Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   107342
Name   oriT_p7121-IMP in_silico
Organism   Klebsiella oxytoca strain 7121
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_KX784502 (11335..11424 [-], 90 nt)
oriT length   90 nt
IRs (inverted repeats)      34..41, 44..51  (AGTGCGAT..ATCGCACT)
Location of nic site      62..63
Conserved sequence flanking the
  nic site    		  GGTGTATAGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 90 nt

>oriT_p7121-IMP
TATTTCATTTTTTGTTTTTTTAATCAGTTAGTTAGTGCGATTCATCGCACTGCGTTAGGTGTATAGCAGGTTAAGGGCTAAAAAATAATC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   4894 GenBank   WP_024561907
Name   mobF_HTJ17_RS00100_p7121-IMP insolico UniProt ID   H1ZMV6
Length   1072 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1072 a.a.        Molecular weight: 118896.59 Da        Isoelectric Point: 6.4636

>WP_024561907.1 MULTISPECIES: MobF family relaxase [Enterobacterales]
MLDITTITRIDIASVVGYYADAKDDYYSKDSSFTAWHGQGAEALGLSGEVSSQRFKELLVGEIDPFTQMK
RSSGDATKERLGYDLTFSAPKGVSMQALIHGDKAIIAAHEKAVAAAVQEAEKLAQARTTHAGKTITQNTG
NLVVASFRHETSRALDPELHTHAFVMNMTQRGDGQWRALKNDELMRAKMHLGDVYKQELALELTKAGYEL
RYNNKNNTFDMAHFTDEQIRGFSRRSAQIEEGLAAMGLTRETADAQTKSRVSMATRDKKTDDYSREDIHK
EWVNRANELGIDFNDLSWAGKGKDGPQTSMNTVPNFTSPEVKADRAIQFALKSLSERDASFDQSRLLAVA
NKKAIGQASKQDIEAAYYRAVAKGAIIEGEARYTTTTDQRSKDAPPLPAMTKAEWQAQLTDKKGMNNDQA
KALVAEGIKSGRLKKTSHRVTTVEGIRVEKAVLNIEARGRGKVIAALTSEQVTAQLVGKTLKAEQQKSVV
DICTTTDRFVAAHGFAGTGKSYMTMSAKAVLESQGFNVTALAPYGTQKKSLEDEGMPARTVAAFVKAKDK
KIDEKSVVFIDEAGVIPARQMKVLMETIEKAGARAVFLGDTSQTKAVEAGKPFEQLISAGMQTSYMKDIQ
RQKNDVLLEAVKLAAEGHISGSLARLSNISAEKDQDKRLEAVANRWLSFSPEQREGTLIISGTNESRVIL
NSQIREALQLQGKGVEVNFLERVDSTQAERRDSKYYQVGQIIIPEKDYKNGLQRGESYRVLDTGPGNLLT
VAGSDGQKISFSPRTHKNLSVYTSVAAELAVGDKVMVTRNDKTLDVANGDRFTVTGTTEKGTFTLTNAKG
REIELDQKQAAYLSYAYATTVHKAQGLTCDRVLFNIDTRSLTTSKDVFYVGISRARHEVEIFTDDKKRLL
SSASRNSPKTTASEIDRFLGMEERYKDVNRSYGHSDGQQEKSYGHDAGHQTEEKENTMKNATEAAHDGPD
TSGTENTSQDLRQREQEEVNAAIRYEQRQEATPEMEANPYHDYERYADEGPDYGDMQDYSTYEEYERARD
VELPQHEHENHQHDHDKEGYGL

  Protein domains


Predicted by InterproScan.

TrwC relaxase (TrwC)

(16-288)

AAA domain (AAA_30)

(480-656)

UvrD-like helicase C-terminal domain (UvrD_C_2)

(855-899)


  Protein structure


Source ID Structure
AlphaFold DB H1ZMV6


T4CP

ID   5094 GenBank   WP_032493179
Name   t4cp2_HTJ17_RS00095_p7121-IMP insolico UniProt ID   _
Length   510 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 510 a.a.        Molecular weight: 57912.96 Da        Isoelectric Point: 9.5127

>WP_032493179.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MTEQERGLIFLCSLLGPIPFVWFITAKFVYGINSETAHYLVPYLLKNTFNLWPLWVAIIAGAVIGLCGFI
GFIIYDKGRVYKGPRFKRVLRGTKLVRASSLAEKTKERDHKQLTVAGVPVPTEYETLHFSIGGATGTGKS
TIIKEAIYSSLKRGTDKRIVLDPNGEYVETFYRPGDVILSPYDKRSPGWSMFNEIRDDFDYDLYAVSIIQ
ESKERSAEEWYRYGRLIYREVSKKLLTLKRSPTMEEVMHWCTIVENEKLKEFVKGTPAEALFIKGAERAT
GSARFVLGDKLAPHLKMPAGDFSLRDWLEDDKPGTLFITWQEQMKKSLNPLISCWLDIMFSSLLGMGKRD
QRTLFFIDELESLEYLPNLQDLLTKGRKSGASVWAGYQTYSQLIERYGINIAETLLGSMRSGIVMGSSRL
GKETLEQMSRALGEIEGEVEHKQGNPFQAGIGNRPRIETRTVRAVTPTEISELKNLTGYVYFPGDLPVGK
FKTKHVQYTRKNPVPGIIMR

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(113-492)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 28005..38281

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTJ17_RS00145 23059..23682 - 624 WP_003155741 recombinase family protein -
HTJ17_RS00150 23744..24961 - 1218 WP_006122485 TniQ family protein -
HTJ17_RS00155 24958..25866 - 909 WP_003155746 TniB family NTP-binding protein -
HTJ17_RS00160 25869..27548 - 1680 WP_000179844 Mu transposase C-terminal domain-containing protein -
HTJ17_RS00165 27623..27688 - 66 Protein_33 EAL domain-containing protein -
HTJ17_RS00170 27688..28008 - 321 Protein_34 phospholipase D-like domain-containing protein -
HTJ17_RS00175 28005..29006 - 1002 WP_001076634 ATPase, T2SS/T4P/T4SS family virB11
HTJ17_RS00180 29057..30190 - 1134 WP_000101920 type IV secretion system protein VirB10 virB10
HTJ17_RS00185 30190..31077 - 888 WP_000758230 TrbG/VirB9 family P-type conjugative transfer protein virB9
HTJ17_RS00190 31088..31783 - 696 WP_001208352 type IV secretion system protein virB8
HTJ17_RS00300 31794..31925 - 132 WP_032493177 hypothetical protein -
HTJ17_RS00195 32002..33039 - 1038 WP_014014948 type IV secretion system protein virB6
HTJ17_RS00200 33056..33298 - 243 WP_000858958 EexN family lipoprotein -
HTJ17_RS00205 33308..34009 - 702 WP_014014949 type IV secretion system protein virB5
HTJ17_RS00210 34025..36598 - 2574 WP_032493176 VirB4 family type IV secretion/conjugal transfer ATPase virb4
HTJ17_RS00215 36599..36916 - 318 WP_000462629 VirB3 family type IV secretion system protein virB3
HTJ17_RS00220 36956..37297 - 342 WP_001121032 TrbC/VirB2 family protein virB2
HTJ17_RS00225 37499..38281 - 783 WP_024562119 lytic transglycosylase domain-containing protein virB1
HTJ17_RS00230 38345..38698 + 354 WP_225988093 H-NS family nucleoid-associated regulatory protein -
HTJ17_RS00235 38702..39052 + 351 WP_024562120 hypothetical protein -
HTJ17_RS00290 39031..39387 + 357 WP_227648693 hypothetical protein -
HTJ17_RS00245 39391..39699 + 309 WP_000733764 hypothetical protein -
HTJ17_RS00250 39984..40169 + 186 WP_024562121 ribbon-helix-helix domain-containing protein -
HTJ17_RS00255 40166..40774 - 609 WP_000275218 recombinase family protein -
HTJ17_RS00260 40961..41293 - 333 WP_045723726 hypothetical protein -
HTJ17_RS00265 41365..41559 - 195 WP_050136673 hypothetical protein -
HTJ17_RS00270 41646..42293 - 648 WP_032493173 hypothetical protein -

Host bacterium

ID   7778 GenBank   NZ_KX784502
Plasmid name   p7121-IMP Incompatibility group   IncN3
Plasmid size   42461 bp Coordinate of oriT [Strand]   11335..11424 [-]
Host baterium   Klebsiella oxytoca strain 7121

Cargo genes

Drug resistance gene   blaIMP-1, aadA6
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -