Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 107324 |
| Name | oriT_pLD-TEX-KL  |
| Organism | Fluoribacter dumoffii Tex-KL |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NC_009966 (28883..28915 [+], 33 nt) |
| oriT length | 33 nt |
| IRs (inverted repeats) | 3..9, 13..19 (ACGTTGC..GCAACGT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 33 nt
>oriT_pLD-TEX-KL
GCACGTTGCAACGCAACGTATAAGCGCGCACTT
GCACGTTGCAACGCAACGTATAAGCGCGCACTT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 5071 | GenBank | WP_012187529 |
| Name | traD_HXC02_RS00135_pLD-TEX-KL |
UniProt ID | _ |
| Length | 621 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 621 a.a. Molecular weight: 71124.44 Da Isoelectric Point: 7.1593
>WP_012187529.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Legionellaceae]
MNNEPNFKHYTRGGQISFHNLRMWDQITKTLSMICLIFWIVFTLVFVWFFISLEKITHAVIFYYAQFLEI
VGQKHTFELSFHGKTYKQTVDSILNYAYYQDNANHVIHSLGKSALWAFCLSFILGLCLAYYFIKRGKAQS
DSQFVRGSQFKHSNVVKKKILRDKAHSDITIDTFPLIKNSEVQHVLVHGTVGTGKSQLIMKIMDCLRKRG
DRVIVYDKGCSFIAHYYQDESDVILNPFDKRCANWDMWLEAPRDSDFENMAESSIPMHGESDPFWVNASR
TVFSCLGSVMRHHSDRSVEKLLKLILTDEFSDLEEYLQGTPAATLVSSKIEKTAISIRATLTTYLKSFSA
LAELNQEGKPPFSIRDYILDEQQKGWLFISSNGETHKSLKPLISMWLAQASLALLSLTPDRNRRIWFICD
ELPSLHKLPLLGETIAEVRKFGGCFLLGMQSFSQLTKVYGQAGGREIFDLLNTRFFFRSPSSDMARLVAS
ELGEEEIEESRENYSYGANSIRDGISLGAQRVTRPIVSYPQIMELKDLHCFVRLPGHYPITQLTLDLGFR
TIKTNGFIERNMPTTFNFSQLTSIDEDWESGTDGHVGSKQPNKNKINAEHQCIEELNLEPL
MNNEPNFKHYTRGGQISFHNLRMWDQITKTLSMICLIFWIVFTLVFVWFFISLEKITHAVIFYYAQFLEI
VGQKHTFELSFHGKTYKQTVDSILNYAYYQDNANHVIHSLGKSALWAFCLSFILGLCLAYYFIKRGKAQS
DSQFVRGSQFKHSNVVKKKILRDKAHSDITIDTFPLIKNSEVQHVLVHGTVGTGKSQLIMKIMDCLRKRG
DRVIVYDKGCSFIAHYYQDESDVILNPFDKRCANWDMWLEAPRDSDFENMAESSIPMHGESDPFWVNASR
TVFSCLGSVMRHHSDRSVEKLLKLILTDEFSDLEEYLQGTPAATLVSSKIEKTAISIRATLTTYLKSFSA
LAELNQEGKPPFSIRDYILDEQQKGWLFISSNGETHKSLKPLISMWLAQASLALLSLTPDRNRRIWFICD
ELPSLHKLPLLGETIAEVRKFGGCFLLGMQSFSQLTKVYGQAGGREIFDLLNTRFFFRSPSSDMARLVAS
ELGEEEIEESRENYSYGANSIRDGISLGAQRVTRPIVSYPQIMELKDLHCFVRLPGHYPITQLTLDLGFR
TIKTNGFIERNMPTTFNFSQLTSIDEDWESGTDGHVGSKQPNKNKINAEHQCIEELNLEPL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 7435..25432
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HXC02_RS00020 | 2793..3326 | + | 534 | WP_058460589 | hypothetical protein | - |
| HXC02_RS00025 (pLDTEXKL_p05) | 3295..4068 | - | 774 | WP_058460590 | VUT family protein | - |
| HXC02_RS00030 (pLDTEXKL_p06) | 4052..4711 | - | 660 | WP_012187509 | XRE family transcriptional regulator | - |
| HXC02_RS00335 | 4911..5153 | + | 243 | WP_012187510 | TraY domain-containing protein | - |
| HXC02_RS00040 (pLDTEXKL_p08) | 5131..6222 | + | 1092 | WP_012187511 | hypothetical protein | - |
| HXC02_RS00045 (pLDTEXKL_p09) | 6273..7016 | + | 744 | WP_012187512 | complement resistance protein TraT | - |
| HXC02_RS00050 (pLDTEXKL_p10) | 7134..7421 | + | 288 | WP_012187513 | hypothetical protein | - |
| HXC02_RS00055 (pLDTEXKL_p11) | 7435..7731 | + | 297 | WP_012187514 | type IV conjugative transfer system protein TraL | traL |
| HXC02_RS00060 (pLDTEXKL_p12) | 7721..8311 | + | 591 | WP_236942459 | type IV conjugative transfer system protein TraE | traE |
| HXC02_RS00065 (pLDTEXKL_p13) | 8304..9023 | + | 720 | WP_012187516 | type-F conjugative transfer system secretin TraK | traK |
| HXC02_RS00070 (pLDTEXKL_p14) | 9016..10389 | + | 1374 | WP_012187517 | TrbI/VirB10 family protein | traB |
| HXC02_RS00075 | 10449..10676 | + | 228 | WP_231294685 | conjugal transfer protein | traV |
| HXC02_RS00080 (pLDTEXKL_p15) | 10667..13264 | + | 2598 | WP_012187518 | type IV secretion system protein TraC | virb4 |
| HXC02_RS00085 (pLDTEXKL_p16) | 13255..13590 | + | 336 | WP_012187519 | type-F conjugative transfer system protein TrbI | - |
| HXC02_RS00090 (pLDTEXKL_p17) | 13587..14222 | + | 636 | WP_012187520 | type-F conjugative transfer system protein TraW | traW |
| HXC02_RS00095 (pLDTEXKL_p18) | 14212..15195 | + | 984 | WP_058460577 | conjugal transfer pilus assembly protein TraU | traU |
| HXC02_RS00100 (pLDTEXKL_p19) | 15208..15630 | + | 423 | WP_012187522 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| HXC02_RS00105 (pLDTEXKL_p20) | 15623..17311 | + | 1689 | WP_012187523 | conjugal transfer protein TraN | traN |
| HXC02_RS00110 (pLDTEXKL_p21) | 17304..18053 | + | 750 | WP_012187524 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| HXC02_RS00115 (pLDTEXKL_p22) | 18050..18559 | + | 510 | WP_012187525 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
| HXC02_RS00120 (pLDTEXKL_p23) | 18559..19923 | + | 1365 | WP_012187526 | conjugal transfer protein TraH | traH |
| HXC02_RS00125 (pLDTEXKL_p24) | 19937..22786 | + | 2850 | WP_012187527 | conjugal transfer mating-pair stabilization protein TraG | traG |
| HXC02_RS00130 (pLDTEXKL_p25) | 22776..23567 | + | 792 | WP_058387442 | hypothetical protein | - |
| HXC02_RS00135 (pLDTEXKL_p26) | 23567..25432 | + | 1866 | WP_012187529 | type IV conjugative transfer system coupling protein TraD | virb4 |
| HXC02_RS00140 (pLDTEXKL_p27) | 25517..26134 | - | 618 | WP_012187530 | hypothetical protein | - |
| HXC02_RS00145 (pLDTEXKL_p28) | 26145..28952 | - | 2808 | WP_236942460 | Ti-type conjugative transfer relaxase TraA | - |
| HXC02_RS00150 (pLDTEXKL_p29) | 29042..29332 | + | 291 | WP_012187532 | hypothetical protein | - |
| HXC02_RS00155 (pLDTEXKL_p30) | 29549..29857 | + | 309 | WP_012187533 | hypothetical protein | - |
Host bacterium
| ID | 7760 | GenBank | NC_009966 |
| Plasmid name | pLD-TEX-KL | Incompatibility group | - |
| Plasmid size | 66512 bp | Coordinate of oriT [Strand] | 28883..28915 [+] |
| Host baterium | Fluoribacter dumoffii Tex-KL |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA1, AcrIB |