Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   107501
Name   oriT_pSRC119-A/C in_silico
Organism   Salmonella enterica subsp. enterica serovar Senftenberg strain SRC119
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_KM670336 (64948..65098 [+], 151 nt)
oriT length   151 nt
IRs (inverted repeats)      93..98, 106..111  (TGGCCT..AGGCCA)
 12..17, 24..29  (AACCCT..AGGGTT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 151 nt

>oriT_pSRC119-A/C
TGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAA

Visualization of oriT structure



Relaxase

ID   5024 GenBank   WP_001337757
Name   mobH_HTF15_RS00455_pSRC119-A/C insolico UniProt ID   A0A709FRI2
Length   992 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 992 a.a.        Molecular weight: 109581.85 Da        Isoelectric Point: 4.8956

>WP_001337757.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIETAPSARVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEADDGDDQEEGEAALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGPTESSK
PDAGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPF
DAFNASAETTSTDATNSEIPDVAMPGKQEEQPKQDFVPQEQNSLQGDDFPMFGGSDEPPSWAIEPLPMLT
DAPEQPTHTPEMPHTDNVNQHEKDAKTLLVEMLSGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVI
LYPDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQ
DAFELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKASPEQKAKGKDSQPQPKEKKVDVTSPVEEQQ
RKPVQEKQNVARLPKREAQPVAPEPKVEREKELGHVEVREREDPEVREFEPPKAKTNPKDINAEDFLPSG
VTPQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKK
RQGKLYLEVNET

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure


Source ID Structure
AlphaFold DB A0A709FRI2


T4CP

ID   5232 GenBank   WP_000178856
Name   traD_HTF15_RS00460_pSRC119-A/C insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 69197.66 Da        Isoelectric Point: 6.9403

>WP_000178856.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTMSYDPLAYEMPWRPNYEKNAVAGWLAASGAALAVEQVSTMPPEPFYWMTGICGVMAMARLPKAIKLHL
LQKHLKGRDLEFISIAELQKYIKDTPDDMWLGSGFLWENRHAQRVFEILKRDWTSIVGRESTVKKVVRKI
QGKKKELPIGQPWIHGVEPKEEKLMQPLKHTEGHSLIVGTTGSGKTRMFDILISQAILRGEAVIIIDPKG
DKEMRDNARRACEAMGQPERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEAGADPFKSFGWQ
ALNNIAQGLVITHDRPNLTKLRRFLEGGAAGLVIKAVQAYSERVMPDWEAEAAAYLEKAKNGSREKIAFA
LMKFYYDIIQPEHPNSDLEGLLSMFQHDQTHFSKMVANLLPIMNMLTSGELGPLLSPDSSDLSDERQITD
SAKIINNAQVAYLGLDSLTDNMVGSAMGSIFLSDLTAVAGDRYNYGVNNRPVNIFVDEAAEVINDPFIQL
LNKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNTFALRIVDGETQEYIADNLPKTRLKYVMRT
QGQNSDGKEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGTIVKGRLPILTQ

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(471-598)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(170-297)

  Protein structure



No available structure.



ID   5233 GenBank   WP_000637386
Name   traC_HTF15_RS00540_pSRC119-A/C insolico UniProt ID   _
Length   815 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 815 a.a.        Molecular weight: 92590.87 Da        Isoelectric Point: 5.9222

>WP_000637386.1 MULTISPECIES: type IV secretion system protein TraC [Gammaproteobacteria]
MIVTIKKKLEETLIPEHLRAAGIIPVLAYDEDDHVFLMDDHSAGFGFMCEPLCGADEKVQERMNGFLNQE
FPSKTTLQFVLFRSPDINQEMYRMMGLRDGFRHELLTSVIKERINFLQHHTTERIFAKTNKGIYDNGLIQ
DLKLFVTCKVPIKNNNPTESELQQLAQLRTKVESSLQTVGLRPRTMTAVNYIRIMSTILNWGPDASWRHD
SVDWEMDKPICEQIFDYGTDVEVSKNGIRLGDYHAKVMSAKKLPDVFYFGDALTYAGDLSGGNSSIKENY
MVVTNVFFPEAESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTLYESMKEGAKPVKITYSVVLF
APTKERVEAAAMAARNIWRESRFELMEDKFVALPMFLNCLPFCTDRDAVRDLFRYKTMTTEQAAVVLPVF
GEWKGTGTYHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELIFSYLSEGAQVWVIDAGKS
YQKLSEMLNGDFVHFEEGTHVCLNPFELIQNYEDEEDAIVSLVCAMASAKGLLDEWQISALKQVLSRLWE
EKGKEMKVDDIAERCLEEENDQRLKDIGQQLYAFTSKGSYGKYFSRKNNVSFQNQFTVLELDELQGRKHL
RQVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSVFMEHAYRKFRKYGGSVVIATQSINDLY
ENAVGRAIAENSASMYLLGQTEETVESVKRSGRLTLSEGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRL
IVGDFQKLLYSTDPVDVNAIDQFVKQGMSIPEAIKAVMRSRQQAA

  Protein domains


Predicted by InterproScan.

TraG P-loop domain (P-loop_TraG)

(449-809)

Family of unknown function (DUF5934) (DUF5934)

(284-434)

TraC protein (TraC_F_IV)

(21-255)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 63032..88223

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTF15_RS00445 59057..59290 - 234 WP_001191892 hypothetical protein -
HTF15_RS00450 59272..59889 - 618 WP_001249396 hypothetical protein -
HTF15_RS00455 60057..63035 + 2979 WP_001337757 MobH family relaxase -
HTF15_RS00460 63032..64897 + 1866 WP_000178856 conjugative transfer system coupling protein TraD virb4
HTF15_RS00465 64908..65492 + 585 WP_000332866 hypothetical protein -
HTF15_RS00470 65449..66078 + 630 WP_000743450 DUF4400 domain-containing protein tfc7
HTF15_RS00475 66088..66534 + 447 WP_000122506 hypothetical protein -
HTF15_RS00480 66544..66921 + 378 WP_000869261 hypothetical protein -
HTF15_RS00485 66921..67583 + 663 WP_001231463 hypothetical protein -
HTF15_RS00490 67764..67907 + 144 WP_001275801 hypothetical protein -
HTF15_RS00495 67919..68284 + 366 WP_001052531 hypothetical protein -
HTF15_RS00500 68429..68710 + 282 WP_000805625 type IV conjugative transfer system protein TraL traL
HTF15_RS00505 68707..69333 + 627 WP_001049716 TraE/TraK family type IV conjugative transfer system protein traE
HTF15_RS00510 69317..70234 + 918 WP_000794249 type-F conjugative transfer system secretin TraK traK
HTF15_RS00515 70234..71547 + 1314 WP_024131605 TraB/VirB10 family protein traB
HTF15_RS00520 71544..72122 + 579 WP_000793435 type IV conjugative transfer system lipoprotein TraV traV
HTF15_RS00525 72126..72518 + 393 WP_000479535 TraA family conjugative transfer protein -
HTF15_RS00530 72719..78250 + 5532 WP_095762596 Ig-like domain-containing protein -
HTF15_RS00535 78399..79106 + 708 WP_001259347 DsbC family protein -
HTF15_RS00540 79103..81550 + 2448 WP_000637386 type IV secretion system protein TraC virb4
HTF15_RS00545 81565..81882 + 318 WP_000351984 hypothetical protein -
HTF15_RS00550 81879..82409 + 531 WP_001010738 S26 family signal peptidase -
HTF15_RS00555 82372..83637 + 1266 WP_000621288 TrbC family F-type conjugative pilus assembly protein traW
HTF15_RS00560 83634..84305 + 672 WP_001337754 EAL domain-containing protein -
HTF15_RS00565 84305..85309 + 1005 WP_043940352 TraU family protein traU
HTF15_RS00570 85425..88223 + 2799 WP_001256487 conjugal transfer mating pair stabilization protein TraN traN
HTF15_RS00575 88262..89122 - 861 WP_000709517 hypothetical protein -
HTF15_RS00580 89245..89886 - 642 WP_000796664 hypothetical protein -
HTF15_RS00585 90180..90500 + 321 WP_000547566 hypothetical protein -
HTF15_RS00590 90804..90989 + 186 WP_001186917 hypothetical protein -
HTF15_RS00595 91209..92177 + 969 WP_000085162 AAA family ATPase -
HTF15_RS00600 92188..93096 + 909 WP_000739139 hypothetical protein -

Region 2: 144631..170894

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTF15_RS00895 140249..141274 + 1026 WP_000742814 aminoglycoside O-phosphotransferase APH(4)-Ia -
HTF15_RS00900 141349..141633 + 285 Protein_179 AAC(3) family N-acetyltransferase -
HTF15_RS00905 141696..142448 - 753 WP_000602738 IS6 family transposase -
HTF15_RS00910 142510..144033 + 1524 Protein_181 Tn3 family transposase -
HTF15_RS00915 144099..144569 - 471 WP_000168937 KfrB domain-containing protein -
HTF15_RS00920 144631..146091 - 1461 WP_050451052 TrbI/VirB10 family protein virB10
HTF15_RS00925 146088..146546 - 459 WP_033474588 conjugal transfer protein TrbH -
HTF15_RS00930 146548..147333 - 786 WP_238785731 P-type conjugative transfer protein TrbG virB9
HTF15_RS00935 147486..148184 - 699 WP_004167166 type IV secretion system protein virB8
HTF15_RS00940 148274..148579 - 306 WP_004167161 hypothetical protein -
HTF15_RS00945 148583..150301 - 1719 WP_033474589 P-type conjugative transfer protein TrbL virB6
HTF15_RS00950 150305..150508 - 204 WP_004167247 hypothetical protein -
HTF15_RS00955 150509..151297 - 789 WP_004167246 P-type conjugative transfer protein TrbJ virB5
HTF15_RS00960 151266..153773 - 2508 WP_004167244 transporter virb4
HTF15_RS00965 153787..154059 - 273 WP_281040200 conjugal transfer protein TrbD virB3
HTF15_RS00970 154118..154822 + 705 WP_001067855 IS6-like element IS26 family transposase -
HTF15_RS00975 155097..156110 - 1014 WP_000845054 class 1 integron integrase IntI1 -
HTF15_RS00980 156195..156329 + 135 WP_227498430 DUF1010 domain-containing protein -
HTF15_RS00985 156335..157173 + 839 Protein_196 AadA family aminoglycoside 3''-O-nucleotidyltransferase -
HTF15_RS00990 157290..157637 + 348 WP_000679427 quaternary ammonium compound efflux SMR transporter QacE delta 1 -
HTF15_RS00995 157631..158470 + 840 WP_000259031 sulfonamide-resistant dihydropteroate synthase Sul1 -
HTF15_RS01000 158598..159098 + 501 WP_000376623 GNAT family N-acetyltransferase -
HTF15_RS01005 159067..160059 - 993 WP_001381192 TniB family NTP-binding protein -
HTF15_RS01010 160062..161741 - 1680 WP_172686663 Mu transposase C-terminal domain-containing protein -
HTF15_RS01075 161816..161896 - 81 Protein_202 EAL domain-containing protein -
HTF15_RS01015 161882..162817 + 936 Protein_203 UvrD-helicase domain-containing protein -
HTF15_RS01020 162928..163968 + 1041 WP_001326396 conjugal transfer protein TraF traF
HTF15_RS01025 163970..165403 + 1434 WP_000256129 conjugal transfer protein TraH traH
HTF15_RS01030 165416..169030 + 3615 WP_000534551 conjugal transfer protein TraG N-terminal domain-containing protein traG
HTF15_RS01035 169068..169430 - 363 WP_000683483 hypothetical protein -
HTF15_RS01040 170146..170418 + 273 WP_000356489 helix-turn-helix domain-containing protein -
HTF15_RS01045 170418..170894 + 477 WP_094868284 transglycosylase SLT domain-containing protein virB1
HTF15_RS01050 170962..171570 + 609 WP_000891157 hypothetical protein -
HTF15_RS01055 171567..172118 + 552 WP_001020646 FlhC family transcriptional regulator -
HTF15_RS01060 172178..172597 - 420 WP_000651490 H-NS family nucleoid-associated regulatory protein -
HTF15_RS01065 172599..172892 - 294 WP_000919078 hypothetical protein -
HTF15_RS01070 172909..173892 - 984 WP_000077458 ParM/StbA family protein -

Host bacterium

ID   7936 GenBank   NZ_KM670336
Plasmid name   pSRC119-A/C Incompatibility group   IncA/C2
Plasmid size   174068 bp Coordinate of oriT [Strand]   64948..65098 [+]
Host baterium   Salmonella enterica subsp. enterica serovar Senftenberg strain SRC119

Cargo genes

Drug resistance gene   aph(3')-Ia, tet(D), erm(42), sul2, aac(3)-IVa, aph(4)-Ia, aadA2, qacE, sul1
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -