Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 107332 |
| Name | oriT_pE20-qnrS  |
| Organism | Klebsiella aerogenes strain E20 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_MG288684 (40744..40825 [+], 82 nt) |
| oriT length | 82 nt |
| IRs (inverted repeats) | 27..32, 35..40 (GCCACC..GGTGGC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 82 nt
>oriT_pE20-qnrS
CGGGACAGGATATGTTTTGTATTACCGCCACCACGGTGGCAGCAATCCAAAACTCCTGGTCAGGGCGAAGCCCCGACACCCC
CGGGACAGGATATGTTTTGTATTACCGCCACCACGGTGGCAGCAATCCAAAACTCCTGGTCAGGGCGAAGCCCCGACACCCC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 4884 | GenBank | WP_032440569 |
| Name | Relaxase_HTP56_RS00270_pE20-qnrS |
UniProt ID | A0A7G3L4G1 |
| Length | 642 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 642 a.a. Molecular weight: 74384.24 Da Isoelectric Point: 8.3203
>WP_032440569.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MNAIIPPKRRDRKSSFVQLVAYVSVRDDVPLKDELKEDERFQRPSRSRQAIFDRLVDYMNRSEGTIIEQI
SDMTPEGDIRCSVDGVICQHNLMSVETAAMEMNSIAMQNTHVKDAVYHYILSWQEDENPSDDQVFDSVKH
SLKRLGMEEHQYVAAIHRDTDNLHVHIAANRVHPVSYRAANVWNDADKLQRTCRELELKHGFKVDNGSWV
RDADNNIVRARKGYRNAPRSARQLEHFSDKESLHHFAVQHVRKPVNAMFREKNASWEKLHQVMNDAGLML
ETSGKGLIVRDVCGDEKLAVKSSRVHPGMTLSRLEPALGTFVPSPRSAEMVKQSVQTSYSDKLHVRDRGA
RAERRQARADARIDLRERYEKYRNAWKKPDLHAAERFRAVSAAFKEQKTHIRDNEKDAHMRKLMYHIVEF
EREKSMAALRIALKAERQKLYDEGMMRPLTYRAWTEQQALHGDQAALSQLRGWSYRQNRKNRTPQASDNI
IYCAPADDTSLLKADGYEARLHRDGAVVYSRQGQDAIIDRGDRVEVINPYDDGDLNTRIAVDMVAWKSGE
RVEFSRDGSFAYAAGDAVAAHNLHHRDSIIVPSDREQYDYTREKFNQLKGRYTDSEREALRQQYDDRYYD
DVRHNDDSSYKP
MNAIIPPKRRDRKSSFVQLVAYVSVRDDVPLKDELKEDERFQRPSRSRQAIFDRLVDYMNRSEGTIIEQI
SDMTPEGDIRCSVDGVICQHNLMSVETAAMEMNSIAMQNTHVKDAVYHYILSWQEDENPSDDQVFDSVKH
SLKRLGMEEHQYVAAIHRDTDNLHVHIAANRVHPVSYRAANVWNDADKLQRTCRELELKHGFKVDNGSWV
RDADNNIVRARKGYRNAPRSARQLEHFSDKESLHHFAVQHVRKPVNAMFREKNASWEKLHQVMNDAGLML
ETSGKGLIVRDVCGDEKLAVKSSRVHPGMTLSRLEPALGTFVPSPRSAEMVKQSVQTSYSDKLHVRDRGA
RAERRQARADARIDLRERYEKYRNAWKKPDLHAAERFRAVSAAFKEQKTHIRDNEKDAHMRKLMYHIVEF
EREKSMAALRIALKAERQKLYDEGMMRPLTYRAWTEQQALHGDQAALSQLRGWSYRQNRKNRTPQASDNI
IYCAPADDTSLLKADGYEARLHRDGAVVYSRQGQDAIIDRGDRVEVINPYDDGDLNTRIAVDMVAWKSGE
RVEFSRDGSFAYAAGDAVAAHNLHHRDSIIVPSDREQYDYTREKFNQLKGRYTDSEREALRQQYDDRYYD
DVRHNDDSSYKP
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7G3L4G1 |
T4CP
| ID | 5081 | GenBank | WP_032440593 |
| Name | trbC_HTP56_RS00410_pE20-qnrS |
UniProt ID | _ |
| Length | 730 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 730 a.a. Molecular weight: 82679.78 Da Isoelectric Point: 6.3201
>WP_032440593.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacterales]
MQQKQKPVDAARLQRQVHQSMVEEMAYRTGPLLLAIIAIFGAAIVWPVLLWAAIPVMLVWVPMFMLNEFR
MPARMPMDMDRLDPSTETMRPGKLLGFLPVTKLNRHMEKAAGVFYTGYERTLDGGREIWLRMDDLTRHLI
LMATTGAGKTEMLLGFVLNALCWAKGLIFSDGKAQNDVWFAVASLSRRFGREDDLRLLNYITGGQSRSQR
LLENDKSRQQSNNTNPFALAQETYITQLMDSMMPEAGNDRTWQEKAKGMNQVLTKALIYKSRKEQKVMSQ
RLIQEYLSLDKFAELYLQAEAEEWHEEIRTGLRNYLSTSVPGFDMSLITKPSEWSQEARNQHGYLTSQYN
RMLSLFSDTYGHVFSSGAGDIDLRDVVHNDRILVILIPALELSASEALTLGRLTTSQLAMILSQDLGERM
EGKAEDILVIKKFRDRFPFLWLADEVGSYYSDTIGQLATQVRSLGYALVLAGQDLQKLKTAVGDKLWTLL
GNMFTRIFGTIIDPTETAEFAAKSAGMEYQAIQDSMEYRAGAIGGSYADTGRVTVQEKPKLPFDELQALN
QGEQATVFKGAVIRNNALYIADDDKITDKDVRINRFVEVEWPTYDSLSHLLPAKVRRDRPRPASINRIMA
IVKNDKIRHAPDSMIIQDGVLYAIADEAMYFDEVAPTPPNTVQRATQLLSLAAAVLTETRGRYRVEYPEP
EKLSVKKDELEKYQHIHQSAVTQQADGFSY
MQQKQKPVDAARLQRQVHQSMVEEMAYRTGPLLLAIIAIFGAAIVWPVLLWAAIPVMLVWVPMFMLNEFR
MPARMPMDMDRLDPSTETMRPGKLLGFLPVTKLNRHMEKAAGVFYTGYERTLDGGREIWLRMDDLTRHLI
LMATTGAGKTEMLLGFVLNALCWAKGLIFSDGKAQNDVWFAVASLSRRFGREDDLRLLNYITGGQSRSQR
LLENDKSRQQSNNTNPFALAQETYITQLMDSMMPEAGNDRTWQEKAKGMNQVLTKALIYKSRKEQKVMSQ
RLIQEYLSLDKFAELYLQAEAEEWHEEIRTGLRNYLSTSVPGFDMSLITKPSEWSQEARNQHGYLTSQYN
RMLSLFSDTYGHVFSSGAGDIDLRDVVHNDRILVILIPALELSASEALTLGRLTTSQLAMILSQDLGERM
EGKAEDILVIKKFRDRFPFLWLADEVGSYYSDTIGQLATQVRSLGYALVLAGQDLQKLKTAVGDKLWTLL
GNMFTRIFGTIIDPTETAEFAAKSAGMEYQAIQDSMEYRAGAIGGSYADTGRVTVQEKPKLPFDELQALN
QGEQATVFKGAVIRNNALYIADDDKITDKDVRINRFVEVEWPTYDSLSHLLPAKVRRDRPRPASINRIMA
IVKNDKIRHAPDSMIIQDGVLYAIADEAMYFDEVAPTPPNTVQRATQLLSLAAAVLTETRGRYRVEYPEP
EKLSVKKDELEKYQHIHQSAVTQQADGFSY
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 49934..71867
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HTP56_RS00280 | 45081..45485 | - | 405 | WP_223289767 | molybdopterin-dependent oxidoreductase | - |
| HTP56_RS00285 | 45568..46497 | - | 930 | WP_032440581 | hypothetical protein | - |
| HTP56_RS00290 | 46557..47399 | - | 843 | WP_032440572 | helix-turn-helix domain-containing protein | - |
| HTP56_RS00295 | 48009..48236 | + | 228 | WP_032440573 | hypothetical protein | - |
| HTP56_RS00300 | 48587..48955 | + | 369 | WP_126523558 | hypothetical protein | - |
| HTP56_RS00305 | 48968..49447 | + | 480 | WP_032440574 | lytic transglycosylase domain-containing protein | - |
| HTP56_RS00310 | 49473..49937 | + | 465 | WP_225546581 | DotD/TraH family lipoprotein | - |
| HTP56_RS00315 | 49934..50752 | + | 819 | WP_032440576 | type IV secretory system conjugative DNA transfer family protein | traI |
| HTP56_RS00320 | 50730..51917 | + | 1188 | WP_077255878 | plasmid transfer ATPase TraJ | - |
| HTP56_RS00325 | 51914..52180 | + | 267 | WP_032440577 | IcmT/TraK family protein | traK |
| HTP56_RS00330 | 52198..55611 | + | 3414 | WP_052145516 | LPD7 domain-containing protein | - |
| HTP56_RS00335 | 55645..55998 | + | 354 | WP_032440584 | hypothetical protein | traL |
| HTP56_RS00340 | 56070..56738 | + | 669 | WP_032440585 | DotI/IcmL/TraM family protein | traM |
| HTP56_RS00345 | 56749..57852 | + | 1104 | WP_077264204 | DotH/IcmK family type IV secretion protein | traN |
| HTP56_RS00350 | 57856..59196 | + | 1341 | WP_032440586 | conjugal transfer protein TraO | traO |
| HTP56_RS00355 | 59203..59922 | + | 720 | WP_050597333 | conjugal transfer protein TraP | traP |
| HTP56_RS00360 | 59932..60465 | + | 534 | WP_032440587 | conjugal transfer protein TraQ | traQ |
| HTP56_RS00365 | 60521..60922 | + | 402 | WP_032440588 | DUF6750 family protein | traR |
| HTP56_RS00370 | 60966..61529 | + | 564 | WP_045325070 | hypothetical protein | traT |
| HTP56_RS00375 | 61539..64586 | + | 3048 | WP_032440589 | conjugal transfer protein | traU |
| HTP56_RS00380 | 64596..65792 | + | 1197 | WP_032440590 | conjugal transfer protein TraW | traW |
| HTP56_RS00385 | 65789..66370 | + | 582 | WP_050597335 | conjugal transfer protein TraX | - |
| HTP56_RS00390 | 66412..68556 | + | 2145 | WP_172692005 | DotA/TraY family protein | traY |
| HTP56_RS00395 | 68622..69245 | + | 624 | WP_032440592 | plasmid IncI1-type surface exclusion protein ExcA | - |
| HTP56_RS00400 | 69347..70765 | + | 1419 | WP_063666705 | hypothetical protein | trbA |
| HTP56_RS00405 | 70776..71867 | + | 1092 | WP_050597336 | thioredoxin fold domain-containing protein | trbB |
| HTP56_RS00410 | 71869..74061 | + | 2193 | WP_032440593 | F-type conjugative transfer protein TrbC | - |
| HTP56_RS00415 | 74107..74640 | + | 534 | WP_032440594 | phospholipase D family protein | - |
| HTP56_RS00420 | 74652..74903 | + | 252 | WP_113394424 | hypothetical protein | - |
| HTP56_RS00425 | 75478..76104 | + | 627 | WP_254913868 | RepA family replication protein | - |
Host bacterium
| ID | 7768 | GenBank | NZ_MG288684 |
| Plasmid name | pE20-qnrS | Incompatibility group | IncFIB |
| Plasmid size | 106790 bp | Coordinate of oriT [Strand] | 40744..40825 [+] |
| Host baterium | Klebsiella aerogenes strain E20 |
Cargo genes
| Drug resistance gene | qnrS1, aph(6)-Id, aph(3'')-Ib, aadA2 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |