Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 107573
• Name: oriT1_pGA2000
• Organism: Streptococcus pyogenes
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NC_019252 (1010..1047 [-], 38 nt)
• oriT length: 38 nt
• IRs (inverted repeats): _
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 38 nt

>oriT1_pGA2000
CACATTTACGAAGTAAAGTATAATGCGTTATACTTTAC

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobV_HS755_RS00030_pGA2000

• ID: 5087
• GenBank: WP_015060459
• Name: mobV_HS755_RS00030_pGA2000
• UniProt ID: G3ER47
• Length: 500 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 500 a.a.
• Molecular weight: 58493.84 Da
• Isoelectric Point: 6.0256

>WP_015060459.1 MobV family relaxase [Streptococcus pyogenes]
MSYLVARMQKMKVGNLGGAYRHNERVFENHSNKDIDTSRSHLNYELMDRDRSVSYEKQIKDYVNEKKSSK
RAIRKDAVLCDEWIITSDKDFFERLDQEQTRAFFETAKNYFAERYGLENIAYASVHLDESTPHMHMGVVP
MVDGKLSSKAVFTREELKAIQEELPKYMNEQGFELSRGQLGSDKKHLSVADYKAKIGKEALNKELLGLGA
PRYWHKEEDRPATAEEIAGYESLASLFAGEEMKLREATLEERFTWLDSHRNDLKGDLSHLEELVDKKIEE
YTRIDSEASERLSELSELNSKVKDREKELRGLESDSERLSDKVVRLEKEHRETTQLLVEQNRNLRKISFQ
DLDRRRIAEDLHEELEKATPKLFGGSFNFTADFVGRLKTFMSEVVEKLEQVINQNEVLRKALEGMKQAKE
RAERELLQEEWKTQRLETENQNLRQENKELKVSKNLLEDIQEVITEKEVSSLNKRLDELREARMASKRGY
EPKRSKGRSI

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-194)

Protein structure

• AlphaFold DB: G3ER47

Host bacterium

• ID: 8008
• GenBank: NC_019252
• Plasmid name: pGA2000
• Incompatibility group: -
• Plasmid size: 4967 bp
• Coordinate of oriT [Strand]: 1010..1047 [-]; 4648..4688 [-]
• Host baterium: Streptococcus pyogenes

Cargo genes

• Drug resistance gene: erm(T)
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -