Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   107596
Name   oriT_CCGM7|C in_silico
Organism   Sinorhizobium americanum CCGM7
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP013054 (889159..889187 [+], 29 nt)
oriT length   29 nt
IRs (inverted repeats)      14..19, 24..29  (CGTCGC..GCGACG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 29 nt

>oriT_CCGM7|C
AGGGCGCAATATACGTCGCTGTCGCGACG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   5106 GenBank   WP_037382860
Name   traA_SAMCCGM7_RS25890_CCGM7|C insolico UniProt ID   _
Length   1539 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1539 a.a.        Molecular weight: 170202.28 Da        Isoelectric Point: 9.5559

>WP_037382860.1 Ti-type conjugative transfer relaxase TraA [Sinorhizobium americanum]
MAIMFVRAQVISRGAGRSIVSAAAYRHRARMMDEQAGTSFSYRGGSRELVHEELALPDQVPAWLKTATAG
QSVATASEALWNALDAFETRADAQLARELIIALPEELTRAENIGLVREFVRDSFTSKGMVADWVFHDKDG
NPHIHLMTTLRPLTEEGFGPKKVPVLGEDGEPLRVVTPDRPNGKIIYKLWAGDKEAMKAWKIAWAETANR
HLALAGHEIRLDGRSYAEQGLDGVAQKHLGPERASLARKGAEMYFAPADLARRQEMADRLLNDPELLLKQ
LGNERSTFDEKDIAKALHRYVDDPVYFANIRARLMASDELVMLRPQQLDAETGKASEPAVFTTREILRIE
YDMARSAELLSQRKGFGVPGAEVAAAVQSIETADPAKPFKLEAEQVDAVSHVTGNSGIAAVVGLAGAGKS
TLLAAARVAWEREGRRVTGAALAGKAAEGLEDSSGIRSLTLASWELAWASGRETLHRGDVLVVDEAGMVS
SQQMGRVLKAVEDAGAKAVLVGDAMQLQPIQAGAAFRAISERVGFAELAGVRRQRDAWAREASRLLARGK
VEAGLDAYASRGRLVEAQTREEVIGRIISDWTDARKQAIEKSVSRGNEGRLRGDELLVLAHTNDDARKLN
GAVRKVMEDDGALSGGRAFQTARGAREFAVGDRIMFLENARFLEPRARRLGPQYVKNGMLGAVVSTGDKR
GDTLLSVRLDNGRHVVISEASYRNVDHGYAATIHKSQGSTFDRTFVLATGMMDQHLTYVAMTRHRERADL
YAAKEDFEAKPEWGREARIDHAAGVTGALVEVGMAKFRPDDEDAKESPYANIRTDDGTVHRVWGVSLPKA
IEDAGVCEGDTVTLRKDGVERVKVEVPVVDAETGQKRFEQREFDRNVWTAKQLETAETRQVRIERESHRP
ELFNQLVQRLSRSGAKTTTLDFESEAGYQAHAQDFARRRGIDTLAEVAAGVEEGVSRRLAWIAEKREQVA
KLWERASVALGFAIERERLVSYSEDRTELLSAGIPAGGRYLIPPTTEFARSVDEDARRALLSSPRWKERE
DILQPLLAKIYRDPDAALIHLNALASDAGIEPRTLASDLAKAPERLGRLRGSENLVAGRAARDERDIAMA
AFKELLPLVRAHATEFRRNADRFREREQTRRVYMSLSIPAISKQAMARLVEIEAVRSRGGDDAWKTAFAF
ATEDRSLVQEVKAVSDALTARFGWCAFTRKADAIAERNITERMPEDITDEWRGKLTRLFEAVRRFAEEQH
LAERKDRSKIVAGASVELGKEKMAVLPMLAAVTEFKTPVDDVARPRALAAQHYRHNRAALAETATRIWRD
PAGAVDRIEYLIVKGFAAERIAAAVVNDPAAYGALRGSDRLMDRMLAAGRERKDALQVVPEAAVHLRLLG
AAYVGAFDAERQAVTKERRLMAVAIPGLSPAAEEALRRLVSETKKKGSKLDVAAAALKPAIRQEFAAVSR
ALDERFGRNAILRGEKDVIKRVSPLQRRAFEAMHERLKVLQQTVRMQSSEQILSERRQRTINRARGVTW

  Protein domains


Predicted by InterproScan.

BID domain of Bartonella effector protein (Bep) (Bep_C_terminal)

(1041-1125)

BID domain of Bartonella effector protein (Bep) (Bep_C_terminal)

(1309-1397)

MobA/MobL family (MobA_MobL)

(17-249)

AAA domain (AAA_30)

(391-577)


  Protein structure



No available structure.




T4CP

ID   5329 GenBank   WP_037382855
Name   traG_SAMCCGM7_RS25875_CCGM7|C insolico UniProt ID   _
Length   643 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 643 a.a.        Molecular weight: 70739.48 Da        Isoelectric Point: 9.4275

>WP_037382855.1 Ti-type conjugative transfer system protein TraG [Sinorhizobium americanum]
MTPKAKPDPNLPLVLVPVAITAFAVYVVGWRWSEWAAGLSEKSAYWFLRASPVPTLSLGPLSGLLTVHML
PLHRRRPIAMASLVCFLTVAGYYALREYGRLAPAVESGAIRWDQALTYFDMVAVISAVLGFLAVAVAARI
STVVPDLVKRAKRGTLGDADWLPMSAAGKLFPPDGEIVVGERYRVDKELVHALPFDPNDTTTWGRGGKAP
LLTYGQNFDSTHMLFFAGSGGYKTTSNVIPTALRYSGPLICLDPSTEVAPMVVEHRTRVLGREVIVLDPT
NPIIGFNALDGIETSARQEEDIVGIAHMLLSDSARMESSTGSYFQNQAHNLLTGLLAHVMLSPEYAGRRT
LRSLRQIVSEPEPSVLAMLRDIQENSGSTFIRETLGVFTNMTEQTFSGVYSTASKDTQWLSLDSYAALVC
GSTFKSSDIVTARKDVFLNIPASILRSYPGIGRIVIGSLINAMVRADGGFKRRALFMLDEVDLLGYMRVL
EEARDRGRKYGISMMLMYQSVGQLVRHFGKDGATSWIEGCAFASYAAIKALDTARNVSAQCGEMTVEVKG
SSRNIGWDTKNNGSRKSETVNFQRRPLIMPHEITQSMRKDEQIIIVQGHSPIRCGRAIYFRRKDMNGAAK
ANRFVKARYEASD

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(165-640)

  Protein structure



No available structure.



ID   5330 GenBank   WP_037385045
Name   tcpA_SAMCCGM7_RS30235_CCGM7|C insolico UniProt ID   _
Length   928 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 928 a.a.        Molecular weight: 100061.27 Da        Isoelectric Point: 4.6314

>WP_037385045.1 DNA translocase FtsK [Sinorhizobium americanum]
MRIPRTNFSTAALHDADEADDFEHPYQGSPDGSHAQTTRASAQDHLFEAPDAPRRGAGHEADEMGYAGRA
ARLYGHGSAYAPAQVTASTRDPLPTLAEITGQTDGPSWESHFFLSPNVRFTRTPEREFMKRRAPAADESG
GEVEETGAEVAEPDVVAETAPADAPPATAEADTPAHTPSELLRVLMQQLPSWRPAQGRETALAESALDAE
PVVKEEVSVAEEPEGTPARAVRAVDVAEVILADIEPAPIVTGNESDAHLSYLSDHAFFEFMPLETVAVSQ
VAVEPVKELAKAPVQIATAPKPVALPPVEIRPQPPTAITSMFRVVECRPAATAVVAPGPAPVQIVNRSAE
EAVTAPPAPAPASVEPAESVKAEEDTPDLDVPVTKAAITMPAVVQRSTSTLPPVGRGERQQAGDTYEFPA
KELLQEPPQGQGFFMTQEQLEQNAGLLESVLEDFGVKGEIIHVRPGPVVTLYEFEPAPGVKSSRVIGLAD
DIARSMSALSARVAVVPGRNVIGIELPNATRETVYFRELIESNDFQRTGCKLALCLGKTIGGEPVIAELA
KMPHLLVAGTTGSGKSVAINTMILSLLYRLKPEECRLIMVDPKMLELSVYDGIPHLLTPVVTDPKKAVMA
LKWAVREMEDRYRKMSRLGVRNIDGYNQRAAAAREKGEPILATVQTGFEKGTGEPLFEQQEMDLTPMPYI
VVIVDEMADLMMVAGKEIEGAIQRLAQMARAAGIHLIMATQRPSVDVITGTIKANFPTRISFQVTSKIDS
RTILGEQGAEQLLGQGDMLHMAGGGRIARVHGPFVSDLEVEHVVAHLKTQGRPEYLETVTADEEEPEEDQ
GAVFDKSAIAAEDGNELYEQAVKVVLRDKKCSTSYIQRRLGIGYNRAASLVERMEKDGLVGPANHVGKRE
IIHGNRDHAAKADDGEME

  Protein domains


Predicted by InterproScan.

FtsK/SpoIIIE family (FtsK_SpoIIIE)

(527-745)

Ftsk gamma domain (FtsK_gamma)

(853-912)

FtsK alpha domain (FtsK_alpha)

(418-518)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 858173..867728

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SAMCCGM7_RS25720 (SAMCCGM7_pC0851) 853760..854881 - 1122 WP_037382798 Fic family protein -
SAMCCGM7_RS34490 (SAMCCGM7_pC0852) 855143..855268 + 126 WP_281069543 hypothetical protein -
SAMCCGM7_RS25725 (SAMCCGM7_pC0853) 855286..855477 - 192 Protein_827 type II toxin-antitoxin system VapC family toxin -
SAMCCGM7_RS33295 (SAMCCGM7_pC0855) 855925..856095 + 171 WP_156878017 hypothetical protein -
SAMCCGM7_RS25730 (SAMCCGM7_pC0857) 856569..856808 - 240 WP_037382800 hypothetical protein -
SAMCCGM7_RS25735 (SAMCCGM7_pC0858) 857163..857567 - 405 WP_037382802 MarR family transcriptional regulator -
SAMCCGM7_RS34310 857664..858173 + 510 WP_072597423 hypothetical protein -
SAMCCGM7_RS25745 (SAMCCGM7_pC0859) 858173..858835 + 663 WP_037382804 lytic transglycosylase domain-containing protein virB1
SAMCCGM7_RS25750 (SAMCCGM7_pC0860) 858832..859134 + 303 WP_052035467 TrbC/VirB2 family protein virB2
SAMCCGM7_RS25755 (SAMCCGM7_pC0861) 859139..859477 + 339 WP_037382805 type IV secretion system protein VirB3 virB3
SAMCCGM7_RS25760 (SAMCCGM7_pC0862) 859470..861836 + 2367 WP_037382806 VirB4 family type IV secretion system protein virb4
SAMCCGM7_RS25765 (SAMCCGM7_pC0863) 861836..862531 + 696 WP_037382808 P-type DNA transfer protein VirB5 virB5
SAMCCGM7_RS25770 (SAMCCGM7_pC0864) 862528..862761 + 234 WP_037382810 EexN family lipoprotein -
SAMCCGM7_RS25775 (SAMCCGM7_pC0865) 862767..863720 + 954 WP_037382812 type IV secretion system protein virB6
SAMCCGM7_RS25785 (SAMCCGM7_pC0866) 864011..864682 + 672 WP_037382814 virB8 family protein virB8
SAMCCGM7_RS25790 (SAMCCGM7_pC0867) 864694..865542 + 849 WP_156878051 P-type conjugative transfer protein VirB9 virB9
SAMCCGM7_RS25795 (SAMCCGM7_pC0868) 865551..866726 + 1176 WP_037382818 type IV secretion system protein VirB10 virB10
SAMCCGM7_RS25800 (SAMCCGM7_pC0869) 866739..867728 + 990 WP_037382819 P-type DNA transfer ATPase VirB11 virB11
SAMCCGM7_RS25805 867803..868090 - 288 WP_037382820 hypothetical protein -
SAMCCGM7_RS25810 (SAMCCGM7_pC0871) 868650..870467 + 1818 WP_072597425 ABC transporter ATP-binding protein -
SAMCCGM7_RS25815 (SAMCCGM7_pC0872) 870811..872067 + 1257 WP_037382840 hypothetical protein -

Host bacterium

ID   8031 GenBank   NZ_CP013054
Plasmid name   CCGM7|C Incompatibility group   -
Plasmid size   2249899 bp Coordinate of oriT [Strand]   889159..889187 [+]
Host baterium   Sinorhizobium americanum CCGM7

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   aioR/aoxR, aioS/aoxS, aioX/aoxX, arsC, actP
Degradation gene   benA, benB, benC
Symbiosis gene   -
Anti-CRISPR   AcrIIA7