Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 107435 |
| Name | oriT_IncI2  |
| Organism | Salmonella enterica strain STH21 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_LN623683 (7377..7429 [-], 53 nt) |
| oriT length | 53 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 53 nt
>oriT_IncI2
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 5172 | GenBank | WP_000338974 |
| Name | t4cp2_HXH36_RS00195_IncI2 |
UniProt ID | _ |
| Length | 652 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 652 a.a. Molecular weight: 73347.89 Da Isoelectric Point: 9.1391
>WP_000338974.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MDAKKTGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQECFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LEQKAKGHNVPDVLVSISAILKTSVPDGGKDLAAWMGQEIENRSWISDKTKSFFFKFMSAPDRTRGSIET
NFSSPLSIFSNPITAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
MDAKKTGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQECFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LEQKAKGHNVPDVLVSISAILKTSVPDGGKDLAAWMGQEIENRSWISDKTKSFFFKFMSAPDRTRGSIET
NFSSPLSIFSNPITAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 23269..46814
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HXH36_RS00130 | 18594..19247 | - | 654 | WP_015387348 | hypothetical protein | - |
| HXH36_RS00135 | 19259..20383 | - | 1125 | WP_000486719 | site-specific integrase | - |
| HXH36_RS00140 | 20718..20978 | - | 261 | WP_198965019 | pilus assembly protein | - |
| HXH36_RS00145 | 21373..22617 | - | 1245 | WP_015387351 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| HXH36_RS00150 | 22630..23265 | - | 636 | WP_000934978 | A24 family peptidase | - |
| HXH36_RS00155 | 23269..23697 | - | 429 | WP_001326801 | lytic transglycosylase domain-containing protein | virB1 |
| HXH36_RS00160 | 23818..24375 | - | 558 | WP_000095051 | type 4 pilus major pilin | - |
| HXH36_RS00165 | 24420..25529 | - | 1110 | WP_000974900 | type II secretion system F family protein | - |
| HXH36_RS00170 | 25520..27058 | - | 1539 | WP_001420475 | ATPase, T2SS/T4P/T4SS family | virB11 |
| HXH36_RS00175 | 27083..27577 | - | 495 | WP_000912551 | type IV pilus biogenesis protein PilP | - |
| HXH36_RS00180 | 27561..28883 | - | 1323 | WP_000454143 | type 4b pilus protein PilO2 | - |
| HXH36_RS00185 | 28922..30565 | - | 1644 | WP_176455541 | PilN family type IVB pilus formation outer membrane protein | - |
| HXH36_RS00190 | 30558..31076 | - | 519 | WP_015387353 | YfdA protein | virb4 |
| HXH36_RS00195 | 31123..33081 | - | 1959 | WP_000338974 | type IV secretory system conjugative DNA transfer family protein | - |
| HXH36_RS00200 | 33097..34152 | - | 1056 | WP_001059977 | P-type DNA transfer ATPase VirB11 | virB11 |
| HXH36_RS00205 | 34171..35310 | - | 1140 | WP_015387354 | TrbI/VirB10 family protein | virB10 |
| HXH36_RS00210 | 35300..36001 | - | 702 | WP_053882504 | TrbG/VirB9 family P-type conjugative transfer protein | - |
| HXH36_RS00215 | 36067..36801 | - | 735 | WP_000432282 | type IV secretion system protein | virB8 |
| HXH36_RS00220 | 36965..39322 | - | 2358 | WP_015387356 | VirB4 family type IV secretion system protein | virb4 |
| HXH36_RS00225 | 39328..39648 | - | 321 | WP_000362081 | VirB3 family type IV secretion system protein | virB3 |
| HXH36_RS00430 | 39719..40009 | - | 291 | WP_000865479 | conjugal transfer protein | - |
| HXH36_RS00235 | 40009..40593 | - | 585 | WP_001401693 | lytic transglycosylase domain-containing protein | virB1 |
| HXH36_RS00240 | 40614..41012 | - | 399 | WP_001708012 | hypothetical protein | - |
| HXH36_RS00245 | 41352..41789 | - | 438 | WP_015387358 | type IV pilus biogenesis protein PilM | - |
| HXH36_RS00250 | 41795..43030 | - | 1236 | WP_001419735 | TcpQ domain-containing protein | - |
| HXH36_RS00255 | 43033..43332 | - | 300 | WP_000835763 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| HXH36_RS00260 | 43399..44031 | - | 633 | WP_001419736 | hypothetical protein | - |
| HXH36_RS00265 | 44079..44888 | + | 810 | WP_001419737 | DUF5710 domain-containing protein | - |
| HXH36_RS00270 | 44935..45171 | - | 237 | WP_000750964 | EexN family lipoprotein | - |
| HXH36_RS00275 | 45175..45822 | - | 648 | WP_001419738 | type IV secretion system protein | - |
| HXH36_RS00280 | 45828..46814 | - | 987 | WP_001419739 | type IV secretion system protein | virB6 |
| HXH36_RS00285 | 46818..47075 | - | 258 | WP_000739144 | hypothetical protein | - |
| HXH36_RS00290 | 47072..47374 | - | 303 | WP_001360345 | hypothetical protein | - |
| HXH36_RS00295 | 47390..47641 | - | 252 | WP_015387362 | hypothetical protein | - |
| HXH36_RS00300 | 47638..47820 | - | 183 | WP_022540746 | hypothetical protein | - |
| HXH36_RS00305 | 47789..48451 | - | 663 | WP_001419740 | hypothetical protein | - |
| HXH36_RS00310 | 48462..48632 | - | 171 | WP_000550721 | hypothetical protein | - |
| HXH36_RS00315 | 48636..49079 | - | 444 | WP_000964840 | NfeD family protein | - |
| HXH36_RS00320 | 49152..50032 | - | 881 | Protein_62 | SPFH domain-containing protein | - |
| HXH36_RS00325 | 50078..50272 | - | 195 | WP_000049865 | DUF1187 family protein | - |
| HXH36_RS00330 | 50265..50771 | - | 507 | WP_001358485 | CaiF/GrlA family transcriptional regulator | - |
| HXH36_RS00335 | 51415..51774 | - | 360 | WP_015387332 | hypothetical protein | - |
Host bacterium
| ID | 7870 | GenBank | NZ_LN623683 |
| Plasmid name | IncI2 | Incompatibility group | IncI2 |
| Plasmid size | 62139 bp | Coordinate of oriT [Strand] | 7377..7429 [-] |
| Host baterium | Salmonella enterica strain STH21 |
Cargo genes
| Drug resistance gene | blaCTX-M-55 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |