Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   107580
Name   oriT_pRetTAL182c in_silico
Organism   Rhizobium sp. TAL182
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP021027 (201990..202018 [+], 29 nt)
oriT length   29 nt
IRs (inverted repeats)      14..19, 24..29  (CGTCGC..GCGACG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 29 nt

>oriT_pRetTAL182c
AGGGCGCAATATACGTCGCTGTCGCGACG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   5094 GenBank   WP_085861369
Name   traA_Tal182_RS25415_pRetTAL182c insolico UniProt ID   _
Length   1562 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1562 a.a.        Molecular weight: 172386.71 Da        Isoelectric Point: 8.0075

>WP_085861369.1 MULTISPECIES: Ti-type conjugative transfer relaxase TraA [Rhizobium]
MAIMFVRAQVISRGSGRSIVSAAAYRHRARMMDEQAGTSFSYRGGAGELMYEELALPDEIPDWLRSAISG
QSVSKASEVFWNAVDAFETRADAQLARELIIALPEELTRAENITLVREFVRDNLTSKGMIADWVYHDKDG
NPHIHLMTTLRPLTEEGFGAKKVPVLGEGGKPLRVVTPDRPNGKIVYKVWAGDKETMKAWKIAWAETANR
HLALAGHDIRLDGRSYAEQGLDGIAQKHLGPEKAALARKGRELHFAPADLARRQEMADRLLSEPELLLKQ
LGNERSTFDERDIARALHRYVDDPTDFANIRARLMASDQLVILKPQEIEAETGKVSEPAVFTTREMLRIE
YDMAQSARVLSERRGFGVSERNVTVAIERVESGDPKNPFRLDAEQVDAVRHVTGDGGIAAIVGLAGAGKS
TLLAAARLAWESEGHRVIGAALAGKAAEGLQDSSGIKSRTLASWELAWANGRDTLHRGDVLVIDEAGMVA
SQQMARVLKIAEEAEVKVVLVGDAMQLQPIQAGAAFRAITERIGFAELAGVRRQREAWARNASRLFARGE
VEKGLDAYARHGHLVEAGSREETIDRIVSDWAAARREAIERSTSEGGDGRLRGDELLVLAHTNDDVRKLN
EALRSVMTQEGALGESRSFRSERGAREFAAGDRIIFLENARFLEPRAKHSGPQYVKNGMLGTVVSTGDKR
GDPLLSILLDNGRKLVFSEDSYRHVDHGYAATIHKSQGATVDRTFVLATGMMDQHLTYVSMTRHRDRVDL
YAAKEDFAAKPEWGRKPRVDHAAGVTGELVETGQAKFRPDDEDADDSPYADVRADDGTVHRLWGVSLPKA
LEEAGIQEGDTVTLRKDGVERVKVQIAVVDEKTGHKHYEEREVDRNVWTASQVETASARQERIARESHRP
ELFNPLVERLSRSGAKTTTLDFESEASYRAHANDFARRRGLDHLSLAAAEMEQSLTRRWAWIAAKREQVE
KLWERASVALGFAIERERRVVYNEERSQTMVEATSSDARTASHSVSGASAAETRYLIPPATSFVSSVEED
ARLAQLASPAWTEREVILRPLLQKIYRDPDAALVSLNALASDIGVAPRRLADDLAAAPGRLGRLRGSELI
VDGGAAREERNLAVAAVKELLPMARAHATEFRRNAERFVLREQTRRAHMSLSIPALSERAMARLMEIEAV
RRQGGDDAYKTAFALTAKDRSVVQEIKAVSEALTARFGWSAFSAKADAIAERNIVERMPEDLTDERRGKL
TRLFDAVRRFADEQHLAERRDRSKVVAGASVVRGQETENGPGKENVAVPPMLAAVIAFKTSVDDEARLRA
LSNPFYRQQRGALANAATTIWRDPAEVVGKIEELLQKGFAAERIGAAVTNNPAAYGALRGSDRLMDRMLT
SGRERKEAVAAVPEAAARLRALGAAHLNALDAERQAITDERRRMAVAIPALSKAAEEALAHLTVEVSKDS
RKLSVSAASLDPGIGREFAAVSRALDERFGRNALVRGDKDLVNRVPPAQRRAFEAMQERLKVLQQTVRLQ
SSEQIIAERRQRAASRARGINL

  Protein domains


Predicted by InterproScan.

MobA/MobL family (MobA_MobL)

(17-249)

AAA domain (AAA_30)

(391-576)

BID domain of Bartonella effector protein (Bep) (Bep_C_terminal)

(1069-1140)

BID domain of Bartonella effector protein (Bep) (Bep_C_terminal)

(1332-1422)


  Protein structure



No available structure.




T4CP

ID   5313 GenBank   WP_008536019
Name   traG_Tal182_RS25400_pRetTAL182c insolico UniProt ID   _
Length   639 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 639 a.a.        Molecular weight: 70668.81 Da        Isoelectric Point: 9.2416

>WP_008536019.1 MULTISPECIES: Ti-type conjugative transfer system protein TraG [Rhizobium]
MGLRGKPHPSLLLVLVPIAVTTITIYIVGWRWPGLAAGMSGKIEHWFLRAAPVPPLLFGPLAGLLTVWAL
PLHRRRPVAMASLLYFLGVAAFYALREFGRLAPAVQAEVITWDRALSYLDMVAVIAAVAGFMAVAMSARI
SVVVPDEIKRARRGIFGDADWLPMTAAGKLFPPDGEIVVGERYRVDKEIIHALPFDVNDRSTWGQGGKAP
LLTYRQDFDSTHMLFFAGSGGYKTTSNVVPTALRYTGPMICLDPSTEVAPMVVEHRTNALGREVMVLDPT
NPIMGFNVLDGIEASKQKEEDIVGIAHMLLSESLRFESSTGSYFQNQAHNLLTGLLAHVMLSPEYEGRRS
LRSLRQIVSEPEPSVLAMLRDIQEHSGSAFIRETLGVFTNMTEQTFSGVYSTASKDTQWLSLDSYAALVC
GNAFKSGDIVSGKKDVFLNISASILRSYPGIARVIIGSLINAMVQADGAFQRRALFMLDEVDLLGYMRVL
EEARDRGRKYGISMMLMYQSVGQLERHFGKDGATSWIDGCAFASYAAIKALDTARNLSAQCGEMTVEVKG
SSRNIGWDTKNNASRRSENVNFQRRPLIMPHEITQSMRKDEQIIIVQGHSPIRCGRAIYFRRKEMDQAAK
VNRFVKPVL

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(170-634)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 181442..191029

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Tal182_RS25255 (TAL182_PC00199) 178234..178443 - 210 Protein_173 chaperonin GroEL -
Tal182_RS25260 (TAL182_PC00200) 178738..179657 + 920 Protein_174 zincin-like metallopeptidase domain-containing protein -
Tal182_RS25270 (TAL182_PC00202) 180432..180803 - 372 WP_037145471 hypothetical protein -
Tal182_RS25275 (TAL182_PC00203) 180902..181438 + 537 WP_018446357 hypothetical protein -
Tal182_RS25280 (TAL182_PC00204) 181442..182101 + 660 WP_037145469 transglycosylase SLT domain-containing protein virB1
Tal182_RS25285 (TAL182_PC00205) 182098..182397 + 300 WP_007636189 TrbC/VirB2 family protein virB2
Tal182_RS25290 (TAL182_PC00206) 182403..182741 + 339 WP_037145466 type IV secretion system protein VirB3 virB3
Tal182_RS25295 (TAL182_PC00207) 182734..185100 + 2367 WP_085861367 VirB4 family type IV secretion system protein virb4
Tal182_RS25300 (TAL182_PC00208) 185097..185798 + 702 WP_085861368 P-type DNA transfer protein VirB5 virB5
Tal182_RS25305 (TAL182_PC00209) 185795..186028 + 234 WP_008537008 EexN family lipoprotein -
Tal182_RS25310 (TAL182_PC00210) 186031..186963 + 933 WP_037146965 type IV secretion system protein virB6
Tal182_RS32920 (TAL182_PC00211) 187003..187284 + 282 WP_080712063 hypothetical protein -
Tal182_RS25320 (TAL182_PC00212) 187286..187957 + 672 WP_008535994 virB8 family protein virB8
Tal182_RS25325 (TAL182_PC00213) 187954..188811 + 858 WP_037146970 P-type conjugative transfer protein VirB9 virB9
Tal182_RS25330 (TAL182_PC00214) 188820..189992 + 1173 WP_037146971 type IV secretion system protein VirB10 virB10
Tal182_RS25335 (TAL182_PC00215) 190001..191029 + 1029 WP_037146974 P-type DNA transfer ATPase VirB11 virB11
Tal182_RS25340 (TAL182_PC00216) 191073..191651 - 579 WP_008536010 PIN domain-containing protein -
Tal182_RS25345 (TAL182_PC00217) 191641..192087 - 447 WP_244597434 DNA-binding protein -
Tal182_RS25350 (TAL182_PC00219) 192600..193790 - 1191 WP_037146978 DUF1173 domain-containing protein -
Tal182_RS32925 194100..194357 + 258 Protein_192 toprim domain-containing protein -
Tal182_RS25355 (TAL182_PC00221) 194595..195457 + 863 Protein_193 DUF2493 domain-containing protein -
Tal182_RS25360 (TAL182_PC00222) 195542..195808 + 267 WP_004676093 hypothetical protein -

Host bacterium

ID   8015 GenBank   NZ_CP021027
Plasmid name   pRetTAL182c Incompatibility group   -
Plasmid size   442228 bp Coordinate of oriT [Strand]   201990..202018 [+]
Host baterium   Rhizobium sp. TAL182

Cargo genes

Drug resistance gene   -
Virulence gene   hsiC1/vipB
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   nifX, nifK, nifD, nifH, nifS, nifW, fixA, fixB, fixC, fixX, nifA, nifB, nifZ, nifT, a9174_31165, nodD, nodJ, nodI, nodS, nodC, nodB, nodZ, fixG, fixO, fixN, mLTONO_5203, nifN, nifE, nodA
Anti-CRISPR   -