Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   107364
Name   oriT_pAF14 in_silico
Organism   Lactococcus cremoris
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NC_019350 (14344..14419 [+], 76 nt)
oriT length   76 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 76 nt

>oriT_pAF14
CGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCTTTTCAAGCCACATTGTAATACAAGAACGAAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   4912 GenBank   WP_015062877
Name   Relaxase_HS977_RS00010_pAF14 insolico UniProt ID   I6TDG4
Length   410 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 410 a.a.        Molecular weight: 47733.39 Da        Isoelectric Point: 8.1264

>WP_015062877.1 relaxase/mobilization nuclease domain-containing protein [Lactococcus cremoris]
MATIAKISNGASAASALNYALGQDRPMHEKTEQWLQDHQLERPVELTNCRAVAVGGTNGIDPFIAKEQFD
VIRQLHNQTKESNQVLRITQSFALDELNPKVQKDWQKANDLGVELAENLYPNHQSAVYTHLDGKNHVLHN
HIIVNKVNLETGNKLREQKGESVQRAREMNDRLASRENWHILEPPKERQTETEKELIAKNEYSYMDDLRE
RINKSLQDVSVISYEAFKERLSDNGVILSERGQTFSYAFLDANNKQRRARETRLGSDFGRETILHELENR
ARQNEFRAVEQREPAITPLERDTQQRESEIVSLEQAIEPRKSEALKRESKINRFIDTIKQLAGRVPELTQ
RVTRKLKQTKEKILDDFERRFSKDMKNYEQEQQKSLEKQANRDVQSEKKPTKDHDRGMSR

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(9-280)


  Protein structure


Source ID Structure
AlphaFold DB I6TDG4


Host bacterium

ID   7801 GenBank   NC_019350
Plasmid name   pAF14 Incompatibility group   -
Plasmid size   14419 bp Coordinate of oriT [Strand]   14344..14419 [+]
Host baterium   Lactococcus cremoris

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -