Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   107540
Name   oriT_p21801|I in_silico
Organism   Yersinia pestis isolate p21801
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_LT844544 (47116..47400 [+], 285 nt)
oriT length   285 nt
IRs (inverted repeats)      184..189, 191..196  (AAAAGT..ACTTTT)
Location of nic site      109..110
Conserved sequence flanking the
  nic site    		  TTTGGTTAAA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 285 nt

>oriT_p21801|I
GGTTATTGCTACTTAATGCCGATAACGACTCAGGCTTTGAGGTTTTTTTATACGGTTCACATTTCGTTAGCAAGGCCAGGGTTTTTTGATAAAATTCTGGTTAGTTTGGTTAAAAAGTGTTACAAGTAAGGGTAATGGCTGAAAGGTTAGTTTTAAGGTTCAAAGCGGCAGTATTAAAATTCCAAAAGTTACTTTTCATCCTTCAGAATCCAGACCTTAATTTCATGTAGAAGATTCGTACAATTGTATTGGCGCAAGGACAATCCGCACATGTCAGAATCAGAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   5058 GenBank   WP_001011149
Name   mobH_P2180_RS00315_p21801|I insolico UniProt ID   A0A0G3BA46
Length   1011 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1011 a.a.        Molecular weight: 113023.00 Da        Isoelectric Point: 4.5108

>WP_001011149.1 MULTISPECIES: MobH family relaxase [Enterobacterales]
MNFRALFLSMQRVFGIFSRRENDVSELMMKDAANFSPFAQIIGEQKYTVPDHPNPEVLKFIEYPTRPAGI
QTFNEQSILSLYRDKLHSISMMLAISDGDIREDAYTFTNLVLKPLIEYIRWIHLLPASENHHHNGIGGLL
SHSLEVAMISLKNANHSELRPIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLSETLTWAPS
SQSLLDWARENNVVEYEIHWRKRIHNQHNIWSSVFLERILDPVCMSFLDRVKKERVYAKMVTALNVYNDG
NDFLSKCVRTSDYYSTGTDLNVLRDPIMGLRSNDAAARAIGTIKHNFTSININNYKTKPMHIIIVNGEVY
LNENAFLDFVLSDFAAHKFNFPQGDAGKTVLVESLVQRGYVEPYDDERVVHYFIPGTYSENEIASIFRNG
IGKLEFYNLLKLRWIGLIFDSYKIPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRESVTRVTDTVN
DAIENTPQYGLQLVNGPDADSNNIISSENTESITDSLEESGADISNEIFETQVVTAIDTAETVNADEPEQ
VEEHDDRSQIHLVEQLHEMLLSAPLPHHAVINIDSVPYLDLDAAIALIPGIDEAAFCNGPFFQLTYRDGS
LDGMWIVRDVNNLRLIQLGDNCAGMQVSTSEPRNTSSLKSLFDTSMYQPLDIPEAPSVNEAASPPQTPLE
LPQPRLNAPVAEEASSVAEQTNAHSEPDSVIATEYEQYGHLLEETLDSDGEAYSDLIASDSTEAEYPATD
PQSSDFAQLPRETALSVAPGDLDYSEGAIKPPAPDATGKETILTSPEPAEDVRETVAAVEKASHLSPALA
RLFAVSTHAEKKHEKTQEPSPVKEVKNPTSSTTVKAPISIEPPGAEEKEAVEEFTLLNDGEVTELEYVEI
ATMLHQILTKLSGSFKRKRKNRFMVLTQNTFYLTQSCIEKYGTQLNAPELFNQLPQYQVTSGAVVNTKCI
AFNIPTLVAASDRAKVDIELIINKLKEVGNL

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(106-301)


  Protein structure


Source ID Structure
AlphaFold DB A0A0G3BA46


T4CP

ID   5275 GenBank   WP_000167418
Name   traD_P2180_RS00310_p21801|I insolico UniProt ID   _
Length   694 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 694 a.a.        Molecular weight: 77969.74 Da        Isoelectric Point: 8.7275

>WP_000167418.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Enterobacterales]
MTKSKRTNLHAQENFYRPILEYRSASVLLICAVIMLVMGFRSDGVNIAPIILYTAVFLLLVCLYRCKTAH
PYLMAHWRVFQRQIMFISLKSLRTINKSNFFSNERKYRQLVQEYKKNNRPVPDRKTYFCNGFEWGPEHAD
RAYQIANLSSDKREIALPFVLSPIARHFETMARSMGGNNAIFAVDRRAPIFVTEDNWFGHTLITGNVGTG
KTVLQRLLSISMLHLGHVIVVIDPKNDAEWRQSLMDEASELGLPFYKFHPAQPSSSVCIDVCNAYTNVSD
LTSRLLSLVSVPGEVNPFVQYAEALVSTVITGLSYTDKKPSIYLIHKNMKSHMSVVNLTIKVMECCFARH
YGPDVWMEKVKYASNDTLQVRFKRLTEWFNAHFLNYEGAEPIEWIDTVGRLVDYSMSDPEHMSKMTAGIM
PLFSRLTEQPLNELLSPSPNTLTSREIVTSDGMFSTGGVLYISLDGLSNPESARAISQLIMSDLTSCAGS
RYNADDGDMSSHSRISIFVDEAHSAINNSMINLLAQGRAAQIALFICTQTISDFIAAANAETANRITGLC
NNYISLRVNDTPTQTLVVENFGKSPISTNMVTYTTGSETTLPHNNFSGSISERKQTTLEESIPKELLGQV
PKFHIVARLQDGRKVVGQIPIAVSEKAMKPNTTLLEMFLKPAGKVTLRQNVGLSYLNKYLRKLH

  Protein domains


Predicted by InterproScan.

AAA-like domain (AAA_10)

(199-245)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(410-638)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 146858..156059

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P2180_RS00850 (P2180_176) 142690..143943 - 1254 WP_001048869 ParA family protein -
P2180_RS00855 (P2180_181) 146858..149539 - 2682 WP_001255540 IncHI-type conjugal transfer ATPase TrhC virb4
P2180_RS00860 (P2180_182) 149548..150498 - 951 WP_001022266 TraV family lipoprotein traV
P2180_RS00865 (P2180_183) 150508..151260 - 753 WP_010892304 protein-disulfide isomerase HtdT -
P2180_RS00870 (P2180_184) 151366..151878 - 513 WP_000429933 hypothetical protein -
P2180_RS00875 (P2180_185) 151887..153245 - 1359 WP_000351840 IncHI-type conjugal transfer protein TrhB traB
P2180_RS00880 (P2180_186) 153235..153675 - 441 WP_001224093 plasmid transfer protein HtdO -
P2180_RS00885 (P2180_187) 153677..154909 - 1233 WP_000202110 type-F conjugative transfer system secretin TraK traK
P2180_RS00890 (P2180_188) 154912..155709 - 798 WP_011011069 TraE/TraK family type IV conjugative transfer system protein traE
P2180_RS00895 (P2180_189) 155709..156059 - 351 WP_001395471 type IV conjugative transfer system protein TraL traL
P2180_RS00900 (P2180_190) 156078..156431 - 354 WP_000424120 pili assembly chaperone -
P2180_RS00905 156913..157338 + 426 WP_154166887 hypothetical protein -
P2180_RS00910 (P2180_192) 157771..158658 - 888 WP_159372764 RepB family plasmid replication initiator protein -
P2180_RS00915 (P2180_193) 159193..160269 + 1077 WP_001015069 hypothetical protein -
P2180_RS00920 (P2180_194) 160287..160487 + 201 WP_000357613 hypothetical protein -

Host bacterium

ID   7975 GenBank   NZ_LT844544
Plasmid name   p21801|I Incompatibility group   IncHI1B
Plasmid size   171435 bp Coordinate of oriT [Strand]   47116..47400 [+]
Host baterium   Yersinia pestis isolate p21801

Cargo genes

Drug resistance gene   tet(B)
Virulence gene   -
Metal resistance gene   corA
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -