Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 107597
• Name: oriT_pSK02
• Organism: Rhizobium leguminosarum bv. viciae strain BIHB 1148
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP022566 (34724..34752 [-], 29 nt)
• oriT length: 29 nt
• IRs (inverted repeats): 14..19, 24..29 (CGTCGC..GCGACG)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 29 nt

>oriT_pSK02
AGGGCGCAATATACGTCGCTGTCGCGACG

oriT secondary structure

Predicted by RNAfold.

Relaxase

traA_CHY08_RS26900_pSK02

• ID: 5107
• GenBank: WP_094088384
• Name: traA_CHY08_RS26900_pSK02
• UniProt ID: _
• Length: 1545 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 1545 a.a.
• Molecular weight: 171110.24 Da
• Isoelectric Point: 6.6888

>WP_094088384.1 Ti-type conjugative transfer relaxase TraA [Rhizobium leguminosarum]
MAIMFVRAQVISRGVGRSIVSAAAYRHRARMMDEQAGTSFSYRGGAPELVHEEVALPDWTPAWLRAAIDG
RTVAAASEALWNAVDVFEKRADAQLARELIIALPEELTRAENIALVREFVRDNLTSKGMVADWVYHDKDG
NPHIHLMTTLRPLTQEGFGRKKVAVTGEDGAPLRVVTPDRPKGRIVYKLWAGDKETLQAWKVAWAETANR
HLALAGHEIRLDGRSYAEQGLDGIAQKHLGPEKAALSRRGAEMYFAPADLARRQEMADRLLADPTLLLKQ
LGNERSTFDERDIAKALHRYVDDPADFANIRARLMASDDLVMLKPQQVDAETGKASEPAVFTTREILRVE
YDMSQSARVLSERRGFAVSAESVAAAIENVETADPEKSFRLDAEQVDAVRHITGDSGIAAVVGLAGAGKS
TLLAAARLAWEHDGRRVIGAALAGKAAEGLEDSSGIRSRTLASWELIWADGHETLHRGDVIVIDEAGMVS
SQQMARVLKLVEQAGAKVVLVGDAMQLQPIQAGAAFRAITERIGFAELAGVRRQREDWAREASRLFARGE
IEKGLDAYAQQGHLIEAATRDEIIDRIVADWSEARKLAIETSVLEGNDGRLRGDELLVLAHTNDDVKRLN
EALRSVMTGEGALGEGRSFWAERGAREFAAGDRIIFLENARFLEPRAARLGPQYVKNGMLGTVVSTGDKR
GEVLLSVRLDNGRDVVIRENSYRNVDHGYAATIHKSQGATVERTFVLATGMMDQHLTYVSMTRHRDRADL
YAAKEDFEARPEWGRKPRVDHAAGVTGELVETGQAKFRPNDEDADDSPYADVKTEDGSVHRLWGVSLPKA
LEEGGVSEGDTVTLRKDGVERVTIKVPVVDEQTGQKRFEEREVDRNVWTAKRIETAEARLERVERESHRP
DVFKQLVERLSRSGAKTTTLDFESEAGYRAHADDFARRRGLDTLSRAAAGMEEEVSRRWAWIAEKREQVA
ELWERASMALGFAIERERRVSYNEERIETQSVSDADAGRARHLIAPTTEFARSAEEDARRAQLSSPAWKE
REAILRPLLEKIYRDPDAALSALNALSSDMSIEPRKLAEDLAAAPDRLGRLRGSDLIVDGRAARDERNAA
ITALTELLPLARAHATEFRQNGERFDLREQQRRAHMSLPIPALSKTAMARLVEIEAVRRQGGDDAYKTAF
ALATEDRSLVQEVKAVSEALAARFGWSAFTSKADAVAERNMVERMPEDLTSTRREKLARLFEAVKRFADE
QHLVERRDRSKIVAAASADQGMEAGKENAAVLPMLAAVTEFKTPVDEEARSRLLSAPLYRQQRAALAAVA
STIWRDPAGAVGKIEELLQKGFAGERIAAAVTNDPAAYGALRGSDRLMDRMLASGRERKEAVQAMPEAAA
RLRALGSAYVNVLDAERQAITEERRRMTVAIPGLSKAAEEALMRLTAEAKNNGRKLSAPAASLDPGIRRE
FDAVSRALDERFGRNAIVRGEKDLINRIPPLQRRAFEAMQDRLKVLQQAVRMESSEQIISERRQRAVNRG
RGIGF

Protein domains

Predicted by InterproScan.

• MobA/MobL family (MobA_MobL) (17-249)

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (1047-1127)

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (1312-1405)

• AAA domain (AAA_30) (391-577)

Protein structure

No available structure.

Host bacterium

• ID: 8032
• GenBank: NZ_CP022566
• Plasmid name: pSK02
• Incompatibility group: -
• Plasmid size: 549819 bp
• Coordinate of oriT [Strand]: 34724..34752 [-]
• Host baterium: Rhizobium leguminosarum bv. viciae strain BIHB 1148

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: actP
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA9