Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 107572
• Name: oriT1_pRW35
• Organism: Streptococcus pyogenes
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NC_010423 (899..936 [-], 38 nt)
• oriT length: 38 nt
• IRs (inverted repeats): _
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 38 nt

>oriT1_pRW35
CACATTTACGAAGTAAAGTATAGTACGTTATACTTTAC

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobV_HS462_RS00025_pRW35

• ID: 5086
• GenBank: WP_012301163
• Name: mobV_HS462_RS00025_pRW35
• UniProt ID: A8W663
• Length: 500 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 500 a.a.
• Molecular weight: 58462.81 Da
• Isoelectric Point: 6.2804

>WP_012301163.1 MobV family relaxase [Streptococcus pyogenes]
MSYLVARMQKMKVGNLGGAYRHNERVFENHSNKDIDTSRSHLNYELTDRDRSVSYEKQIKDYVNEKKSSK
RAIRKDAVLCDEWIITSDKDFFERLDQEQTRAFFETAKNYFAERYGLENIAYASVHLDESTPHMHMGVVP
MVDGKLSSKAVFTREELKAIQEELPKYMNEQGFELSRGQLGSDKKHLSVADYKAKIGKEALNKELLGLGA
PRYWHKEEDRPATAEEIAGYESLASLFAGEEMKLREATLEERFTWLDSHRNDLKGDLSHLEELVDKKIEE
YTRIDSEASERLSKLSELNSKVKDREKELRGLESDSERLSDKVVRLEKEHRETTQLLVEQNRNLRKISFQ
DLDRRRIAEDLHEELEKATPKLFGGSFNFTADFVGRLKTFMSEVVEKLEQVINQNEVLRKALEGMKQAKE
RAERELLQEEWKTQRLETENQNLRQENKELKVSKNLLEDIQEVITEKEVSSLNKRLDELREARMASKRGY
EPKRSKGRSI

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-194)

Protein structure

• AlphaFold DB: A8W663

Host bacterium

• ID: 8007
• GenBank: NC_010423
• Plasmid name: pRW35
• Incompatibility group: -
• Plasmid size: 4968 bp
• Coordinate of oriT [Strand]: 899..936 [-]; 4537..4577 [-]
• Host baterium: Streptococcus pyogenes

Cargo genes

• Drug resistance gene: erm(T)
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -