Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   107353
Name   oriT_pNUF18 in_silico
Organism   Lactococcus garvieae strain NUF18
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_LC316979 (4872..4909 [+], 38 nt)
oriT length   38 nt
IRs (inverted repeats)      1..7, 19..25  (ACACCAC..GTGGTGT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 38 nt

>oriT_pNUF18
ACACCACCCAATTTTGGAGTGGTGTGTAAGTGCGCATT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   4905 GenBank   WP_043736840
Name   mobQ_HXH21_RS00015_pNUF18 insolico UniProt ID   A0A224AWL5
Length   680 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 680 a.a.        Molecular weight: 79209.34 Da        Isoelectric Point: 6.6675

>WP_043736840.1 MULTISPECIES: MobQ family relaxase [Lactococcus]
MAIFHMNFSNISAGKGRSAVASASYRSGEKLYSEMENKTYFYDRSVMPESFILLPENAPEWAKDRQKLWN
EVEAVDRKVNSRYAKEFNVALPIELSEDEQKELLTEYVQKIFVDKGMVADVAIHRDHDENPHAHVMLTNR
PFNADGSWGQKAKKEYILDENGNKTYTANGHARSRKIWLVDWDKVGKVEEWRKAWADHVNSVFKEKNIDE
RISEKTLEAQGVNDIATQHVGVTGNRDERAEFNKLVLENRQHKAELENLDEKINNELKVKQLKNFYSFNE
KKVIAELSKELHTFIDLEHLEEKNKMLFNWKNSVLIKQIVGKDVSEELNKVSQQEFSLDNANRLIDKVVD
RSIKAMYPTVDSNAFSMPEKRQLIRETESENKVFTGAELDDRLSMIRADSLNRQIVALTKRPFTSLTMLS
KQEKYHIDNINNVLAYQGYNYENIEMSHGKILRNYESEEFSKELDIIQRSLKQINSIEEVRKVVEQQYSV
VLGTIFPDSELNKLSLVEKEKTYNAVIYFNPTIKKLTQSELENIVKEPPILFSTKEHNQGLSYLAGRIRL
EDINNKHLTRVLKFEGTKKLFIGEAKADKNIDPELLKEATNRNDKQSYKNEFYRDKNMENYQSVAYSDTS
PAEYMNRLFSGVLISVLYNNENQHKKGLEEVEWDMQRKHREQNKSGGLSR

  Protein domains


Predicted by InterproScan.

Nicking enzyme C-terminal middle helical domain (NES_C_h)

(276-383)

MobA/MobL family (MobA_MobL)

(17-237)


  Protein structure


Source ID Structure
AlphaFold DB A0A224AWL5


Host bacterium

ID   7790 GenBank   NZ_LC316979
Plasmid name   pNUF18 Incompatibility group   -
Plasmid size   30643 bp Coordinate of oriT [Strand]   4872..4909 [+]
Host baterium   Lactococcus garvieae strain NUF18

Cargo genes

Drug resistance gene   -
Virulence gene   ugd
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA21