Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 107464
• Name: oriT_pCPS32
• Organism: Staphylococcus aureus
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NC_019141 (4303..4383 [+], 81 nt)
• oriT length: 81 nt
• IRs (inverted repeats): _
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 81 nt

>oriT_pCPS32
TTCAGCTGAAAATTTGCATATGAGTGTGCCTGCTTTCGTTAAGAAAAAGGCACAAGGGGCGAGATTGGTAGCACCGAAATT

oriT secondary structure

Predicted by RNAfold.

Relaxase

Rep_trans_HXF92_RS00010_pCPS32

• ID: 4997
• GenBank: WP_012579074
• Name: Rep_trans_HXF92_RS00010_pCPS32
• UniProt ID: E4Z838
• Length: 312 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 312 a.a.
• Molecular weight: 37253.28 Da
• Isoelectric Point: 6.7054

>WP_012579074.1 replication initiation factor domain-containing protein [Staphylococcus aureus]
MSENIQENHSKQGTSGEVTNYTRTGYSNSRLDAHFLCTSHPKLSFDGMTIVGNLNKNNAERLSKFMSIEP
QIRMWDILQTKFKAKALQEKVYIEYDKVKADSWDRRNMRVEFNPNKLSHDEMVWLKRNIINLMEDDGFTR
LDLAFDFEDDLSDYYAMSDKALKKTVFYGIDGKPETKYFGVRDSERFIRIYNKKKERKDNADIEIDSEHL
WRVEIELKRNMVDYWNNCFDDLHILKPAWATLESVREQAMVYLLLHEESAWGELHRNSRRKYKQLLQEIS
PIDLTNLMKLALKENEKQLQKQIDFWLSDFRF

Protein domains

Predicted by InterproScan.

• Replication initiation factor (Rep_trans) (138-283)

Protein structure

• AlphaFold DB: E4Z838

Host bacterium

• ID: 7899
• GenBank: NC_019141
• Plasmid name: pCPS32
• Incompatibility group: -
• Plasmid size: 5718 bp
• Coordinate of oriT [Strand]: 4303..4383 [+]
• Host baterium: Staphylococcus aureus

Cargo genes

• Drug resistance gene: vga(A)LC
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -