Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 107534
• Name: oriT_p132R
• Organism: Staphylococcus epidermidis isolate 132R
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_KX712121 (4097..4215 [+], 119 nt)
• oriT length: 119 nt
• IRs (inverted repeats): 51..58, 60..67 (TTGGGGAT..ATCCCCAA) 21..28, 33..40 (TTTTTTGC..GCAAAAAA) 1..6, 12..17 (TGGCTA..TAGCCA)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 119 nt

>oriT_p132R
TGGCTAGCAATTAGCCACTCTTTTTTGCGTAAGCAAAAAACATAAGGGGCTTGGGGATAATCCCCAACAAGCTGGCGCGTCTGCCACGTTAGTGGCTAGCAAAGCCAATGCTTGCCAAA

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_HTJ32_RS00030_p132R

• ID: 5057
• GenBank: WP_002498904
• Name: Relaxase_HTJ32_RS00030_p132R
• UniProt ID: A0A0H2VJA7
• Length: 336 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 336 a.a.
• Molecular weight: 38414.55 Da
• Isoelectric Point: 9.8063

>WP_002498904.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Bacillales]
MATTKLSATKSTSRAINYAEKRAVEKSGLNCDVDYAKSSFKAARELYGKTDGNQGHVIIQSFQPGEVTPE
QCNQLGLALAEKLVPNHQVAVYTHADTNHVHNHIVLNSIDLNTGKKFNNNKQALRDVRAFNDEVCRAQGL
SVPEKDTARLRYTQTEKAIADPNTTSTAQYSWKDDIREAIDQSTATNMEEFQKHLNQHGITIERVTPKSI
TYRHLAENKKVRGRKLGEDYNKGGIQHGFERQIQRRQQQKHKSDTNFQPKRSTSNRHESTERDTGVTQSD
WDQFAQDTNELERRRQAAESARLVDEKARRDREKRVREKPTSRPIINIDRDHGLEL

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (9-242)

Protein structure

• AlphaFold DB: A0A0H2VJA7

Host bacterium

• ID: 7969
• GenBank: NZ_KX712121
• Plasmid name: p132R
• Incompatibility group: -
• Plasmid size: 7209 bp
• Coordinate of oriT [Strand]: 4097..4215 [+]
• Host baterium: Staphylococcus epidermidis isolate 132R

Cargo genes

• Drug resistance gene: vga(A)
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -