Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 107598 |
| Name | oriT_pSK04  |
| Organism | Rhizobium leguminosarum bv. viciae strain BIHB 1148 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP022568 (235127..235155 [-], 29 nt) |
| oriT length | 29 nt |
| IRs (inverted repeats) | 14..19, 24..29 (CGTCGC..GCGACG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 29 nt
>oriT_pSK04
AGGGCGCAATATACGTCGCTGTCGCGACG
AGGGCGCAATATACGTCGCTGTCGCGACG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 5108 | GenBank | WP_094088885 |
| Name | traA_CHY08_RS32495_pSK04 |
UniProt ID | _ |
| Length | 1545 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1545 a.a. Molecular weight: 171056.37 Da Isoelectric Point: 6.7275
>WP_094088885.1 Ti-type conjugative transfer relaxase TraA [Rhizobium leguminosarum]
MAIMFVRAQVISRGVGRSIVSAAAYRHRARMMDEQAGTSFSYRGGAPELVHEEVALPDWTPAWLRAAIDG
RTVAAASEALWNAVDVFEKRADAQLARELIIALPEELTRAENIALVREFVRDNLTSKGMVADWVYHDKDG
NPHIHLMTTLRPLTQEGFGRKKVAVTGEDGAPLRVVTPDRPKGRIVYKLWAGDKETLQAWKVAWAETANR
HLALAGHEIRLDGRSYAEQGLDGIAQKHLGPEKAALSRRGAEMYFAPADLARRQEMADRLLADPTLLLKQ
LGNERSTFDERDIAKALHRYVDDPADFANIRARLMASDDLVMLKPQQVDAETGKASEPAVFTTREILRVE
YDMSQSARVLSERRGFAVSAESVAAAIENVETADPEKSFRLDAEQVDAVRHITGDSGIAAVVGLAGAGKS
TLLAAARLAWEHDGRRVIGAALAGKAAEGLEDSSGIRSRTLASWELIWADGHETLHRGDVIVIDEAGMVS
SQQMARVLKLVEQAGAKVVLVGDAMQLQPIQAGAAFRAITERIGFAELAGVRRQREDWAREASRLFARGE
IEKGLDAYAQQGHLIEAATRDEIIDRIVADWSEARKLAIETSVLEGNDGRLRGDELLVLAHTNDDVKRLN
EALRSVMTGEGALGEGRSFRAERGAREFAAGDRIIFLENARFLEPRAPRLGPQYVKNGMLGTVVSTGDKR
GDALLSVRLDNGRDVVISEDSYRNVDHGYAATIHKSQGATVERTFVLATGMMDQHLTYVSMTRHRDRADL
YAAKEDFEARPEWGRKPRVDHAAGVTGELVETGQAKFRPNDEDADDSPYADVKTDDGAVHRLWGVSLPKA
LEESGVSQGDTVTLRKDGVERVKVQVPIVDEQTGQKRFEEREVDRNVWTAKRIETAEARLERIERESHRP
DVFKQLVERLSRSGAKTTTLDFESEAGYRAHADDFARRRGLDTLSRAAAGMEEEVSRRWAWIAEKREQVA
ELWERASMALGFAIERERRVSYNEERIETQSVSDADAGRARYLIAPTTVFARSVDEDARLAQLASPTWKE
REAILRPLLEKIYRDPDAALVALNALSSDAGLEPRKLAEDLAAAPDRLGRLRGSDLIVDGRAARDERSVA
TAALLELIPLARAHATEFRRNAERFDLREQQRRAHMSLPIPAPSRKAMKRLVEIEAVRRQGGDDAYKSAF
ALATEDRSLVQEVKAVSEALTARFGWSAFTSKADAVAERNVVERMPEDLASIRGEKLTRLFEAVKRFAEE
QHLVERRDRSKIVAGASADQGMEAGKENAAVLPMLAAVTEFKTPIDEEARLRTLATPLYRQQRAALATVA
SMIWRDPAGAVSKIEELLQKGFAGERIAAAVTNDPAAYGALRGSDRLMDRMLASGRERKEAVQAMPEAAA
RLRALGSAYVNVLDAERQAITEERRRMTVAIPGLSKAAEEALMRLTAEAKNNGRKLSAPAASLDPGIRRE
FDAVSRALDERFGRNAIVRGEKDLINRIPPLQRRAFEALQDRLKVLQQAVRMESSEQIISERRQRAVSRG
RGIDL
MAIMFVRAQVISRGVGRSIVSAAAYRHRARMMDEQAGTSFSYRGGAPELVHEEVALPDWTPAWLRAAIDG
RTVAAASEALWNAVDVFEKRADAQLARELIIALPEELTRAENIALVREFVRDNLTSKGMVADWVYHDKDG
NPHIHLMTTLRPLTQEGFGRKKVAVTGEDGAPLRVVTPDRPKGRIVYKLWAGDKETLQAWKVAWAETANR
HLALAGHEIRLDGRSYAEQGLDGIAQKHLGPEKAALSRRGAEMYFAPADLARRQEMADRLLADPTLLLKQ
LGNERSTFDERDIAKALHRYVDDPADFANIRARLMASDDLVMLKPQQVDAETGKASEPAVFTTREILRVE
YDMSQSARVLSERRGFAVSAESVAAAIENVETADPEKSFRLDAEQVDAVRHITGDSGIAAVVGLAGAGKS
TLLAAARLAWEHDGRRVIGAALAGKAAEGLEDSSGIRSRTLASWELIWADGHETLHRGDVIVIDEAGMVS
SQQMARVLKLVEQAGAKVVLVGDAMQLQPIQAGAAFRAITERIGFAELAGVRRQREDWAREASRLFARGE
IEKGLDAYAQQGHLIEAATRDEIIDRIVADWSEARKLAIETSVLEGNDGRLRGDELLVLAHTNDDVKRLN
EALRSVMTGEGALGEGRSFRAERGAREFAAGDRIIFLENARFLEPRAPRLGPQYVKNGMLGTVVSTGDKR
GDALLSVRLDNGRDVVISEDSYRNVDHGYAATIHKSQGATVERTFVLATGMMDQHLTYVSMTRHRDRADL
YAAKEDFEARPEWGRKPRVDHAAGVTGELVETGQAKFRPNDEDADDSPYADVKTDDGAVHRLWGVSLPKA
LEESGVSQGDTVTLRKDGVERVKVQVPIVDEQTGQKRFEEREVDRNVWTAKRIETAEARLERIERESHRP
DVFKQLVERLSRSGAKTTTLDFESEAGYRAHADDFARRRGLDTLSRAAAGMEEEVSRRWAWIAEKREQVA
ELWERASMALGFAIERERRVSYNEERIETQSVSDADAGRARYLIAPTTVFARSVDEDARLAQLASPTWKE
REAILRPLLEKIYRDPDAALVALNALSSDAGLEPRKLAEDLAAAPDRLGRLRGSDLIVDGRAARDERSVA
TAALLELIPLARAHATEFRRNAERFDLREQQRRAHMSLPIPAPSRKAMKRLVEIEAVRRQGGDDAYKSAF
ALATEDRSLVQEVKAVSEALTARFGWSAFTSKADAVAERNVVERMPEDLASIRGEKLTRLFEAVKRFAEE
QHLVERRDRSKIVAGASADQGMEAGKENAAVLPMLAAVTEFKTPIDEEARLRTLATPLYRQQRAALATVA
SMIWRDPAGAVSKIEELLQKGFAGERIAAAVTNDPAAYGALRGSDRLMDRMLASGRERKEAVQAMPEAAA
RLRALGSAYVNVLDAERQAITEERRRMTVAIPGLSKAAEEALMRLTAEAKNNGRKLSAPAASLDPGIRRE
FDAVSRALDERFGRNAIVRGEKDLINRIPPLQRRAFEALQDRLKVLQQAVRMESSEQIISERRQRAVSRG
RGIDL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 5331 | GenBank | WP_094088886 |
| Name | traG_CHY08_RS32510_pSK04 |
UniProt ID | _ |
| Length | 639 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 639 a.a. Molecular weight: 70527.32 Da Isoelectric Point: 9.2595
>WP_094088886.1 Ti-type conjugative transfer system protein TraG [Rhizobium leguminosarum]
MALKSRLHPSLLLVFVPIAVTAITVYITGWRWTALAHGLSGRTEYWFLRAAPVPALLFGPLAGLLTVWAL
PLHRRRPIAVASLLCFLVVAGFYALREYGRLVPSVERGIVTWDHALSYLDFVAVIGALAGFIAGAAAARI
SVVVSEPVKRAKGGTFGDADWLSMPAAARLFPPDGEIVVGERYRVDKEVVHDLPFDPNDRNTWGQGGKAP
LLTYSQDFDSTHMLFFAGSGGYKTTSNVLPTALRYAGPLICLDPSTEVAPMVLEHRTRALGREVMVLDPT
NPIMGFNVLDGIEASKQKEEDIVGIAHMLLSESLRFESSTGSYFQNQAHNLLTGLLAHVMLSPEYAGRRN
LRSLRQIVSEPEPSVLAMLRDIQEHSESAFIRETLGVFTNMTEQTFSGVYSTASKDTQWLSLDSYAALVC
GNTFRSSDIIAGGTDVFLNIPASILRSYPGIGRVIIGSLINAMVQADGRFSRRALFMLDEVDLLGYIRVL
EEARDRGRKYGISMMLLYQSVGQLERHFGKDGATSWIDGCAFVSYAAIKALDTARNVSAQCGEMTVEVQG
RSRNIGWDTKRGGTRKSESVNFQRRPLIMPHEITQSMRKDEQIIIVQGHRPIRCGRAIYFRRKDMNAAAK
ANRFVKPVP
MALKSRLHPSLLLVFVPIAVTAITVYITGWRWTALAHGLSGRTEYWFLRAAPVPALLFGPLAGLLTVWAL
PLHRRRPIAVASLLCFLVVAGFYALREYGRLVPSVERGIVTWDHALSYLDFVAVIGALAGFIAGAAAARI
SVVVSEPVKRAKGGTFGDADWLSMPAAARLFPPDGEIVVGERYRVDKEVVHDLPFDPNDRNTWGQGGKAP
LLTYSQDFDSTHMLFFAGSGGYKTTSNVLPTALRYAGPLICLDPSTEVAPMVLEHRTRALGREVMVLDPT
NPIMGFNVLDGIEASKQKEEDIVGIAHMLLSESLRFESSTGSYFQNQAHNLLTGLLAHVMLSPEYAGRRN
LRSLRQIVSEPEPSVLAMLRDIQEHSESAFIRETLGVFTNMTEQTFSGVYSTASKDTQWLSLDSYAALVC
GNTFRSSDIIAGGTDVFLNIPASILRSYPGIGRVIIGSLINAMVQADGRFSRRALFMLDEVDLLGYIRVL
EEARDRGRKYGISMMLLYQSVGQLERHFGKDGATSWIDGCAFVSYAAIKALDTARNVSAQCGEMTVEVQG
RSRNIGWDTKRGGTRKSESVNFQRRPLIMPHEITQSMRKDEQIIIVQGHRPIRCGRAIYFRRKDMNAAAK
ANRFVKPVP
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Host bacterium
| ID | 8033 | GenBank | NZ_CP022568 |
| Plasmid name | pSK04 | Incompatibility group | - |
| Plasmid size | 326874 bp | Coordinate of oriT [Strand] | 235127..235155 [-] |
| Host baterium | Rhizobium leguminosarum bv. viciae strain BIHB 1148 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |