Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   107425
Name   oriT_pOXA48-Pm in_silico
Organism   Proteus mirabilis strain Pm-Oxa48
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_KP025948 (49300..49404 [+], 105 nt)
oriT length   105 nt
IRs (inverted repeats)      32..37, 40..45  (GCCGGC..GCCGGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 105 nt

>oriT_pOXA48-Pm
AGTACGGGACAAGATGTGTTTTTGGTACACCGCCGGCACGCCGGCAGTGACGCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   4964 GenBank   WP_004206924
Name   Relaxase_HTE39_RS00335_pOXA48-Pm insolico UniProt ID   A0A2V4FJ40
Length   659 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 659 a.a.        Molecular weight: 75087.84 Da        Isoelectric Point: 10.0285

>WP_004206924.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFAGIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQLRSESDDKFNPK

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(86-334)


  Protein structure


Source ID Structure
AlphaFold DB A0A2V4FJ40


T4CP

ID   5167 GenBank   WP_004206885
Name   t4cp2_HTE39_RS00015_pOXA48-Pm insolico UniProt ID   _
Length   695 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 695 a.a.        Molecular weight: 79538.91 Da        Isoelectric Point: 4.8317

>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 52202..71398

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTE39_RS00315 47286..47597 + 312 WP_004187333 hypothetical protein -
HTE39_RS00320 47730..48266 + 537 WP_036970776 hypothetical protein -
HTE39_RS00325 48767..49132 - 366 WP_015586046 hypothetical protein -
HTE39_RS00330 49408..49725 + 318 WP_004206923 plasmid mobilization protein MobA -
HTE39_RS00335 49712..51691 + 1980 WP_004206924 TraI/MobA(P) family conjugative relaxase -
HTE39_RS00340 51705..52205 + 501 WP_004206925 DotD/TraH family lipoprotein -
HTE39_RS00345 52202..52981 + 780 WP_004187315 type IV secretory system conjugative DNA transfer family protein traI
HTE39_RS00350 52992..54155 + 1164 WP_004206926 plasmid transfer ATPase TraJ virB11
HTE39_RS00355 54145..54405 + 261 WP_004187310 IcmT/TraK family protein traK
HTE39_RS00440 54430..57735 + 3306 WP_220381329 LPD7 domain-containing protein -
HTE39_RS00365 57659..58213 + 555 WP_011154467 hypothetical protein traL
HTE39_RS00370 58214..58411 - 198 WP_004187464 Hha/YmoA family nucleoid-associated regulatory protein -
HTE39_RS00375 58398..58808 - 411 WP_004187465 H-NS family nucleoid-associated regulatory protein -
HTE39_RS00380 58807..59589 + 783 WP_004206929 DotI/IcmL family type IV secretion protein traM
HTE39_RS00385 59598..60749 + 1152 WP_015062843 DotH/IcmK family type IV secretion protein traN
HTE39_RS00390 60761..62110 + 1350 WP_036970781 conjugal transfer protein TraO traO
HTE39_RS00395 62122..62826 + 705 WP_004206933 conjugal transfer protein TraP traP
HTE39_RS00400 62850..63380 + 531 WP_004187478 conjugal transfer protein TraQ traQ
HTE39_RS00405 63397..63786 + 390 WP_011154472 DUF6750 family protein traR
HTE39_RS00410 63832..64326 + 495 WP_004187480 hypothetical protein -
HTE39_RS00415 64323..67373 + 3051 WP_011154474 conjugative transfer protein traU
HTE39_RS00420 67370..68578 + 1209 WP_004187486 conjugal transfer protein TraW traW
HTE39_RS00425 68575..69225 + 651 WP_004187488 hypothetical protein -
HTE39_RS00430 69218..71398 + 2181 WP_004187492 DotA/TraY family protein traY
HTE39_RS00435 71401..72054 + 654 WP_004206935 hypothetical protein -

Host bacterium

ID   7860 GenBank   NZ_KP025948
Plasmid name   pOXA48-Pm Incompatibility group   IncL/M
Plasmid size   72127 bp Coordinate of oriT [Strand]   49300..49404 [+]
Host baterium   Proteus mirabilis strain Pm-Oxa48

Cargo genes

Drug resistance gene   blaOXA-48, blaCTX-M-14b, aph(3'')-Ib, aph(3')-VIb, aph(6)-Id
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -