Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   107360
Name   oriT_pNs15sousui29 in_silico
Organism   Lactococcus garvieae strain Ns15sousui29
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_LC316981 (4872..4909 [+], 38 nt)
oriT length   38 nt
IRs (inverted repeats)      1..7, 19..25  (ACACCAC..GTGGTGT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 38 nt

>oriT_pNs15sousui29
ACACCACCCAATTTTGGAGTGGTGTGTAAGTGCGCATT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   4911 GenBank   WP_043736840
Name   mobQ_HXH08_RS00015_pNs15sousui29 insolico UniProt ID   A0A224AWL5
Length   680 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 680 a.a.        Molecular weight: 79209.34 Da        Isoelectric Point: 6.6675

>WP_043736840.1 MULTISPECIES: MobQ family relaxase [Lactococcus]
MAIFHMNFSNISAGKGRSAVASASYRSGEKLYSEMENKTYFYDRSVMPESFILLPENAPEWAKDRQKLWN
EVEAVDRKVNSRYAKEFNVALPIELSEDEQKELLTEYVQKIFVDKGMVADVAIHRDHDENPHAHVMLTNR
PFNADGSWGQKAKKEYILDENGNKTYTANGHARSRKIWLVDWDKVGKVEEWRKAWADHVNSVFKEKNIDE
RISEKTLEAQGVNDIATQHVGVTGNRDERAEFNKLVLENRQHKAELENLDEKINNELKVKQLKNFYSFNE
KKVIAELSKELHTFIDLEHLEEKNKMLFNWKNSVLIKQIVGKDVSEELNKVSQQEFSLDNANRLIDKVVD
RSIKAMYPTVDSNAFSMPEKRQLIRETESENKVFTGAELDDRLSMIRADSLNRQIVALTKRPFTSLTMLS
KQEKYHIDNINNVLAYQGYNYENIEMSHGKILRNYESEEFSKELDIIQRSLKQINSIEEVRKVVEQQYSV
VLGTIFPDSELNKLSLVEKEKTYNAVIYFNPTIKKLTQSELENIVKEPPILFSTKEHNQGLSYLAGRIRL
EDINNKHLTRVLKFEGTKKLFIGEAKADKNIDPELLKEATNRNDKQSYKNEFYRDKNMENYQSVAYSDTS
PAEYMNRLFSGVLISVLYNNENQHKKGLEEVEWDMQRKHREQNKSGGLSR

  Protein domains


Predicted by InterproScan.

Nicking enzyme C-terminal middle helical domain (NES_C_h)

(276-383)

MobA/MobL family (MobA_MobL)

(17-237)


  Protein structure


Source ID Structure
AlphaFold DB A0A224AWL5


Host bacterium

ID   7797 GenBank   NZ_LC316981
Plasmid name   pNs15sousui29 Incompatibility group   -
Plasmid size   30639 bp Coordinate of oriT [Strand]   4872..4909 [+]
Host baterium   Lactococcus garvieae strain Ns15sousui29

Cargo genes

Drug resistance gene   -
Virulence gene   ugd
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA21