Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   107494
Name   oriT_p16-174478-1 in_silico
Organism   Salmonella enterica subsp. enterica serovar Napoli
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MN651559 (37280..37368 [-], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_p16-174478-1
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGTAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTACACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   5017 GenBank   WP_001405892
Name   nikB_J1814_RS00245_p16-174478-1 insolico UniProt ID   A0A3V4WX06
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 103883.32 Da        Isoelectric Point: 7.3373

>WP_001405892.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIAQQAHYAKDNTDPVFHYILSWQAHESPRPEQIYDSVR
HTLKSLGLGEHQYVSAVHTDTDNLHVHVAVNRVHPVTGYLNCLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB A0A3V4WX06


Auxiliary protein

ID   2298 GenBank   WP_001283947
Name   WP_001283947_p16-174478-1 insolico UniProt ID   A0A142CMC2
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12613.57 Da        Isoelectric Point: 10.7463

>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A142CMC2


T4CP

ID   5221 GenBank   WP_001289276
Name   trbC_J1814_RS00250_p16-174478-1 insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86941.11 Da        Isoelectric Point: 6.7713

>WP_001289276.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 42746..80200

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J1814_RS00250 (p16_056) 40462..42753 - 2292 WP_001289276 F-type conjugative transfer protein TrbC -
J1814_RS00255 (p16_057) 42746..43816 - 1071 WP_000151583 IncI1-type conjugal transfer protein TrbB trbB
J1814_RS00260 (p16_058) 43835..45043 - 1209 WP_000121273 IncI1-type conjugal transfer protein TrbA trbA
J1814_RS00265 (p16_060) 45319..45471 + 153 WP_001331364 Hok/Gef family protein -
J1814_RS00270 (p16_061) 45543..45794 - 252 WP_001291964 hypothetical protein -
J1814_RS00520 46293..46388 + 96 WP_001303310 DinQ-like type I toxin DqlB -
J1814_RS00275 46453..46737 - 285 WP_206606954 hypothetical protein -
J1814_RS00280 (p16_063) 47021..47230 + 210 WP_000062603 HEAT repeat domain-containing protein -
J1814_RS00285 (p16_064) 47302..47976 - 675 WP_348541436 plasmid IncI1-type surface exclusion protein ExcA -
J1814_RS00290 (p16_066) 48026..50194 - 2169 WP_000698358 DotA/TraY family protein traY
J1814_RS00295 (p16_067) 50291..50875 - 585 WP_001037982 IncI1-type conjugal transfer protein TraX -
J1814_RS00300 (p16_068) 50904..52106 - 1203 WP_001189160 IncI1-type conjugal transfer protein TraW traW
J1814_RS00305 (p16_069) 52073..52687 - 615 WP_000337399 IncI1-type conjugal transfer protein TraV traV
J1814_RS00310 (p16_070) 52687..55731 - 3045 WP_001024782 IncI1-type conjugal transfer protein TraU traU
J1814_RS00315 (p16_071) 55821..56621 - 801 WP_001164788 IncI1-type conjugal transfer protein TraT traT
J1814_RS00320 (p16_072) 56605..56793 - 189 WP_001277255 putative conjugal transfer protein TraS -
J1814_RS00325 (p16_073) 56857..57261 - 405 WP_000086959 IncI1-type conjugal transfer protein TraR traR
J1814_RS00330 (p16_074) 57312..57839 - 528 WP_001055569 conjugal transfer protein TraQ traQ
J1814_RS00335 (p16_075) 57839..58543 - 705 WP_000801919 IncI1-type conjugal transfer protein TraP traP
J1814_RS00340 (p16_076) 58543..59832 - 1290 WP_001272000 conjugal transfer protein TraO traO
J1814_RS00345 (p16_077) 59835..60818 - 984 WP_001191879 IncI1-type conjugal transfer protein TraN traN
J1814_RS00350 (p16_078) 60829..61521 - 693 WP_000138550 DotI/IcmL family type IV secretion protein traM
J1814_RS00355 (p16_079) 61518..61865 - 348 WP_001055900 conjugal transfer protein traL
J1814_RS00360 (p16_080) 61883..65650 - 3768 WP_001141526 LPD7 domain-containing protein -
J1814_RS00365 (p16_082) 65740..66291 - 552 WP_000014583 phospholipase D family protein -
J1814_RS00370 (p16_083) 66306..66596 - 291 WP_001299214 hypothetical protein traK
J1814_RS00375 (p16_084) 66593..67741 - 1149 WP_001024972 plasmid transfer ATPase TraJ virB11
J1814_RS00380 (p16_085) 67738..68556 - 819 WP_000646098 IncI1-type conjugal transfer lipoprotein TraI traI
J1814_RS00385 (p16_086) 68553..69011 - 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
J1814_RS00390 (p16_087) 69406..69990 - 585 WP_000977522 histidine phosphatase family protein -
J1814_RS00395 (p16_088) 70050..71252 - 1203 WP_000976351 conjugal transfer protein TraF -
J1814_RS00400 (p16_089) 71337..72161 - 825 WP_001238939 conjugal transfer protein TraE traE
J1814_RS00405 (p16_090) 72312..73466 - 1155 WP_001139957 site-specific integrase -
J1814_RS00525 74052..74273 + 222 Protein_82 prepilin -
J1814_RS00415 (p16_091) 74270..75694 - 1425 WP_001389385 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
J1814_RS00420 (p16_092) 75694..76350 - 657 WP_160342610 prepilin peptidase -
J1814_RS00425 (p16_093) 76335..76895 - 561 WP_160342611 lytic transglycosylase domain-containing protein virB1
J1814_RS00430 (p16_094) 76905..77519 - 615 WP_000959786 type 4 pilus major pilin -
J1814_RS00435 (p16_095) 77537..78634 - 1098 WP_001208805 type II secretion system F family protein -
J1814_RS00440 (p16_096) 78647..80200 - 1554 WP_000362202 ATPase, T2SS/T4P/T4SS family virB11
J1814_RS00445 (p16_097) 80211..80663 - 453 WP_001247334 type IV pilus biogenesis protein PilP -
J1814_RS00450 (p16_098) 80650..81945 - 1296 WP_021560540 type 4b pilus protein PilO2 -
J1814_RS00455 (p16_099) 81938..83620 - 1683 WP_000748143 PilN family type IVB pilus formation outer membrane protein -
J1814_RS00460 (p16_100) 83634..84071 - 438 WP_000539807 type IV pilus biogenesis protein PilM -
J1814_RS00465 (p16_101) 84071..85138 - 1068 WP_001302629 type IV pilus biogenesis lipoprotein PilL -

Host bacterium

ID   7929 GenBank   NZ_MN651559
Plasmid name   p16-174478-1 Incompatibility group   IncI1
Plasmid size   90272 bp Coordinate of oriT [Strand]   37280..37368 [-]
Host baterium   Salmonella enterica subsp. enterica serovar Napoli

Cargo genes

Drug resistance gene   blaCTX-M-15
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -