Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 107451 |
| Name | oriT_pRapFH23d  |
| Organism | Rhizobium acidisoli strain FH23 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP035002 (65724..65752 [+], 29 nt) |
| oriT length | 29 nt |
| IRs (inverted repeats) | 14..19, 24..29 (CGTCGC..GCGACG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 29 nt
>oriT_pRapFH23d
AGGGCGCAATATACGTCGCTGTCGCGACG
AGGGCGCAATATACGTCGCTGTCGCGACG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 4990 | GenBank | WP_128715647 |
| Name | traA_CO657_RS32780_pRapFH23d |
UniProt ID | _ |
| Length | 1545 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1545 a.a. Molecular weight: 170880.02 Da Isoelectric Point: 8.6519
>WP_128715647.1 Ti-type conjugative transfer relaxase TraA [Rhizobium acidisoli]
MAIMFVRAQVISRGAGRSIVSAAAYRHRARMIDEQAGTSFSYRGGASELMHEELALPGEIPAWLRSAISG
QSVARASEVLWNAVDAFEKRADAQLARELIIALPEELTRAENIALVRDFVRDNLTSKGMVADWVYHDKEG
NPHIHLMMTLRPMIEEGFGPKKVPVLSEEGKPLRVVTPDRPNGKIVYRVWAGDKETMKAWKIAWAETANR
HLALAGHEIRLDGRSYAEQGLGGIAQKHLGPEKAALARNGRELYFAPADLARRQEIADRLLAEPDLLLKQ
LGNERSTFDERDIAKALHRTVDDPGDFANIRARLMASDQLVILKPQEIDQETGKAAAPAVFTTRDILRIE
YDMAQSARVLSKRGGFGVSRRHVEAAIDRVESRDPKNPFRLDAEQVDAVHHVTGDSGIAAVVGLAGAGKS
TLLAAARLAWESQGRRVIGAALAGKAAEGLEDSSGITSRTIASWELAWANGRDALHRGDVLVIDEAGMVA
SQQMARVLKIAEEAEVKVILVGDAMQLQPIQAGAAFRAITERIGFAELAGVRRQREAWARDASRLFARGD
VEKGLDAYAQQGHLVEAGTREETIDRIVSDWAAARKQAIGRSISEGRDGHLRGDELLVLAHTNDDVKRLN
EALREVMAGEGALSDSRAFRTERGTRDFAVGDRIIFLENARFLEPRAKHSGPQYVKNGMLGTVVATGDKR
GDPLLSVLLDNGRKVVFSEDSYRNVDHGYAATIHKSQGATVDRTFVLATGMMDRHLTYVSMTRHRDRVDL
YAAKEDFEPRPEWGRKPRVDHAAGVTGELVETGEAKFRPEDKDADDSPYGDVRTDDGTVHRLWGVSLPKA
LEDAGISDGDTVTLRKDGVERVKVQVAIVDEATGQKRYEEREVDRNVWTASRIETAYVRRERIAQESHRP
EVFEQLVERLSRSGAKTTTLDFESEAGYRAQAQDFARRRGLDHLSLAAAEMEESLSRRWTWIAAKREQVE
KLWERASVALGFAIERERRVAYNEARTEPHIVSGASAGEAHYLIPPTTSFVRSVEADARRAQHSSPAWAE
REAVLRPLLDKIYRDPDAALVALNALASDAGTQPRRLADELAAAPDRLGRLRGSELIVDGRAARNERKVV
MAAVKELLPLARAHATEFRRNAERFELREQTRRSYMSLSIPALSERATARLMEIEAVRSRGGDDAYKTAF
ALAAEDRTIVQEIKAVSEALSARFGWSAFTAKADTVAERNIAERMPEDLTAGRREELTGLFEAVKRFAGE
QHLTERRDRSKVAAGASADLSKESGKENVTMPPMFAAVTEFKTSVDDEARLRALSNPLYRQQRPALANAA
TTVWRDPASVVGKIEELLGKGFAADRIAAAVTNDPAAYGALRGSDRLMDRMLASGRERKEVMRAVPEVAA
RLRSLGTAYANTLEAERQAIIEERRRMAVAIPSLSRAAEEALLHLTAEVKTNGRKPHVSATPLDSSIRRE
FADVSRALDERFGRNAIIRGDEDLISRVPPAQRPAFATMQDRLKVLQQTVRQESSEQIIAERRQRASQRS
RGIGL
MAIMFVRAQVISRGAGRSIVSAAAYRHRARMIDEQAGTSFSYRGGASELMHEELALPGEIPAWLRSAISG
QSVARASEVLWNAVDAFEKRADAQLARELIIALPEELTRAENIALVRDFVRDNLTSKGMVADWVYHDKEG
NPHIHLMMTLRPMIEEGFGPKKVPVLSEEGKPLRVVTPDRPNGKIVYRVWAGDKETMKAWKIAWAETANR
HLALAGHEIRLDGRSYAEQGLGGIAQKHLGPEKAALARNGRELYFAPADLARRQEIADRLLAEPDLLLKQ
LGNERSTFDERDIAKALHRTVDDPGDFANIRARLMASDQLVILKPQEIDQETGKAAAPAVFTTRDILRIE
YDMAQSARVLSKRGGFGVSRRHVEAAIDRVESRDPKNPFRLDAEQVDAVHHVTGDSGIAAVVGLAGAGKS
TLLAAARLAWESQGRRVIGAALAGKAAEGLEDSSGITSRTIASWELAWANGRDALHRGDVLVIDEAGMVA
SQQMARVLKIAEEAEVKVILVGDAMQLQPIQAGAAFRAITERIGFAELAGVRRQREAWARDASRLFARGD
VEKGLDAYAQQGHLVEAGTREETIDRIVSDWAAARKQAIGRSISEGRDGHLRGDELLVLAHTNDDVKRLN
EALREVMAGEGALSDSRAFRTERGTRDFAVGDRIIFLENARFLEPRAKHSGPQYVKNGMLGTVVATGDKR
GDPLLSVLLDNGRKVVFSEDSYRNVDHGYAATIHKSQGATVDRTFVLATGMMDRHLTYVSMTRHRDRVDL
YAAKEDFEPRPEWGRKPRVDHAAGVTGELVETGEAKFRPEDKDADDSPYGDVRTDDGTVHRLWGVSLPKA
LEDAGISDGDTVTLRKDGVERVKVQVAIVDEATGQKRYEEREVDRNVWTASRIETAYVRRERIAQESHRP
EVFEQLVERLSRSGAKTTTLDFESEAGYRAQAQDFARRRGLDHLSLAAAEMEESLSRRWTWIAAKREQVE
KLWERASVALGFAIERERRVAYNEARTEPHIVSGASAGEAHYLIPPTTSFVRSVEADARRAQHSSPAWAE
REAVLRPLLDKIYRDPDAALVALNALASDAGTQPRRLADELAAAPDRLGRLRGSELIVDGRAARNERKVV
MAAVKELLPLARAHATEFRRNAERFELREQTRRSYMSLSIPALSERATARLMEIEAVRSRGGDDAYKTAF
ALAAEDRTIVQEIKAVSEALSARFGWSAFTAKADTVAERNIAERMPEDLTAGRREELTGLFEAVKRFAGE
QHLTERRDRSKVAAGASADLSKESGKENVTMPPMFAAVTEFKTSVDDEARLRALSNPLYRQQRPALANAA
TTVWRDPASVVGKIEELLGKGFAADRIAAAVTNDPAAYGALRGSDRLMDRMLASGRERKEVMRAVPEVAA
RLRSLGTAYANTLEAERQAIIEERRRMAVAIPSLSRAAEEALLHLTAEVKTNGRKPHVSATPLDSSIRRE
FADVSRALDERFGRNAIIRGDEDLISRVPPAQRPAFATMQDRLKVLQQTVRQESSEQIIAERRQRASQRS
RGIGL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 5200 | GenBank | WP_054186343 |
| Name | traG_CO657_RS32765_pRapFH23d |
UniProt ID | _ |
| Length | 638 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 638 a.a. Molecular weight: 70459.52 Da Isoelectric Point: 9.4707
>WP_054186343.1 Ti-type conjugative transfer system protein TraG [Rhizobium acidisoli]
MIARGKPHPSLLVFAPVVVTAIAVYIAGWRWPGLAAGMPGRMEYWFLRAAPVPALLLGPLAGLLAVWALP
LHRRRPVAMTSLLCYLGIAAFYALREFARLGPSVQAGIITWDRALSYIDMVAVIGAVAGFLAVAVAARIS
VVVPDDIKRARRGTFGDADWLPMAAAAKLFPPDGEIVVGERYRVDKEIVHELPFDANDRSTWGQGGKAPL
LTYKQDFDSTHMLFFAGSGGYKTTSNVVPTALRYTGPLICLDPSTEVAPMVVEHRTHALGREVMVLDPTN
PIMGFNVLDGIETSRQKEEDIVGIAHMLLSESARFESSTGSYFQSQAHNLLTGLLAHVLLSPEYAGRRNL
RSLREIVSEPEPSVLAMLRDIQENSQSDFIRETLGVFTNMTEQTFSGVYSTASKDTQWLSLDNYAALVCG
KAFKPSDIVSGRKDVFLNIPASILRSYPGIGRVIIGSLINAMVQADGVFRRRALFMLDEVDLLGYMRVLE
EARDRGRKYGISIMLMYQSVGQLERHFGKDGATSWIEGCAFASYAAIKALDTARTVSAQCGEMTVEVKGS
SRNLGWDSRNGGTRKSESLSFQRRPLIMPHEITQSMRKDEQIIIVQGHRPIRCGRAIYFRRKEMDRVAKA
NRFVKPTL
MIARGKPHPSLLVFAPVVVTAIAVYIAGWRWPGLAAGMPGRMEYWFLRAAPVPALLLGPLAGLLAVWALP
LHRRRPVAMTSLLCYLGIAAFYALREFARLGPSVQAGIITWDRALSYIDMVAVIGAVAGFLAVAVAARIS
VVVPDDIKRARRGTFGDADWLPMAAAAKLFPPDGEIVVGERYRVDKEIVHELPFDANDRSTWGQGGKAPL
LTYKQDFDSTHMLFFAGSGGYKTTSNVVPTALRYTGPLICLDPSTEVAPMVVEHRTHALGREVMVLDPTN
PIMGFNVLDGIETSRQKEEDIVGIAHMLLSESARFESSTGSYFQSQAHNLLTGLLAHVLLSPEYAGRRNL
RSLREIVSEPEPSVLAMLRDIQENSQSDFIRETLGVFTNMTEQTFSGVYSTASKDTQWLSLDNYAALVCG
KAFKPSDIVSGRKDVFLNIPASILRSYPGIGRVIIGSLINAMVQADGVFRRRALFMLDEVDLLGYMRVLE
EARDRGRKYGISIMLMYQSVGQLERHFGKDGATSWIEGCAFASYAAIKALDTARTVSAQCGEMTVEVKGS
SRNLGWDSRNGGTRKSESLSFQRRPLIMPHEITQSMRKDEQIIIVQGHRPIRCGRAIYFRRKEMDRVAKA
NRFVKPTL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Host bacterium
| ID | 7886 | GenBank | NZ_CP035002 |
| Plasmid name | pRapFH23d | Incompatibility group | - |
| Plasmid size | 845857 bp | Coordinate of oriT [Strand] | 65724..65752 [+] |
| Host baterium | Rhizobium acidisoli strain FH23 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | gmd |
| Metal resistance gene | hupE2 |
| Degradation gene | bphC |
| Symbiosis gene | noeL |
| Anti-CRISPR | AcrIIA9, AcrIIA7 |