Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 107502
• Name: oriT_pS-2S
• Organism: Salmonella enterica subsp. enterica serovar Albany strain A17-KCI-MNK-002-2S
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_MW052847 (63042..63130 [-], 89 nt)
• oriT length: 89 nt
• IRs (inverted repeats): _
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 89 nt

>oriT_pS-2S
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGTAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTACACATCCTGTCCCGTTTTTC

oriT secondary structure

Predicted by RNAfold.

Relaxase

nikB_MTW35_RS00405_pS-2S

• ID: 5025
• GenBank: WP_001474686
• Name: nikB_MTW35_RS00405_pS-2S
• UniProt ID: A0A2J0RB70
• Length: 899 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 899 a.a.
• Molecular weight: 103834.33 Da
• Isoelectric Point: 7.3473

>WP_001474686.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELNLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIAQQAHYAKDNTDPVFHYILSWQAHESPRPEQIYDSVR
HTLKSLGLGEHQYVSAVHTDTDNLHVHVAVNRVHPVTGYLNCLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFLQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDKSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (62-314)

Protein structure

• AlphaFold DB: A0A2J0RB70

Auxiliary protein

WP_001283947_pS-2S

• ID: 2304
• GenBank: WP_001283947
• Name: WP_001283947_pS-2S
• UniProt ID: A0A142CMC2
• Length: 110 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

Auxiliary protein sequence

• Length: 110 a.a.
• Molecular weight: 12613.57 Da
• Isoelectric Point: 10.7463

>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

Protein domains

No domain identified.

Protein structure

• AlphaFold DB: A0A142CMC2

T4CP

trbC_MTW35_RS00410_pS-2S

• ID: 5234
• GenBank: WP_001289271
• Name: trbC_MTW35_RS00410_pS-2S
• UniProt ID: _
• Length: 763 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 763 a.a.
• Molecular weight: 86927.12 Da
• Isoelectric Point: 6.7713

>WP_001289271.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKIDVQELIELH
PGEFFSIFRGETVPSASFFIPDNEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTAQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHLTGRPVNGFHHKKTNRPDWDGMY

Protein domains

No domain identified.

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 68508..96567

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MTW35_RS00410	66224..68515	-	2292	WP_001289271	F-type conjugative transfer protein TrbC	-
MTW35_RS00415	68508..69578	-	1071	WP_000151575	IncI1-type conjugal transfer protein TrbB	trbB
MTW35_RS00420	69597..70805	-	1209	WP_000121273	IncI1-type conjugal transfer protein TrbA	trbA
MTW35_RS00425	71097..71249	+	153	WP_001303307	Hok/Gef family protein	-
MTW35_RS00430	71321..71572	-	252	WP_001291965	hypothetical protein	-
MTW35_RS00435	71615..71779	+	165	WP_001323923	hypothetical protein	-
MTW35_RS00440	71933..72715	-	783	WP_001300609	IS21-like element IS100kyp family helper ATPase IstB	-
MTW35_RS00445	72712..73734	-	1023	WP_001572805	IS21-like element IS100 family transposase	-
MTW35_RS00450	74455..74739	-	285	WP_014640317	hypothetical protein	-
MTW35_RS00455	75023..75232	+	210	WP_000062603	HEAT repeat domain-containing protein	-
MTW35_RS00460	75304..75966	-	663	WP_000644794	plasmid IncI1-type surface exclusion protein ExcA	-
MTW35_RS00465	76037..78205	-	2169	WP_000698368	DotA/TraY family protein	traY
MTW35_RS00470	78302..78886	-	585	WP_001037998	conjugal transfer protein TraX	-
MTW35_RS00475	78915..80117	-	1203	WP_001189158	IncI1-type conjugal transfer protein TraW	traW
MTW35_RS00480	80084..80698	-	615	WP_000337394	IncI1-type conjugal transfer protein TraV	traV
MTW35_RS00485	80698..83742	-	3045	WP_001024751	IncI1-type conjugal transfer protein TraU	traU
MTW35_RS00490	83832..84632	-	801	WP_001164788	IncI1-type conjugal transfer protein TraT	traT
MTW35_RS00495	84616..84804	-	189	WP_001277255	putative conjugal transfer protein TraS	-
MTW35_RS00500	84868..85272	-	405	WP_000086960	IncI1-type conjugal transfer protein TraR	traR
MTW35_RS00505	85323..85850	-	528	WP_001055569	conjugal transfer protein TraQ	traQ
MTW35_RS00510	85850..86554	-	705	WP_000801920	IncI1-type conjugal transfer protein TraP	traP
MTW35_RS00515	86554..87843	-	1290	WP_001271997	conjugal transfer protein TraO	traO
MTW35_RS00520	87846..88829	-	984	WP_001191878	IncI1-type conjugal transfer protein TraN	traN
MTW35_RS00525	88840..89532	-	693	WP_000138552	DotI/IcmL family type IV secretion protein	traM
MTW35_RS00530	89529..89876	-	348	WP_001055900	conjugal transfer protein	traL
MTW35_RS00535	89894..93661	-	3768	WP_001141535	LPD7 domain-containing protein	-
MTW35_RS00540	93751..94302	-	552	WP_000014584	phospholipase D family protein	-
MTW35_RS00545	94317..94607	-	291	WP_001299214	hypothetical protein	traK
MTW35_RS00550	94604..95752	-	1149	WP_001024972	plasmid transfer ATPase TraJ	virB11
MTW35_RS00555	95749..96567	-	819	WP_000646097	IncI1-type conjugal transfer lipoprotein TraI	traI
MTW35_RS00560	96564..97022	-	459	WP_001079808	IncI1-type conjugal transfer lipoprotein TraH	-
MTW35_RS00565	97417..98001	-	585	WP_000977517	histidine phosphatase family protein	-

Host bacterium

• ID: 7937
• GenBank: NZ_MW052847
• Plasmid name: pS-2S
• Incompatibility group: IncI1
• Plasmid size: 98987 bp
• Coordinate of oriT [Strand]: 63042..63130 [-]
• Host baterium: Salmonella enterica subsp. enterica serovar Albany strain A17-KCI-MNK-002-2S

Cargo genes

• Drug resistance gene: blaCMY-2
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -