Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   107329
Name   oriT_pE20-NR in_silico
Organism   Klebsiella aerogenes strain E20
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MG288683 (73819..73868 [-], 50 nt)
oriT length   50 nt
IRs (inverted repeats)      7..14, 17..24  (GCAAAATT..AATTTTGC)
Location of nic site      33..34
Conserved sequence flanking the
  nic site    		  TGTGTGGTGA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 50 nt

>oriT_pE20-NR
AAATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTGATTTTGTGGTGAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   4882 GenBank   WP_029498346
Name   traI_HTP51_RS00650_pE20-NR insolico UniProt ID   A0A2R4NEL5
Length   1752 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1752 a.a.        Molecular weight: 190742.66 Da        Isoelectric Point: 6.1403

>WP_029498346.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacteriaceae]
MLSFSQVKSAGSAGNYYTEKDNYYVIGSMEERWQGKGAELLGLEGKVDKQVFTELLQGKLPDGSDLTRIQ
DGVNKHRPGYDLTFSAPKSVSMLAMLGGDKRLIDAHNRAVTVALNQVESLASTRVKKDGVSETVLTGNLI
IARFNHDTSRAQDPQIHTHSVVINATQNGDKWQTLASDTVGKTGFSETILANRIAFGKIYQNSLRADVES
MGYKTVDAGRNGMWEMEGVPVESFSTRSQELLEAAGPDASLKSRDVAALDTRKSKEAIDPAENMVEWMNT
LKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLEA
KDGVFDLARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVPF
SDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVTG
KSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTYR
WQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRHEGVLGEKDT
TITALTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTASSNMLTLKDRDGVRLDLKVSAVD
SQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAVGAGVFDGIK
IGHGWVESPGRSVSETAMVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSEQ
LKTRSGETDLDAAIAQQKAGLRTPAEQAIHLAIPLLESEKLTFSRPQLLATALETGGGKVPMADIDTTIQ
AQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKDAVAPLMDRVPASLMTDLTAGQRAATRM
ILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLHD
TQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQRS
AADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIEK
ALSEGKTLLEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITLP
VLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERYISPREASAEGVTLY
RQEKITVSQGDRMRFSKSDLERGYVANSIWEVQSVSGDSVTLSDGKLTRTLTPKADQAQQHIDLAYAITA
HGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDSREGWIKAIKHSPEKATAHDILEP
RNDRAVKSADLLFGRARPLDETAAGRAALQQSGLAQGNSPGKFISPGKKYPQPHVALPAFDKNGKAAGIW
LSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDRP
WNPGAITGGRIWAEPAPVAPVPQTGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEPA
DRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEEAIQQVASERLQRERLQAVERDMVRDLNREKTLG
GD

  Protein domains


Predicted by InterproScan.

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(634-713)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(576-628)

AAA domain (AAA_30)

(970-1156)

TrwC relaxase (TrwC)

(11-283)

DNA helicase TraI (TraI_C)

(1432-1554)


  Protein structure



No available structure.




T4CP

ID   5078 GenBank   WP_048255491
Name   traD_HTP51_RS00645_pE20-NR insolico UniProt ID   _
Length   760 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 760 a.a.        Molecular weight: 84914.98 Da        Isoelectric Point: 5.0560

>WP_048255491.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacteriaceae]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPEPGPADTDSHAGEQPEPVSPPA
PAEMTVSPAPVKAPPTTKRPAAEPPVLPVTPIPLLKPKAAAAAATASSAGTPAAAAGGTEQELAQQSAEQ
GQDMLPAGMNEDGVIEDMQAYAAWLADEQTQRDMQRREEVNINHNHRHDEQDDVEIGGNI

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(172-560)

F sex factor protein N terminal (TraD_N)

(32-128)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 73261..102472

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTP51_RS00415 68798..69154 + 357 WP_014343501 hypothetical protein -
HTP51_RS00420 69215..69427 + 213 WP_014343500 hypothetical protein -
HTP51_RS00425 69438..69662 + 225 WP_014343499 hypothetical protein -
HTP51_RS00430 69743..70063 + 321 WP_014343498 type II toxin-antitoxin system RelE/ParE family toxin -
HTP51_RS00435 70053..70331 + 279 WP_004152721 helix-turn-helix transcriptional regulator -
HTP51_RS00440 70335..70439 + 105 Protein_90 transcriptional regulator -
HTP51_RS00445 71268..71426 + 159 Protein_91 DUF945 domain-containing protein -
HTP51_RS00450 71482..72179 + 698 WP_095033700 IS1-like element IS1B family transposase -
HTP51_RS00455 72195..72866 + 672 Protein_93 DUF932 domain-containing protein -
HTP51_RS00460 72899..73228 + 330 WP_013214015 DUF5983 family protein -
HTP51_RS00465 73261..73746 - 486 WP_014343495 transglycosylase SLT domain-containing protein virB1
HTP51_RS00470 74137..74553 + 417 WP_014343494 conjugal transfer relaxosome DNA-binding protein TraM -
HTP51_RS00475 74753..75490 + 738 WP_013214018 conjugal transfer protein TrbJ -
HTP51_RS00480 75605..75811 + 207 WP_171773970 TraY domain-containing protein -
HTP51_RS00485 75873..76241 + 369 WP_004194426 type IV conjugative transfer system pilin TraA -
HTP51_RS00490 76255..76559 + 305 Protein_100 type IV conjugative transfer system protein TraL -
HTP51_RS00495 76579..77145 + 567 WP_016831035 type IV conjugative transfer system protein TraE traE
HTP51_RS00500 77132..77872 + 741 WP_047670045 type-F conjugative transfer system secretin TraK traK
HTP51_RS00505 77872..79296 + 1425 WP_015632498 F-type conjugal transfer pilus assembly protein TraB traB
HTP51_RS00510 79289..79885 + 597 WP_047670043 conjugal transfer pilus-stabilizing protein TraP -
HTP51_RS00515 79908..80492 + 585 WP_014386196 type IV conjugative transfer system lipoprotein TraV traV
HTP51_RS00520 80624..81034 + 411 WP_015632501 hypothetical protein -
HTP51_RS00525 81039..81308 + 270 WP_032433956 hypothetical protein -
HTP51_RS00530 81347..81565 + 219 WP_004171484 hypothetical protein -
HTP51_RS00535 81566..81877 + 312 WP_015632502 hypothetical protein -
HTP51_RS00540 81944..82348 + 405 WP_032433941 hypothetical protein -
HTP51_RS00545 82391..82780 + 390 WP_015632504 hypothetical protein -
HTP51_RS00550 82788..83186 + 399 WP_015632505 hypothetical protein -
HTP51_RS00555 83258..85897 + 2640 WP_015632506 type IV secretion system protein TraC virb4
HTP51_RS00560 85897..86286 + 390 WP_015632507 type-F conjugative transfer system protein TrbI -
HTP51_RS00565 86286..86921 + 636 WP_047670041 type-F conjugative transfer system protein TraW traW
HTP51_RS00570 86956..87357 + 402 WP_004194979 hypothetical protein -
HTP51_RS00575 87354..88343 + 990 WP_172691989 conjugal transfer pilus assembly protein TraU traU
HTP51_RS00580 88619..89266 + 648 WP_015632510 type-F conjugative transfer system pilin assembly protein TrbC trbC
HTP51_RS00585 89325..91280 + 1956 WP_015065533 type-F conjugative transfer system mating-pair stabilization protein TraN traN
HTP51_RS00590 91312..91566 + 255 WP_032433943 conjugal transfer protein TrbE -
HTP51_RS00595 91544..91792 + 249 WP_004152675 hypothetical protein -
HTP51_RS00600 91805..92131 + 327 WP_015632512 hypothetical protein -
HTP51_RS00605 92152..92904 + 753 WP_015632513 type-F conjugative transfer system pilin assembly protein TraF traF
HTP51_RS00610 92915..93154 + 240 WP_048255490 type-F conjugative transfer system pilin chaperone TraQ -
HTP51_RS00615 93126..93683 + 558 WP_023158002 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
HTP51_RS00620 93729..94172 + 444 WP_023158001 F-type conjugal transfer protein TrbF -
HTP51_RS00625 94150..95529 + 1380 WP_172691990 conjugal transfer pilus assembly protein TraH traH
HTP51_RS00630 95529..98378 + 2850 WP_023329044 conjugal transfer mating-pair stabilization protein TraG traG
HTP51_RS00635 98384..98911 + 528 WP_029498351 conjugal transfer protein TraS -
HTP51_RS00640 99101..99832 + 732 WP_004152629 conjugal transfer complement resistance protein TraT -
HTP51_RS00645 100190..102472 + 2283 WP_048255491 type IV conjugative transfer system coupling protein TraD virb4

Host bacterium

ID   7765 GenBank   NZ_MG288683
Plasmid name   pE20-NR Incompatibility group   IncFII
Plasmid size   112063 bp Coordinate of oriT [Strand]   73819..73868 [-]
Host baterium   Klebsiella aerogenes strain E20

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIE9