Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 107485
• Name: oriT_pBU108a
• Organism: Staphylococcus aureus strain A960649
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_KF831355 (25149..25337 [-], 189 nt)
• oriT length: 189 nt
• IRs (inverted repeats): 149..155, 162..168 (CTATCAT..ATGATAG) 132..138, 142..148 (GTCTGGC..GCCAGAC) 33..38, 45..50 (TGTCAC..GTGACA)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 189 nt

>oriT_pBU108a
TGTCACAAAAATAAGACAAAATCTACATGTAGTGTCACAAAACCGTGACATACACAATATATAGTGTCACAAAAATGTGACACTAGGTGTTTTTATGATATCACTATGAAATGTCCTAAAACCCTTGGAATGTCTGGCTTTGCCAGACCTATCATTGTCCGATGATAGCAAATTCCCCTTATGCTCTTA

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobP2_HTB97_RS00160_pBU108a

• ID: 5007
• GenBank: WP_224743029
• Name: mobP2_HTB97_RS00160_pBU108a
• UniProt ID: _
• Length: 383 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 383 a.a.
• Molecular weight: 45810.10 Da
• Isoelectric Point: 9.9143

>WP_224743029.1 MobP2 family relaxase [Staphylococcus aureus]
MMPKIVTVSQFEQPNEFYEYSNYVNYMKRGEAQNNKDDFKYDVYAHYMFDEEKSQNMFNEHDNFMSKKAI
NKIKSDFSHAQKNKGMMWKDVISFDNSALESVGIYDSKTHTLDEQKIKEATRKMMKRFEKKEGLEENLVW
SGAIHYNTDNIHVHVAAVEKVIKRTRGKRKPATLKHMKSTFANELFDIKGERQKINDFIRERIVKGIRDE
SEPCKDKAMKKQIKKVHKLLEDIPRNQWNYNNNILKYIRPEIDKITDIYIKNYHSKDFEVFKNDLKRQTN
LYKETYGENSNYGSYEKTKMKDLYARSGNTILKNIKELDQDLMKVKQQSTHSKSNATITKLKIGKCLNQA
LYRTKYALRSDYDKEKNIQQYYQEFDKQSYRER

Protein domains

Predicted by InterproScan.

• MobL relaxases (MobL) (2-358)

Protein structure

No available structure.

T4CP

t4cp2_HTB97_RS00005_pBU108a

• ID: 5210
• GenBank: WP_031919407
• Name: t4cp2_HTB97_RS00005_pBU108a
• UniProt ID: _
• Length: 923 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 923 a.a.
• Molecular weight: 106214.32 Da
• Isoelectric Point: 8.0720

>WP_031919407.1 VirD4-like conjugal transfer protein, CD1115 family [Staphylococcus aureus]
MRFGSKDKHGYRRKKLSFSQNKHLLAHPCILVSILLLIMLLATAIANFLINILMTVFFGNHDIFTNMKQL
NTKYTDYIFNFRLDAVLLYIPIWVLVFIFFGKKVYQFRQRFKSLNNNEKASGRFATRKEITQQYKAIPQR
EHAFEGEGGLPVAMFGVYDFVTKTKQYLEQRKTAIREINGITDLTADEKLELRKDKLGKMNKEFATTFFS
KREFTFIDESPTNNLIVGTSRSGKGEIVVLSMIENYSRSSEQPSLIVNDMKGELFSASYKTLENRGYQVE
IFNLDQPMESTMSFNPLQQVIDEYMKGDLGEAQEMTKQITYTLTNNEGVEDNEWNLMAGALINAMILALC
EETLPKNPEKVTLYSVSNMLQTLSTKRFYKEIAIGNKVQLIEVSALDEYMERFPSHSPAKTQYATVQAAP
DKMRSSIIGTALKALQPFTTDTIAKLTSHTSFDINKLGFPSIIDGYATKDSTFELGVQVLRDGENGIEYE
EVEGIQIGVNEYGYWQYPFKTVLKVGDRIETIEKDGNNQVITSYFYVDHIDDKGQVTFNQKGELDNSDIT
IRKFNSFPKPIAVFMVVPDYNNANHGIASILVNQIVFEISKNSQLYTENQSTYRRVIFHLDEAGNMPPIP
NLSQKVNVSLSRGLRFNFFVQAFSQFKDKYGDAYDAIMDACQNKVYIMATQEQTLKDFSAMIGSQQITVH
SRSGEAGAIKSSITENQEERPLLRPDELARLQEGETVVVRNLKRQDKKRNKVMSYPIFNDGKYAMKYRYQ
YLSDLMDTSQSIIHFRPMLKARCEHRHLQLEATLVDWDKRIEEMQEGIDGQKEENKNINDINQYKKSNEE
GNVAVRVKRLETLKEKVGDAIFDKITRRFERYLRTYAEQGKNVNTITQKKLEELVLADSEVKEEEKTKRI
EMIKKLIKEAKTT

Protein domains

Predicted by InterproScan.

• TraM recognition site of TraD and TraG (TraG-D_C) (615-732)

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (209-458)

Protein structure

No available structure.

Host bacterium

• ID: 7920
• GenBank: NZ_KF831355
• Plasmid name: pBU108a
• Incompatibility group: -
• Plasmid size: 34936 bp
• Coordinate of oriT [Strand]: 25149..25337 [-]
• Host baterium: Staphylococcus aureus strain A960649

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA21