPILSTQSLSDIEAVDGSGVWLRQLTAQLDHLITFQLSESHDSEKVAAFSGMVEKKLPTESNSVENNR
TGLFRSATAVNDLRSQPQELNRIPVGEALGLDRSLGQLLWVSKVPKLSAVHTTGKEGPNNWFEALQIAPV
YEKPQDWNPFDDADMVAQYREDRDDSFKALLKDLEEDAVLHQVLDDDEESVDIEQGVITPKPSSLTPIPA
PQEPSTPHDPMPVDSAAEFHPTDEDIPAHLDSHDDPYSDPYGGSTIETFDDGKDNDLW

Protein domains

No domain identified.

Protein structure

No available structure.

Host bacterium

• ID: 8052
• GenBank: NZ_CP015623
• Plasmid name: pCRULAC1
• Incompatibility group: -
• Plasmid size: 144640 bp
• Coordinate of oriT [Strand]: 131630..131733 [+]
• Host baterium: Corynebacterium crudilactis strain JZ16

Cargo genes

• Drug resistance gene: aph(4)-Ia, aac(3)-IVa, aph(3')-Ia, aph(3'')-Ib, aph(6)-Id, sul2
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -