Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 107602
• Name: oriT_pRLN2
• Organism: Rhizobium leguminosarum strain Norway
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP025014 (240193..240221 [+], 29 nt)
• oriT length: 29 nt
• IRs (inverted repeats): 14..19, 24..29 (CGTCGC..GCGACG)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 29 nt

>oriT_pRLN2
AGGGCGCAATATACGTCGCTGTCGCGACG

oriT secondary structure

Predicted by RNAfold.

Relaxase

traA_CUJ84_RS30675_pRLN2

• ID: 5111
• GenBank: WP_105009385
• Name: traA_CUJ84_RS30675_pRLN2
• UniProt ID: _
• Length: 1545 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 1545 a.a.
• Molecular weight: 170663.80 Da
• Isoelectric Point: 7.1240

>WP_105009385.1 Ti-type conjugative transfer relaxase TraA [Rhizobium leguminosarum]
MAIMFVRAQVISRGAGRSIVSAAAYRHRTRMMDEQAGTSFSYRGGASELVHEELALPDQMPTWLRTAIDG
RSVAGASEALWNAVDAFEKRADAQLARELIIALPEELTRAENIALVREFVRDNLTSNGMVADWVYHDRDG
NPHIHLMTTLRPLTEEGFGQKKVAVTGDDGAPLRVVTPDRPKGRIVYRLWAGDKETMKAWKIAWAETANR
HLALAGHEIRLDGRSYAEQGLDGIAQTHLGPEKAALVRKGRELHFAPADLARRQHMADRLLADPALLLKQ
LSNERSTFDERDIAKALHRIVDDPAVFANIRARLMASDELVMLKPQQIDAETGKASEPAVFTTRAILRIE
YDMARSAQLLAERRGFAVSDRVVAAAIRNVEARDPEKPFKLDPEQVDAVRHVTGDNGIAAVVGLAGAGKS
TLLAAARVAWESDGRRVIGAALAGKAAEGLEDSSGIRSRTLASWELAWANGRELLERGNVLVIDEAGMVS
SQQLARVLKIAEQAQAKVVLVGDAMQLQPIQAGAAFRAITERIGFAELAGVRRQRQQWAREASRLFARGE
TEKGLDTYARHGHLIEAGTRDDIIGRIVGDWTEARRQALQNSAAEGNDGRLRGDELLVLAHTNQDVKRLN
EALRSVMTGEGALGESGSFQTERGAREFAVGDRIIFLENARFLEPRAPRLGPQHVKNGMLGTVVSTGDKR
ADTLLSVRLDNGRDVVISEDSYRNVDHGYAATIHKSQGSTVERTLVLATGMMDQHLTYVSMTRHRDRADL
YAAKEDFEAKPEWGRKPHVEHAAGVTGALVESGQAKFRPNDEDADDSPYADVKTDDGTIHRLWGVSLPKA
LEDAGVSEGDTVTLRKDGVERVTIKVATVDEQTGQKRFEEREVERNVWTAKQIETAEERHERIEQESHRP
DLFRQLVERLSRSGAKTTTLDFESEAGYRAHADDFARRRGLDHLSLAAAGVEGSLSRRWAGIAERREQLA
KLWERAGVAFGFTIERERQVAYSEERAETQTVLDADVVRGRYLIPPVTVFARSVEEDARLAQLASPAWKE
REAILRPLLETIYRDPDAALVALNALSSDTSIEPRRLADDLAKAPERLGRLRGSDLIVDGRAARDERNAA
IAVLTDLLPLARAHATEFRRNGERFDLHEQQRRTHMSLPIPALSKTAMARLTEIEAVLKQGGDDAYKTAF
AIAVEDRAVVQEIKAISEALTARFGWSAFTPKTDTVAERNVIERMPEDLASIRGEKLTRLFEAVKRFADE
QHLVERRDRSKIVAAASADQGMEADKESAAVLPMLAAVTEFKTPVDEEARSRVLSDPLYRQQRAALATVA
STIWRDPAGAVGKIEELLQKGFAAERIAAAATNDPSAYGALRGSDRLMDRLLASGRERRAAVQAVPEAGA
RLRALGSAYVNVLDAERQVVAEERRRMAVAIPGLSKAAEDVLMRLSAEARINGRKLSAYAASLDPSIRRE
FAAVSTALDERFGRNAIIRGEKDLINRVPQAQHRAFEAMQERLKVLQQAVRRESSEQIISERRQWAVNRG
RGVGL

Protein domains

Predicted by InterproScan.

• MobA/MobL family (MobA_MobL) (17-249)

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (1045-1126)

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (1312-1405)

• AAA domain (AAA_30) (391-576)

Protein structure

No available structure.

Host bacterium

• ID: 8037
• GenBank: NZ_CP025014
• Plasmid name: pRLN2
• Incompatibility group: -
• Plasmid size: 592529 bp
• Coordinate of oriT [Strand]: 240193..240221 [+]
• Host baterium: Rhizobium leguminosarum strain Norway

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: actP
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -