Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   107365
Name   oriT_pLG42 in_silico
Organism   Lactococcus garvieae strain IPLA 31405
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_KM007160 (34417..34553 [+], 137 nt)
oriT length   137 nt
IRs (inverted repeats)      21..28, 42..49  (AAAAGGGG..CCCCTTTT)
 25..30, 39..44  (GGGGAA..TTCCCC)
Location of nic site      104..105
Conserved sequence flanking the
  nic site    		  GCTTGCAGTA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 137 nt

>oriT_pLG42
CGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCAACATTGTAATACAAGAACGAAGTGCTTTGTATTACAATGTGATAGCTTGCAGTATTTATGGTTTTATATAGTCTATTTTGTT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   4913 GenBank   WP_004256101
Name   Relaxase_HTD20_RS00210_pLG42 insolico UniProt ID   A0A0D4CBU5
Length   410 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 410 a.a.        Molecular weight: 47671.28 Da        Isoelectric Point: 8.6735

>WP_004256101.1 relaxase/mobilization nuclease domain-containing protein [Lactococcus garvieae]
MATIAKISNGASAASALNYALGQDRPMHEKTEQWLQDHQLERPVELTNCRAVAVGGTNGIDPFIAKEQFD
VVRQLHNQTKESNQVLRITQSFALDELNPKVQKDWQKANDLGVELAENLYPNHQSAVYTHLDGKNHVLHN
HIIVNKVNLETGKKLREQKGESVQRAREMNDRLASRENWHILEPPKERQTETEKELIAKNEYSYMNDLRE
RINKSLQDVSVSSYETFKERLSDNGVILSERGQTFSYAFLDANNKQRRARETRLGSDFGRETILHELENR
ARQNEFRAVEQGEPAITPLERDTQQRESEIVSLEQAIEPRKSEALKRESKINRFIDTIKQFAGRVPELTQ
RVTRKLKQTKEKILDDFERRFSKDMKNYEQEQQKSLEKQANRDVQSEKKPTKDHDRGMSR

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(9-280)


  Protein structure


Source ID Structure
AlphaFold DB A0A0D4CBU5


Host bacterium

ID   7802 GenBank   NZ_KM007160
Plasmid name   pLG42 Incompatibility group   -
Plasmid size   42459 bp Coordinate of oriT [Strand]   34417..34553 [+]
Host baterium   Lactococcus garvieae strain IPLA 31405

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA7