Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   107320
Name   oriT_pNDM1_EC14653 in_silico
Organism   Enterobacter cloacae strain WCHECl-14653
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_KP868647 (57466..57568 [+], 103 nt)
oriT length   103 nt
IRs (inverted repeats)      89..94, 96..101  (AAATAA..TTATTT)
 28..33, 40..45  (GCAAAA..TTTTGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 103 nt

>oriT_pNDM1_EC14653
CTCACCACCAAAAGTTCCACACCCCACGCAAAATTAAAGTTTTGCTGATTGGGTATTCAAATCATGCAGTTAGTGAAAAAGTAATGTGAAATAATTTATTTTA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   4875 GenBank   WP_016479978
Name   traI_HTF20_RS00460_pNDM1_EC14653 insolico UniProt ID   A0A0G3B767
Length   1746 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1746 a.a.        Molecular weight: 189285.41 Da        Isoelectric Point: 6.0099

>WP_016479978.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacterales]
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMGERWAGKGAEALGLSGGVDQKVFTKVLEGRLPDGSDLSRTQ
DGANKHRPGYDFTFSAPKSVSVMAMLGDDKRLIEAHNRAVETAIKQIEAMASTRVMTEGRSETQLTGNLV
MALFNHDTSRDQEPQLHTHAVVANATQHGDEWRTLSSDKVGKTGFIENIYANQIAFGRLYRAALKDDVAA
MGYETETVGKHGMWELKGVPTEPYSSRSRTISEAVGDDASLKSRDVAALDTRQSKQKVDPEQRMAEWMQT
LKETGFDIKAYREAADLRVVQGNIPATTPEAIDINSSVGQAIAMLSDRRARFTYSELLATTLGQLPARSG
MVEMARDGIDAAIKNEQLIPLDKEKGLFTSNIHVLDELSVSAMTRDLQRTGQADIFPDKSVPRSRSYSDA
VSVLAQDRPPVAIISGQGGAAGQRERVAELTMMAREQGRDVQIIAADRRSRSALMQDERLSGEHITDRRG
LTEGMAFTPGSTLIVDQGEKLTLKETLTLLDGALRHNVQLLIADSGQRTGTGSALTVMKEAGVSTLAWQG
GEKTQVSVISEPDRRQRYDRLAADFARSVRAGEHSVAQVTGPREQAVLAGVVREALKAEKVLGEREVSIT
TLEPVWLDSRHQQVRDHYREGMVMERWNAEERSRERFVIDRVTGRNNSLTLRNAQGETRVTRLSELNSSW
SLYRTGTLQVAEGDRLAVLGQAQGTRLRGGDTVTVKALGEAGLVVSLPGRKADVVLPAGDSPFTAAKVVQ
GWVESPGRSVSDSATVFASLSQRELDNTTLNKLALSGRAVRLYSAQTAQKTTEKLSRQSAWSVVSEQIKD
AAGHDRLDDALAHQKAGLHTPEQQAIHLAIPVLEGNGLAFTKPQLMAAAKEFEQDSLSLQAIEKEADRQV
RSGDLLSVPVSPGNGLQLLVSRQSYNAEKSIIRHVLEGKEAVTPLMDKVPDAQLAGLTDGQRNATRMILE
SPDRFTLVQGYAGVGKTTQFRAVMSAIGTLPEREQPRVIGVAPTHRAVSEMRDAGVPETQTLAAFIHDTQ
QQLRGGERTDFSNVLFLVDESSMVGNADMAKAYSLIAGGGGRAVSSGDTDQLQSIAPGQPFRLLQKRSAI
DVAVMQEIVRQTPELKPAVYSLISRDIGSALTTIESVAPAQVPRRADAWQPDSSVMEFSREREEAIAKAV
AAGDLMPGGQPATLLEAIVKDYTGRTSEAQAQTIVITALNADRRQVNAMIHDARQGAGEVGEKEVTLPVL
TPANIRDGELRRMATWEASRDSLVLLDSTYYSIEALDGEAHLVMLKDAQGNTRSLSPAQAATEGVTLYRQ
DTITVSEGDRMRFSKSDNERGFVANSVWSVSEIKGDSVTLSDGKQTRTLTPSAEQAEQHIDLAYAVTVEG
SQGASEPFSISLQGTDGGRKRMVSFESAYVALSRMKQHAQVYTDNRDKWAAAMEKSQAKSTAHDILEPRG
DRAVANAARLTATAKALGEVPAGRAALRQAGLQPEGSMAKYISPGRKYPQPHVALPAFDRNGRKAGVWLS
ALTSGDGQLKGLAGEGRVMGSGDAAFAGLQASRNGESLLARDMEEGVRLARENPQSGVVVRLEGEARPWN
PGAITGGRVWADGLPDATGTQPAETVPPEVLAQQAREAEVQRELDKRAEEAVREMARSGDRPAGGADVAV
RDVVRDMDRIKETPASPLTLPDAPEERRRDEAVSQVVRESVQRDRLQQMERETVRDLEREKTLGGD

  Protein domains


Predicted by InterproScan.

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(631-710)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(573-625)

TrwC relaxase (TrwC)

(10-283)

DNA helicase TraI (TraI_C)

(1430-1551)

AAA domain (AAA_30)

(967-1150)


  Protein structure



No available structure.




T4CP

ID   5067 GenBank   WP_008324134
Name   traC_HTF20_RS00380_pNDM1_EC14653 insolico UniProt ID   _
Length   876 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 876 a.a.        Molecular weight: 98841.51 Da        Isoelectric Point: 5.7676

>WP_008324134.1 MULTISPECIES: type IV secretion system protein TraC [Enterobacterales]
MSNPLDSVTRTVNSLLTALKMPDASSEANDVLGQMRFPQFSRVLPYRDYDAETGLFMNEKTMGFMLEAQP
INGANETIVSSLENLMRTKLPREVPVAVHLVSSHLVGQDIEYGLRELSWTGEKADTFNAITRAYYLRAAE
EGFPLPDHLNLPLTLRNYRVYISCCVPRKRGSKASVMEMENQVKIIRASLQGAQIATRSVDADAFIRIAG
EMINHNPDALYPTERELDPLKDLNYQCVDDTFDLKVKADYMTLGLRGNGRNSTARIMNFQLTKNPELAFL
WNMADNYSNLLNPELSVSCPFILTLVLSVEDQVKAQNEANLKFMDLEKKSKTSYAKFFPGVAQEAKEWGD
LRQRLSSGQSSLVSYFLNVTLFCRDDQETALKVEQDVLNSFRKNGFELISPRFNHMRNFLVNLPFMAGGG
LFKQLRESGVLQRAESFNVANLMPVVADSPLATSGLLAPTYRNQLAFIDLFSRGMGNTNFNMAVCGTSGA
GKTGLIQPLIRSVIDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLKFNPFANVTDDTIDEMAERLRDQL
SVMASPNGNLDEVHEGLLLKAVKATWLTKKNQARIDDVVDYLMMARDSEEYSGSPTIRSRLDEMVVLLTQ
YTRDGVYGSYFNSDEPSLKDEARMVVLELGGLEDRPSLLVAVMFSLILYIENRMYRTPRSLKKLNVIDEG
WRLLDFKNNKVGAFIEKGYRTARRHTGSYITITQNIVDFDSETASSAARAAWGNSSYKIILKQSAKEFAK
YNQLYPDQYSQLQRDMIKGFGSAKDNWFSSFMLSVEHLTSWHRLFVDPLSRAMYSSDGPDFEFLQQRRRE
GLSIHEAVWELAWKKSGPEMAALERWLHEHEGKTIS

  Protein domains


Predicted by InterproScan.

Family of unknown function (DUF5934) (DUF5934)

(289-446)

TraG P-loop domain (P-loop_TraG)

(470-765)

TraC protein (TraC_F_IV)

(38-276)

  Protein structure



No available structure.



ID   5068 GenBank   WP_016479979
Name   traD_HTF20_RS00455_pNDM1_EC14653 insolico UniProt ID   _
Length   748 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 748 a.a.        Molecular weight: 83951.64 Da        Isoelectric Point: 5.1609

>WP_016479979.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacterales]
MSFNAKDMTQGGQIASMRIRMFSQIANIIVYCLVILFGIIVAITLSVKLTWQTFVNGMVYYWCQTLAPMR
DIFKSQPVYTIRYYGHELKYNAQQILQDKYIQWCADQVWTAFILAAIIAAVVCVITFFVTTWILGHQGKQ
QSEDEITGGRQLTDDPKAVAKMLRRDGLASDIKIGELPLIKDSEIQNFCLHGTVGAGKSEVIRRLMNYAR
QRGDMVIVYDRSCEFVKSYYDPATDKILNPLDARCAAWDLWRECLTQPDFDNVSNTLIPMGTKEDPFWQG
SGRTIFAEAAYLMRNDKDRSYSKLVDTLLSIKIEKLRSYLKNSPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIENNGESFTIRDWMRGVREDGKNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVIGIQSYAQLEDIYGQKPAETLFDVLNTRAFFRSPSRNIA
EFAAGEIGEKEILKASEQYSYGVDPVRDGTSVGKDMERVTLVSYSDVQTLPDLSCYVTLPGPYPAVKMAL
KYQQRPKVAPEFIPRTIDGKAEERLNAALAAREHESRSIAMLFEPDADVVAPEGGAENAGQPQQPQQPQQ
PQQPQQPQQPQQPQQPQQPQQPQQPQQLTPAPRAADAASVAGAAGAGGVEPELKTKAEEAEQLPPGISES
GEVVDMAAWEAWQAEQDELSPQERQRREEVNINVHRAPEKDLEPGGYI

  Protein domains


Predicted by InterproScan.

F sex factor protein N terminal (TraD_N)

(32-128)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(172-560)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 56918..82089

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTF20_RS00270 (pNDM1_EC14653_00058) 52015..52338 + 324 WP_008324183 hypothetical protein -
HTF20_RS00275 (pNDM1_EC14653_00059) 52400..52759 + 360 WP_015493069 hypothetical protein -
HTF20_RS00280 (pNDM1_EC14653_00060) 53386..53760 + 375 WP_008324180 hypothetical protein -
HTF20_RS00285 (pNDM1_EC14653_00061) 53814..54101 + 288 WP_008324178 hypothetical protein -
HTF20_RS00290 (pNDM1_EC14653_00062) 54148..54492 + 345 WP_008324177 hypothetical protein -
HTF20_RS00295 (pNDM1_EC14653_00063) 54578..54841 + 264 WP_008324174 hypothetical protein -
HTF20_RS00300 (pNDM1_EC14653_00064) 54884..55714 + 831 WP_008324171 N-6 DNA methylase -
HTF20_RS00305 (pNDM1_EC14653_00065) 56341..56871 + 531 WP_008324170 antirestriction protein -
HTF20_RS00310 (pNDM1_EC14653_00066) 56918..57394 - 477 WP_008324168 transglycosylase SLT domain-containing protein -
HTF20_RS00315 (pNDM1_EC14653_00067) 57826..58218 + 393 WP_008324167 conjugal transfer relaxosome DNA-binding protein TraM -
HTF20_RS00320 (pNDM1_EC14653_00068) 58407..59096 + 690 WP_008324166 hypothetical protein -
HTF20_RS00325 (pNDM1_EC14653_00069) 59183..59407 + 225 WP_008324165 TraY domain-containing protein -
HTF20_RS00330 (pNDM1_EC14653_00070) 59465..59815 + 351 WP_016247536 type IV conjugative transfer system pilin TraA -
HTF20_RS00335 (pNDM1_EC14653_00071) 59966..60271 + 306 WP_016479987 type IV conjugative transfer system protein TraL traL
HTF20_RS00340 (pNDM1_EC14653_00072) 60286..60852 + 567 WP_016247535 type IV conjugative transfer system protein TraE traE
HTF20_RS00345 (pNDM1_EC14653_00073) 60839..61582 + 744 WP_008324157 type-F conjugative transfer system secretin TraK traK
HTF20_RS00350 (pNDM1_EC14653_00074) 61569..62963 + 1395 WP_016479986 F-type conjugal transfer pilus assembly protein TraB traB
HTF20_RS00355 (pNDM1_EC14653_00075) 62985..63524 + 540 WP_016247533 type IV conjugative transfer system lipoprotein TraV traV
HTF20_RS00360 (pNDM1_EC14653_00076) 63644..63868 + 225 WP_008324141 TraR/DksA C4-type zinc finger protein -
HTF20_RS00365 (pNDM1_EC14653_00077) 63872..64057 + 186 WP_008324139 hypothetical protein -
HTF20_RS00370 (pNDM1_EC14653_00078) 64041..64442 + 402 WP_016479985 hypothetical protein -
HTF20_RS00375 (pNDM1_EC14653_00079) 64432..64998 + 567 WP_008324135 conjugal transfer pilus-stabilizing protein TraP -
HTF20_RS00380 (pNDM1_EC14653_00080) 64991..67621 + 2631 WP_008324134 type IV secretion system protein TraC virb4
HTF20_RS00385 (pNDM1_EC14653_00081) 67618..67965 + 348 WP_008324133 type-F conjugative transfer system protein TrbI -
HTF20_RS00390 (pNDM1_EC14653_00082) 67965..68612 + 648 WP_008324132 type-F conjugative transfer system protein TraW traW
HTF20_RS00395 (pNDM1_EC14653_00083) 68615..69601 + 987 WP_042934586 conjugal transfer pilus assembly protein TraU traU
HTF20_RS00400 (pNDM1_EC14653_00084) 69618..70247 + 630 WP_008324130 type-F conjugative transfer system pilin assembly protein TrbC trbC
HTF20_RS00405 (pNDM1_EC14653_00085) 70244..72097 + 1854 WP_016479983 type-F conjugative transfer system mating-pair stabilization protein TraN traN
HTF20_RS00410 (pNDM1_EC14653_00086) 72129..72317 + 189 WP_008324128 conjugal transfer protein TrbE -
HTF20_RS00415 (pNDM1_EC14653_00087) 72348..73097 + 750 WP_008324127 type-F conjugative transfer system pilin assembly protein TraF traF
HTF20_RS00420 (pNDM1_EC14653_00088) 73114..73362 + 249 WP_016479982 type-F conjugative transfer system pilin chaperone TraQ -
HTF20_RS00425 (pNDM1_EC14653_00089) 73352..73906 + 555 WP_008324125 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
HTF20_RS00430 (pNDM1_EC14653_00090) 73899..74327 + 429 WP_008324122 hypothetical protein -
HTF20_RS00435 (pNDM1_EC14653_00091) 74314..75684 + 1371 WP_016247527 conjugal transfer pilus assembly protein TraH traH
HTF20_RS00440 (pNDM1_EC14653_00092) 75684..78506 + 2823 WP_008324119 conjugal transfer mating-pair stabilization protein TraG traG
HTF20_RS00445 (pNDM1_EC14653_00093) 78528..78959 + 432 WP_008324118 entry exclusion protein -
HTF20_RS00615 (pNDM1_EC14653_00094) 79034..79372 + 339 WP_032072912 tetratricopeptide repeat protein -
HTF20_RS00620 (pNDM1_EC14653_00095) 79380..79709 + 330 WP_223856629 hypothetical protein -
HTF20_RS00455 (pNDM1_EC14653_00096) 79843..82089 + 2247 WP_016479979 type IV conjugative transfer system coupling protein TraD virb4

Host bacterium

ID   7756 GenBank   NZ_KP868647
Plasmid name   pNDM1_EC14653 Incompatibility group   IncFIB
Plasmid size   109353 bp Coordinate of oriT [Strand]   57466..57568 [+]
Host baterium   Enterobacter cloacae strain WCHECl-14653

Cargo genes

Drug resistance gene   blaNDM-1
Virulence gene   htpB
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -