Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   107484
Name   oriT_pLRSA417 in_silico
Organism   Staphylococcus aureus strain 417
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_KJ922127 (36490..36525 [+], 36 nt)
oriT length   36 nt
IRs (inverted repeats)     _
Location of nic site      29..30
Conserved sequence flanking the
  nic site    		  GTGCGCCCTT
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 36 nt

>oriT_pLRSA417
CACGCGATCGGAACGTTCGCATAAGTGCGCCCTTAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   5005 GenBank   WP_030061449
Name   MobA_MobL_HTC99_RS00180_pLRSA417 insolico UniProt ID   A0A848EWD6
Length   665 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 665 a.a.        Molecular weight: 78725.60 Da        Isoelectric Point: 5.1613

>WP_030061449.1 MULTISPECIES: MobA/MobL family protein [Staphylococcus]
MAMYHFQNKFVSKANGQSATAKAAYNSASRIKDFKENEFKDYSNKQCDYSEILLPNNADDKFKDREYLWN
KVNDVENRKNSQVAREIIIGLPNEFDPNSNIELAKEFAESLSNEGMIVDLNIHKINDENPHAHLLCTLRG
LDKNNEFEPKRKGNDYIRDWNTKDKHNEWRKRWETVQNKHLEMNGFSVRVSADSYKKQNIDLEPTKKEGW
KARKFEDETGKKSSISKHNESIKKRNQQKINKMFDEVDDMKSHKLNAFSYMDKSDSTTLKDMAKDLKIYV
TPINMYEENERLYDLKQKTALITDDEDRLNKIENIEDRQKKLESINEVFEKQAGIFFDKNYPEQALNYSD
DEKIFITRTILNDRDVLPANHELEDIVKEKRIKEAQISLNTVIGNRDISIESIEKESNFFADKLSNILDK
NNLSFDDVLENKHEGIEDSLKIDYYTSKLEVLRNAENTLEDYYDVQIKELFTDDEDYKAFNEVTDIKEKQ
QLIDFKTYHGTENTLEMLETGNFIPKYSDEDRKYITEQVKLLQEKEFKPNKNQHDKFVFGAIQKKLLNEY
DFDYSDNNDLKHLYQESNEVGDETSKDNIEEFYEGNDILVDKETYNNYNRKQQAYGLVNAGLDSMIFNFN
EIFRERMPKYINNQYKGKNHSKQRHELKNKRGMHL

  Protein domains


Predicted by InterproScan.

MobA/MobL family (MobA_MobL)

(17-216)

Nicking enzyme C-terminal middle helical domain (NES_C_h)

(265-359)


  Protein structure


Source ID Structure
AlphaFold DB A0A848EWD6


T4CP

ID   5209 GenBank   WP_030061486
Name   t4cp2_HTC99_RS00065_pLRSA417 insolico UniProt ID   _
Length   548 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 548 a.a.        Molecular weight: 62899.75 Da        Isoelectric Point: 5.9714

>WP_030061486.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Staphylococcaceae]
MTTLLGELESKATSINKKNLVIQDFDTKCPNRNIFVVGGPGSFKSAGYVIPNVIVKNQQSIVVTDPSGEV
YEKTANIKRMQGYDVRVVNFKNFLASDRQNFFDYIDKDSDCSIVAELIIKSAGDSKMNKDVWYKSSVGLL
NSLLLYAKYEFEPEKRTIGNIIKFIQNNKPNHDDEGSVELDNRFNELPKDHPARESYEFGFAVSEGRTRA
SIILTFVADLRNYIDNEIRSYTSDSDFDLRDVGLRKTIIYVMLPVLGNTVQSLSSLFFSQMFQQLYRLGD
ENGARLPVPVDFLLDEWPNIGAIPDYEETLATCRKYGISITTIVQSISQAVDKYNKDKANAIIGNHAVIL
CLGNVNNDTAKYIKEELGNATVEFETSSEGSSSGKDSRSKSSNKNVSYTGRALLNEDEISNMQQDKAILI
TKNRNPKIIKKLAYFKMFPNLTETYIAPQNEYKRKKNQSMIDKHNRLREEWDKKQEKIKQQKLEEYKEEQ
RQKELEEEQQAQEEQQNEEVKESKSKNEEQQEDKKDEQQKINDSKKAFYEKLKQKQAK

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(3-462)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 2416..14019

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTC99_RS00005 1..960 + 960 WP_030061463 replication initiator protein A -
HTC99_RS00010 1064..1252 - 189 WP_030061465 hypothetical protein -
HTC99_RS00015 1425..2399 + 975 WP_030061467 conjugative transfer protein TrsA -
HTC99_RS00020 2416..2733 + 318 WP_030061469 CagC family type IV secretion system protein virB2
HTC99_RS00025 2792..3199 + 408 WP_030061471 hypothetical protein trsC
HTC99_RS00030 3186..3869 + 684 WP_030061473 TrsD/TraD family conjugative transfer protein trsD
HTC99_RS00035 3890..5902 + 2013 WP_030061475 DUF87 domain-containing protein virb4
HTC99_RS00040 5914..7194 + 1281 WP_030061477 hypothetical protein trsF
HTC99_RS00045 7212..8288 + 1077 WP_030061480 CHAP domain-containing protein trsG
HTC99_RS00050 8298..8783 + 486 WP_000735570 TrsH/TraH family protein -
HTC99_RS00055 8799..10901 + 2103 WP_030061483 DNA topoisomerase III -
HTC99_RS00060 10917..11381 + 465 WP_000715271 hypothetical protein trsJ
HTC99_RS00065 11378..13024 + 1647 WP_030061486 VirD4-like conjugal transfer protein, CD1115 family -
HTC99_RS00070 13102..14019 + 918 WP_030061488 conjugal transfer protein TrbL family protein trsL
HTC99_RS00075 14036..14431 + 396 WP_000608969 single-stranded DNA-binding protein -
HTC99_RS00080 14488..15219 + 732 WP_000869299 hypothetical protein -
HTC99_RS00085 15256..15879 + 624 WP_030061493 hypothetical protein -
HTC99_RS00090 15895..16509 + 615 WP_030061494 hypothetical protein -
HTC99_RS00095 16578..16844 + 267 WP_030061495 hypothetical protein -
HTC99_RS00100 16930..18102 + 1173 WP_000195429 IS256-like element IS256 family transposase -

Host bacterium

ID   7919 GenBank   NZ_KJ922127
Plasmid name   pLRSA417 Incompatibility group   -
Plasmid size   39504 bp Coordinate of oriT [Strand]   36490..36525 [+]
Host baterium   Staphylococcus aureus strain 417

Cargo genes

Drug resistance gene   aac(6')-aph(2''), cfr
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA21