Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 107330 |
| Name | oriT_pMRY13-134EAE_1  |
| Organism | Klebsiella aerogenes strain MRY13-134 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_AP018455 (64067..64172 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 30..35, 41..46 (CCGCCG..CGGCGG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 106 nt
>oriT_pMRY13-134EAE_1
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCATCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCATCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 4883 | GenBank | WP_004187323 |
| Name | Relaxase_HXF81_RS00470_pMRY13-134EAE_1 |
UniProt ID | A0A3U8KUL3 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75179.89 Da Isoelectric Point: 9.9948
>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3U8KUL3 |
T4CP
| ID | 5079 | GenBank | WP_176453628 |
| Name | t4cp2_HXF81_RS00200_pMRY13-134EAE_1 |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79521.84 Da Isoelectric Point: 4.8270
>WP_176453628.1 MULTISPECIES: conjugal transfer protein TrbC [Klebsiella]
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQETEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMHFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFADWLDGTDTDDVLYHQEDDLFPMIEM
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQETEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMHFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFADWLDGTDTDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 66968..86212
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HXF81_RS00450 | 62052..62363 | + | 312 | WP_011091065 | hypothetical protein | - |
| HXF81_RS00455 | 62496..63032 | + | 537 | WP_011091066 | hypothetical protein | - |
| HXF81_RS00460 | 63534..63929 | - | 396 | WP_176453640 | MobC | - |
| HXF81_RS00575 | 63964..64491 | + | 528 | WP_228260304 | plasmid mobilization protein MobA | - |
| HXF81_RS00470 | 64478..66457 | + | 1980 | WP_004187323 | TraI/MobA(P) family conjugative relaxase | - |
| HXF81_RS00475 | 66471..66971 | + | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| HXF81_RS00480 | 66968..67747 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| HXF81_RS00485 | 67758..68921 | + | 1164 | WP_129075152 | plasmid transfer ATPase TraJ | virB11 |
| HXF81_RS00490 | 68911..69171 | + | 261 | WP_129075151 | IcmT/TraK family protein | traK |
| HXF81_RS00580 | 69196..72588 | + | 3393 | WP_228715119 | LPD7 domain-containing protein | - |
| HXF81_RS00500 | 72512..73066 | + | 555 | WP_011154467 | hypothetical protein | traL |
| HXF81_RS00505 | 73067..73264 | - | 198 | WP_004187464 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| HXF81_RS00510 | 73251..73661 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| HXF81_RS00515 | 73660..74442 | + | 783 | WP_129075150 | DotI/IcmL family type IV secretion protein | traM |
| HXF81_RS00520 | 74451..75602 | + | 1152 | WP_129075149 | DotH/IcmK family type IV secretion protein | traN |
| HXF81_RS00525 | 75614..76963 | + | 1350 | WP_129075148 | conjugal transfer protein TraO | traO |
| HXF81_RS00530 | 76975..77679 | + | 705 | WP_129075147 | conjugal transfer protein TraP | traP |
| HXF81_RS00535 | 77703..78233 | + | 531 | WP_129075146 | conjugal transfer protein TraQ | traQ |
| HXF81_RS00540 | 78250..78639 | + | 390 | WP_020805690 | DUF6750 family protein | traR |
| HXF81_RS00545 | 78685..79179 | + | 495 | WP_228260310 | hypothetical protein | - |
| HXF81_RS00550 | 79176..82226 | + | 3051 | WP_129075145 | conjugal transfer protein | traU |
| HXF81_RS00555 | 82256..83431 | + | 1176 | WP_228715120 | conjugal transfer protein TraW | traW |
| HXF81_RS00560 | 83428..84024 | + | 597 | WP_129075144 | TraX-like protein | - |
| HXF81_RS00565 | 84017..86212 | + | 2196 | WP_129075143 | DotA/TraY family protein | traY |
| HXF81_RS00570 | 86214..86867 | + | 654 | WP_129075142 | hypothetical protein | - |
Host bacterium
| ID | 7766 | GenBank | NZ_AP018455 |
| Plasmid name | pMRY13-134EAE_1 | Incompatibility group | IncL/M |
| Plasmid size | 86881 bp | Coordinate of oriT [Strand] | 64067..64172 [+] |
| Host baterium | Klebsiella aerogenes strain MRY13-134 |
Cargo genes
| Drug resistance gene | blaIMP-1, ant(3'')-Ii-aac(6')-IId, qacE, sul1 |
| Virulence gene | - |
| Metal resistance gene | arsC, arsB, arsA, arsD, arsR, merE, merD, merA, merC, merP, merT, merR |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |