Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 107497
• Name: oriT_pSAN1-1175
• Organism: Salmonella enterica subsp. enterica serovar Anatum str. USDA-ARS-USMARC-1175
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP019898 (9079..9123 [+], 45 nt)
• oriT length: 45 nt
• IRs (inverted repeats): 7..13, 25..31 (CGTTTCT..AGAAACG)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 45 nt

>oriT_pSAN1-1175
GCGCACCGTTTCTGAACGAAGTGAAGAAACGTCTAAGTGCGCCCT

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobA_AW55_RS00065_pSAN1-1175

• ID: 5020
• GenBank: WP_016946648
• Name: mobA_AW55_RS00065_pSAN1-1175
• UniProt ID: A0A500IE68
• Length: 371 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 371 a.a.
• Molecular weight: 43186.77 Da
• Isoelectric Point: 6.4038

>WP_016946648.1 MULTISPECIES: mobilization protein MobA [Gammaproteobacteria]
MASYHLSVKTGGKGSASPHADYIAREGKYAREKDSDLEHKESGNMPAWAAHKPSEFWKAADTSERANGCT
YREIEIALPRELKPEQRLELVRDFVQQEIGDRHAYQFAIHNPKAAIAGGEQPHAHIMFSERINDGIHRDP
EQYFKRANTKEPERGGAKKARFGETPTERKEYLVAQRERWADLQNKHLERYQHADRVDSRSLKAQGIDRE
PERHLGAGQVQRFDTEQLQAILERREAERQVQQSRDERDSVIDVTTSLREALSERDTLTLKQELKSEPEQ
ESHSGRTFDFEKEPDKLNALVSDAMKDIQEEIDLQSLVNDAMAEFQGIHQEMERQRERERLAEKQRQQEK
ERQRLAEQIRQKPDKGWSFSR

Protein domains

Predicted by InterproScan.

• MobA/MobL family (MobA_MobL) (42-218)

Protein structure

• AlphaFold DB: A0A500IE68

Host bacterium

• ID: 7932
• GenBank: NZ_CP019898
• Plasmid name: pSAN1-1175
• Incompatibility group: ColE10
• Plasmid size: 10280 bp
• Coordinate of oriT [Strand]: 9079..9123 [+]
• Host baterium: Salmonella enterica subsp. enterica serovar Anatum str. USDA-ARS-USMARC-1175

Cargo genes

• Drug resistance gene: tet(C)
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -