Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   107449
Name   oriT_pR997 in_silico
Organism   Proteus mirabilis strain R997
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_KY433363 (3518..3816 [+], 299 nt)
oriT length   299 nt
IRs (inverted repeats)      45..50, 52..57  (CAAACG..CGTTTG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 299 nt

>oriT_pR997
GCTCTGTTTGGCGGCGGATGACCTAGTCAAAAAAATCGAGACGCCAAACGTCGTTTGCATTCTGGCCTGAACTCGCCAAACGGTTTGTATCTTCATGACGATACGTCGTTTTAGGCGTTTTTAAGTGAAATCGGGCTGTATCCCTTGTCAGGTATGGGATTGCGCGAGTTGATTTATATCGAGACGCCAAACAGTGATTGTTACGGCAGTTTTACGTTTGGCGTTTCGATCCAAAAGCCAAACGGATAGTGGTTTTGGCTTTTGGGGTTAATTGGATGGGGAAATTGGTTTGGTAGAAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   4988 GenBank   WP_048609057
Name   mobH_HTL41_RS00140_pR997 insolico UniProt ID   A0A1H7RMG5
Length   716 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 716 a.a.        Molecular weight: 79771.82 Da        Isoelectric Point: 5.0941

>WP_048609057.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MFKNLFFQTKALPELSSQLDTEIPRYPPFLKGLPAASPEDLQSTQDELIAKLRQVLGFNLRDFQRLIQPC
IDHLAAYVHLLPASEHHHHSGAGGLLRHSLEVAFWAAQAAEGIIFVASGTPVEKKELEPRWRVAAALGGL
FHDIGKPVSDLSITDEDGRYQWNPFLETLSQWTTNNSIERYFIRWRDGRCKRHEQFSILVLNRVMTPELL
AWLTQPGPEILQAMLEAIGNTDPEHVLSKLVIEADQTSVQRDLKAQRISVDDNALGVPVERYLLDAMRRL
LASSQWLVNQRDARVWVRKSNQSTHLYLVWKSAAKDIIELLAKDKIPGIPRDPDTLADILIERGLATKST
SNERYESLAPEVLIKDDKPIWLAMLHIVEADLLFSSNVPSGVTLFSKPDWEATQQTQAEPQSRSSEHLDL
PEASSSIELSNSAESPSTKSSEQDDELRHASDVNHLQATENVPGDGCEKPNDSNDATISNNVNQHDADAF
NLPESLAWLPEASSALIMAGEQILIRYPDAVRPWCAPRKLLAELSRLDWLELDPANPTRKARTVTTNDGV
QEQGLLLKVSISKGLIALIDLSKQDAESVEPIQNEEASQRPSRTETANAQAKEPATRAERKQKPIAPNAN
SSTDLKHAQRQQVVNFVKDLPILLTDGNYPDVDHSADGIRVTIQTLRQVANEHGIPAGQLLRGISASDQC
QFDEGETVLFTAHAKR

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(39-350)


  Protein structure


Source ID Structure
AlphaFold DB A0A1H7RMG5


T4CP

ID   5197 GenBank   WP_048609054
Name   traD_HTL41_RS00145_pR997 insolico UniProt ID   _
Length   606 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 606 a.a.        Molecular weight: 67156.97 Da        Isoelectric Point: 6.5130

>WP_048609054.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MKENAYEMPWRTNYEAMAVAGWLVGATGAIAAEMLTELPPEPFWWMTGISSGMALYRLPEAYRLYKLQKG
LKGKPLAFMELSHLQKVMAKHPDELWLGYGFEWDQRHAQRAYEILKRDKQTLLNQGHGKQMGSTWIHGVE
PKEEDVYQPVGHTEGHTLIVGTTGAGKTRCFDAMITQAILRNEAVIIIDPKGDKELKDNAQRACIAAGSP
ERFVYFHPGFPEHSVRLNPLRNFNRGTEIASRIAALIPSETGADPFKAFGQMALNNIVQGLLLTSQRPDL
KTLRRFLEGGPEGLVVKAVTAWGEQVYPNFSVEIKRFTEKANTLAKQAMAMLLFYYERIQPVAANTDLEG
LLSMFEHDRTHYSKMVASLMPVLNMLTSSELGPLLSPIANDVDDSRLITDSGRIINNAQVAYIGLDSLTD
AMVGSAIGSLLLSDLTAVAGDRYNYGVENRPVNIFIDEAAEVVNDPFIQLLNKGRGAKMRCVIATQTFAD
FSARTGSEAKARQVLGNINNLIALRVMDAETQQYITDNLPKTRLQYIMQTQGMSSNSDSPALFTGNHGER
LMEEEGDMFPPQLLGQLPNLEYIAKLSGGRVIKGRIPILTSSTQAA

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(451-578)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(152-285)

  Protein structure



No available structure.



ID   5198 GenBank   WP_048608542
Name   traC_HTL41_RS00210_pR997 insolico UniProt ID   _
Length   799 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 799 a.a.        Molecular weight: 90174.75 Da        Isoelectric Point: 5.8177

>WP_048608542.1 MULTISPECIES: type IV secretion system protein TraC [Gammaproteobacteria]
MKASLYSGQRASELLPVLAYSDDEQLFFMEDQSVGFGFLCDPLPGGDESVADRVNVLLNNDWPKDTLLQF
GLYASPDIQTDLQLMMGLRHRQSDPLLRASIRKRADFLDGGTVQPIEESTQTQVRNFQLIVTCKLPLESP
IPTERELSRTSALRASFSQALTTVGFRVTEMTDRNWLAALSAQLNWGKDASWRNPSPIRSEADKPLREQV
LDYDRAIKVDSQGLMLGDYRVKTLSFKRLPERIWFGHAASFAGDMMTGSRGLRGSFLLNVTIHFPSAEAM
RSRLETKRQWAVNQAYGPMLKFVPVLAAKKKGFDVLFEALQEGDRPIRANMTLTLFSPTEEASISSVSNA
RTYFKELGFELMEDKYFCLPIFLNALPFGADRQAMNDLFRFKTMATRHVIPLLPLFADWKGTGTPVINFV
SRNGQIMSVSLYDSGSNYNCCIAAQSGSGKSFLVNEIISSYLSEGGQCWVIDVGRSYEKLCEVYDGEFLQ
FGRDSGICLNPFEIVEDYDEEADVLVGLLAAMAAPTQSLTDFQMANLKRQTRELWEKKGRAMLVDDVAEA
LKNHDDRRVQDVGEQLYPFTTQGEYGRFFNGHNNIRFKNRFTVLELEELKGRKHLQQVVLLQLIYQIQQE
MYLGERDRRKIVFIDEAWDLLTQGDVGKFIETGYRRFRKYGGSAVTVTQSVNDLYDSPTGKAIAENSANM
YLLGQKAETINALKKEGRLPLGEGGYEYLKTVHTVTGVYSEIFFITEMGTGIGRLIVDPFHKLLYSSRAE
DVNAIKQLTRKGLSVADAISQLLKERGYE

  Protein domains


Predicted by InterproScan.

TraC protein (TraC_F_IV)

(11-241)

Family of unknown function (DUF5934) (DUF5934)

(253-420)

TraG P-loop domain (P-loop_TraG)

(436-521)

TraG P-loop domain (P-loop_TraG)

(568-794)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 30347..54125

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTL41_RS00125 26326..27180 + 855 WP_000255863 restriction endonuclease -
HTL41_RS00130 27274..27555 + 282 WP_001067695 hypothetical protein -
HTL41_RS00135 27555..28028 + 474 WP_001145412 hypothetical protein -
HTL41_RS00140 28148..30298 + 2151 WP_048609057 MobH family relaxase -
HTL41_RS00145 30347..32167 + 1821 WP_048609054 conjugative transfer system coupling protein TraD virb4
HTL41_RS00150 32177..32737 + 561 WP_000546513 hypothetical protein -
HTL41_RS00155 32724..33359 + 636 WP_045611026 DUF4400 domain-containing protein tfc7
HTL41_RS00160 33386..33973 - 588 WP_085285720 plasmid-related protein -
HTL41_RS00420 33987..34226 - 240 WP_011788473 hypothetical protein -
HTL41_RS00165 34262..34543 + 282 WP_000433889 type IV conjugative transfer system protein TraL traL
HTL41_RS00170 34540..35166 + 627 WP_000667170 TraE/TraK family type IV conjugative transfer system protein traE
HTL41_RS00175 35150..36046 + 897 WP_048608549 type-F conjugative transfer system secretin TraK traK
HTL41_RS00180 36049..37338 + 1290 WP_085285719 TraB/VirB10 family protein traB
HTL41_RS00185 37413..37985 + 573 WP_139175592 type IV conjugative transfer system lipoprotein TraV traV
HTL41_RS00190 37982..38368 + 387 WP_048608546 TraA family conjugative transfer protein -
HTL41_RS00195 38589..39380 + 792 WP_048608544 type IV toxin-antitoxin system AbiEi family antitoxin -
HTL41_RS00200 39373..40311 + 939 WP_172689548 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
HTL41_RS00205 40443..41135 + 693 WP_001228924 DsbC family protein trbB
HTL41_RS00210 41135..43534 + 2400 WP_048608542 type IV secretion system protein TraC virb4
HTL41_RS00215 43527..43874 + 348 WP_048608540 hypothetical protein -
HTL41_RS00220 43858..44370 + 513 WP_172689549 S26 family signal peptidase -
HTL41_RS00225 44381..45505 + 1125 WP_048608537 TrbC family F-type conjugative pilus assembly protein traW
HTL41_RS00230 45489..46517 + 1029 WP_048608535 TraU family protein traU
HTL41_RS00235 46520..50212 + 3693 WP_004249370 conjugal transfer protein TraN traN
HTL41_RS00240 50671..52089 + 1419 WP_004249371 SIR2 family protein -
HTL41_RS00245 52086..54125 + 2040 WP_004249372 DUF87 domain-containing protein virb4
HTL41_RS00250 54534..55208 - 675 WP_048608563 endonuclease -
HTL41_RS00255 55250..56539 - 1290 WP_048608532 ISL3 family transposase -
HTL41_RS00260 56561..57067 - 507 WP_007146113 signal peptidase II -
HTL41_RS00265 57077..57973 - 897 WP_048608527 cation transporter -
HTL41_RS00270 58069..58476 + 408 WP_048608525 Cd(II)/Pb(II)-responsive transcriptional regulator -

Host bacterium

ID   7884 GenBank   NZ_KY433363
Plasmid name   pR997 Incompatibility group   -
Plasmid size   85368 bp Coordinate of oriT [Strand]   3518..3816 [+]
Host baterium   Proteus mirabilis strain R997

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -