Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 107593
• Name: oriT_pPR2
• Organism: Rhizobium leguminosarum bv. viciae strain BIHB 1217
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP022667 (377119..377147 [-], 29 nt)
• oriT length: 29 nt
• IRs (inverted repeats): 14..19, 24..29 (CGTCGC..GCGACG)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 29 nt

>oriT_pPR2
AGGGCGCAATATACGTCGCTGTCGCGACG

oriT secondary structure

Predicted by RNAfold.

Relaxase

traA_CHR56_RS32730_pPR2

• ID: 5103
• GenBank: WP_094231098
• Name: traA_CHR56_RS32730_pPR2
• UniProt ID: _
• Length: 1538 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 1538 a.a.
• Molecular weight: 169816.92 Da
• Isoelectric Point: 7.3546

>WP_094231098.1 Ti-type conjugative transfer relaxase TraA [Rhizobium leguminosarum]
MAIMFVRAQVIGRGAGRSVVSAAAYRHRARMMDEQAGTSFSYRGGASELVHEELALPDQIPAWLRSAISG
QSVAKASEALWNAVDAFEKRADAQLARELIIALPEELTRAENIALVREFVLDNLTAKGMVADWVYHDKDG
NPHIHLMTTLRPLAEEGFGPKKVPVCGEDGKPLRVVTPDRPNGKVVYKLWAGDKETLQAWKIAWAETASR
HLALAGHEIRLDGRSYAEQGLDGIAQKHLGPEKAALSRRDAEMYFAPADLARRQEMADRLLADPALLLKQ
LSNERSTFDERDIAKALHRTVDDPADFANIRARLMASDDLIMLKPQQIDAETGKASEPAVFTTREMLRIE
YDMGRSARVLAERRGFAVSDRVVAAAIRQVETQDPENSFRLDPEQADAIHHITRDKGIAAVVGLAGAGKS
TLLAAARVAWESDGRRVIGAALAGKAAEGLEDSSGIRSRTLAAWELAWASGRELLERGNVLVIDEAGMVS
SQQMARLLDIAEQAEVKVVLVGDAMQLQPIQAGAAFRAITERIGFAELAGVRRQRQPWAREASRLFARGE
TGKGLDAYAQQGHLIEAGTRAETIDRIVADWSQARKQAIQNSTTAGNDGRLRGDELLVLAHTNEDVKRLN
EALRSVMVGEGALGEDRAFRTERGAREFAAGDRIIFLENARFVEARAMRLGPQHVKNGMLGTVVSTGDKR
GDAVLSVRLDSGRDVVISESSYRNVDHGYAATIHKSQGATVERSFVLATGMMDQHLTYVSMTRHRDRVDL
YAAKDDFEAKPEWGRKPHVDHAAGVTGALVETGQAKFRPNDEDADDSPYADVRADDGTLHRLWGVSLPKA
LEEGGVSEGDTVTLRKDGVERVTIKVAIVDEQTGQKRFEEREVDRNVWTAKRIETAEARQQRIERESHRP
DVFKQLVERLSRSGAKTTTLDFESEAGYRAHADDFARRRGLDHLSLAAAGAEESLSRRWAGIAERREQLA
KLWERASVALGFTIERERRIAYNEERAEPQTVLEADVARGRYLIPPTTGFARSAEEDAQLAQLSSPAWKE
REAILRPLLEKIYRDPDAALVALNALSSDTSVDPRRLADDLAKAPERLGRLRGSELIVDGRAARDERNAA
ITALTDLLPLVRAHATQFRRNAERFESHEQQRRAHMALPIPALSEKAMARLTEIEAVRRQGGDDAYKAAF
ALAAEDRSIVQEVKAVSEALTARFGWRAFTSKADAVGERNMVERMPEDLASIRGEKLTRLFKAVKRFADE
QHLAERRDRSKIVAGASADQGMENVPMLAAVTEFRTAIDEEARSRVLSDPLYRQQRAALAAVASAIWRDP
VGAVGKIEDLLQKGFAGERIAAAVTNDPAAYGALRGSDRLMDRMLAVGRERKEAVQAVPEAAARLRSLGS
AYVNVLDAERQAIAEERRRMAVAIPALSTAAEDVLMRLTAEAKNNDRKLTASAAPLDPSIRREFAAVSRA
LDERFGRNAIIRGEKDLINRVPQAQRSAFEAMQDRLKVLQRTVRMESSEQIISERRQRAVSRGRGIEL

Protein domains

Predicted by InterproScan.

• MobA/MobL family (MobA_MobL) (17-249)

• AAA domain (AAA_30) (391-576)

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (1305-1399)

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (1047-1127)

Protein structure

No available structure.

Host bacterium

• ID: 8028
• GenBank: NZ_CP022667
• Plasmid name: pPR2
• Incompatibility group: -
• Plasmid size: 605219 bp
• Coordinate of oriT [Strand]: 377119..377147 [-]
• Host baterium: Rhizobium leguminosarum bv. viciae strain BIHB 1217

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: actP
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA9