Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 107551
• Name: oriT_pHDC-I
• Organism: Tetragenococcus halophilus
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NC_015257 (2845..2864 [-], 20 nt)
• oriT length: 20 nt
• IRs (inverted repeats): _
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 20 nt

>oriT_pHDC-I
GTGGGGTGTAAGTGCGCATT

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobQ_HXC36_RS00025_pHDC-I

• ID: 5065
• GenBank: WP_013650706
• Name: mobQ_HXC36_RS00025_pHDC-I
• UniProt ID: F1SZS0
• Length: 683 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 683 a.a.
• Molecular weight: 80523.36 Da
• Isoelectric Point: 8.9922

>WP_013650706.1 MobQ family relaxase [Tetragenococcus halophilus]
MAIFHMNFTNISASDGRSAVASASYRSGEKLYNEQEGKTYFYPRNVQPDAFILTPEHAPEWTKNRQKLWN
EVEAKDRKANSRYAKEFNVALPVELSHEEQKSLLKHYVQENFVDQGMVADIAIHRDKQENPHAHVMLTNR
PFNLDGTWGVKSKKEYLLDDQGNKTYTGNSKYPKSRKILTTDWDKKEKIAEWRHNWAETVNQKLEEKKLP
DRISEKSYQDQGIGKQATIHEGYHRGKNERKNYNKTLKEIESAKAEKENIQEKINHKEQFNALKDHLSFE
EKHIVSNLSKELKTYINLENIENKERMLFHWKNSLFIKHAVGEDVAQELQTVNRQEYSMEEANHLLNKVT
DRTVQKIYPNVNFNQTTQAERRELIKESESENRIFEGNELDDRLTSIRENLLDQQITVFTKRPYASWKIL
EKQGEKAQKIIDKTLAKYNETMEDMKNIEKGHFSKYDNQDRKAITDNTKNLMTINKIKNIVQEHYDMVLE
KSLPNADFYNINMAEKEQFYSAIVYYNSELKPLDINQINKLKEETPQMFNSIEHRKGLNYLNGHLEAKDL
GNEKLKNVLKHDGTRQLFLSECKKDNHISSDQVENVKQNLKQQQQKQDNYRKQVMTDYEPVNYKEVSNEE
YLQKVLSNTMMSLLYASGRSNVDKKQRKDTEWEMTKKQRENQKGRSSSNGMHL

Protein domains

Predicted by InterproScan.

• MobA/MobL family (MobA_MobL) (17-241)

• Nicking enzyme C-terminal middle helical domain (NES_C_h) (276-384)

Protein structure

• AlphaFold DB: F1SZS0

Host bacterium

• ID: 7986
• GenBank: NC_015257
• Plasmid name: pHDC-I
• Incompatibility group: -
• Plasmid size: 23071 bp
• Coordinate of oriT [Strand]: 2845..2864 [-]
• Host baterium: Tetragenococcus halophilus

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA21