Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 107509 |
| Name | oriT_pUIH-1  |
| Organism | Shigella sonnei strain UIH-1 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_LC477293 (18155..18207 [-], 53 nt) |
| oriT length | 53 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 53 nt
>oriT_pUIH-1
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 5243 | GenBank | WP_000338974 |
| Name | t4cp2_HXH17_RS00280_pUIH-1 |
UniProt ID | _ |
| Length | 652 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 652 a.a. Molecular weight: 73347.89 Da Isoelectric Point: 9.1391
>WP_000338974.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MDAKKTGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQECFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LEQKAKGHNVPDVLVSISAILKTSVPDGGKDLAAWMGQEIENRSWISDKTKSFFFKFMSAPDRTRGSIET
NFSSPLSIFSNPITAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
MDAKKTGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQECFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LEQKAKGHNVPDVLVSISAILKTSVPDGGKDLAAWMGQEIENRSWISDKTKSFFFKFMSAPDRTRGSIET
NFSSPLSIFSNPITAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 34029..57574
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HXH17_RS00210 | 29354..30007 | - | 654 | WP_015387348 | hypothetical protein | - |
| HXH17_RS00215 | 30019..31143 | - | 1125 | WP_000486719 | site-specific integrase | - |
| HXH17_RS00220 | 31192..31500 | + | 309 | WP_001199278 | hypothetical protein | - |
| HXH17_RS00450 | 31783..32004 | + | 222 | Protein_44 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| HXH17_RS00230 | 32001..33377 | - | 1377 | WP_000750519 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| HXH17_RS00235 | 33390..34025 | - | 636 | WP_000934978 | A24 family peptidase | - |
| HXH17_RS00240 | 34029..34457 | - | 429 | WP_001326801 | lytic transglycosylase domain-containing protein | virB1 |
| HXH17_RS00245 | 34578..35135 | - | 558 | WP_000095051 | type 4 pilus major pilin | - |
| HXH17_RS00250 | 35180..36289 | - | 1110 | WP_000974900 | type II secretion system F family protein | - |
| HXH17_RS00255 | 36280..37818 | - | 1539 | WP_001420475 | ATPase, T2SS/T4P/T4SS family | virB11 |
| HXH17_RS00260 | 37843..38337 | - | 495 | WP_000912551 | type IV pilus biogenesis protein PilP | - |
| HXH17_RS00265 | 38321..39643 | - | 1323 | WP_000454143 | type 4b pilus protein PilO2 | - |
| HXH17_RS00270 | 39682..41325 | - | 1644 | WP_001035590 | PilN family type IVB pilus formation outer membrane protein | - |
| HXH17_RS00275 | 41318..41836 | - | 519 | WP_015387353 | YfdA protein | virb4 |
| HXH17_RS00280 | 41883..43841 | - | 1959 | WP_000338974 | type IV secretory system conjugative DNA transfer family protein | - |
| HXH17_RS00285 | 43857..44912 | - | 1056 | WP_001059977 | P-type DNA transfer ATPase VirB11 | virB11 |
| HXH17_RS00290 | 44931..46070 | - | 1140 | WP_015387354 | TrbI/VirB10 family protein | virB10 |
| HXH17_RS00295 | 46060..46761 | - | 702 | WP_053882504 | TrbG/VirB9 family P-type conjugative transfer protein | - |
| HXH17_RS00300 | 46827..47561 | - | 735 | WP_000432282 | type IV secretion system protein | virB8 |
| HXH17_RS00305 | 47725..50082 | - | 2358 | WP_015387356 | VirB4 family type IV secretion system protein | virb4 |
| HXH17_RS00310 | 50088..50408 | - | 321 | WP_000362081 | VirB3 family type IV secretion system protein | virB3 |
| HXH17_RS00445 | 50479..50769 | - | 291 | WP_000865479 | conjugal transfer protein | - |
| HXH17_RS00320 | 50769..51353 | - | 585 | WP_001401693 | lytic transglycosylase domain-containing protein | virB1 |
| HXH17_RS00325 | 51374..51772 | - | 399 | WP_001708012 | hypothetical protein | - |
| HXH17_RS00330 | 52112..52549 | - | 438 | WP_015387358 | type IV pilus biogenesis protein PilM | - |
| HXH17_RS00335 | 52555..53790 | - | 1236 | WP_001419735 | TcpQ domain-containing protein | - |
| HXH17_RS00340 | 53793..54092 | - | 300 | WP_000835763 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| HXH17_RS00345 | 54159..54791 | - | 633 | WP_001419736 | hypothetical protein | - |
| HXH17_RS00350 | 54839..55648 | + | 810 | WP_001419737 | DUF5710 domain-containing protein | - |
| HXH17_RS00355 | 55695..55931 | - | 237 | WP_000750964 | EexN family lipoprotein | - |
| HXH17_RS00360 | 55935..56582 | - | 648 | WP_001419738 | type IV secretion system protein | - |
| HXH17_RS00365 | 56588..57574 | - | 987 | WP_001419739 | type IV secretion system protein | virB6 |
| HXH17_RS00370 | 57578..57835 | - | 258 | WP_000739144 | hypothetical protein | - |
| HXH17_RS00375 | 57832..58134 | - | 303 | WP_001360345 | hypothetical protein | - |
| HXH17_RS00380 | 58150..58401 | - | 252 | WP_015387362 | hypothetical protein | - |
| HXH17_RS00385 | 58398..58580 | - | 183 | WP_022540746 | hypothetical protein | - |
| HXH17_RS00390 | 58549..59211 | - | 663 | WP_001419740 | hypothetical protein | - |
| HXH17_RS00395 | 59222..59392 | - | 171 | WP_000550721 | hypothetical protein | - |
| HXH17_RS00400 | 59396..59839 | - | 444 | WP_000964840 | NfeD family protein | - |
| HXH17_RS00405 | 59912..60865 | - | 954 | WP_072105959 | SPFH domain-containing protein | - |
| HXH17_RS00410 | 60911..61105 | - | 195 | WP_000049865 | DUF1187 family protein | - |
| HXH17_RS00415 | 61098..61604 | - | 507 | WP_001358485 | CaiF/GrlA family transcriptional regulator | - |
Host bacterium
| ID | 7944 | GenBank | NZ_LC477293 |
| Plasmid name | pUIH-1 | Incompatibility group | IncI2 |
| Plasmid size | 62194 bp | Coordinate of oriT [Strand] | 18155..18207 [-] |
| Host baterium | Shigella sonnei strain UIH-1 |
Cargo genes
| Drug resistance gene | blaCTX-M-64 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |