Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   107217
Name   oriT_pN7435-R3645 in_silico
Organism   Enterococcus faecium strain N7435
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MT022086 (72822..73013 [-], 192 nt)
oriT length   192 nt
IRs (inverted repeats)      106..112, 122..128  (ATTTTTT..AAAAAAT)
 107..113, 120..126  (TTTTTTG..CAAAAAA)
 93..98, 105..110  (AAAATA..TATTTT)
 65..71, 75..81  (AGTTGGC..GCCAACT)
 41..49, 52..60  (CACCTTCCT..AGGAAGGTG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 192 nt

>oriT_pN7435-R3645
CCAAGGAATTAATACAAAGAGCATAAGGGAAAACTGATAGCACCTTCCTAAAGGAAGGTGGCTAAGTTGGCTGTGCCAACTGGTTTTCTTTCAAAATAGCTTCATATTTTTTGCTATCACAAAAAAATCCATTTTCGACCTATTTTCGGTCATAATATAGTACCTACTTTTGGTCATAGTTTCGTCCGTAGT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   4798 GenBank   WP_002311868
Name   mobP2_JSX49_RS00430_pN7435-R3645 insolico UniProt ID   _
Length   511 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 511 a.a.        Molecular weight: 61114.57 Da        Isoelectric Point: 9.1583

>WP_002311868.1 MobP2 family relaxase [Enterococcus faecium]
MVGDDMTEHIFNRKSASIILKAKFETGKSKKNNHMVELQKFVDYISRQEAIRQDKLDHDYSEDELNELAR
IEKALEKIEQETDQPVIKDLREMDKYIDYMTRKKAILENVDNEIVNGAFSNTKRYITKKDISKIKESVIE
AKNNGSVMFQDVISFDNDFLVREGYYNPETNELNENVLYEATKSMMGKMQEKEELVDPFWFATIHRNTEH
IHIHVTAMERKNTREIMEYDGVLQARGKRKQSTLDDMIFKFGSKILDRTNEFEKISKLRKEVPLELKQSV
KDSLIQLYVENNSRYDKELVKYLKELKKEIPSTTRGYNELPEETKEKIDEVTNYMTKDNPKRKEYDRMTK
EIDELYQTTYGKRYNPQEYYLNRKKDFNARMGNAVVGQIKLLKQSEEKNLKSNELKNLLDGKKLNVKFDR
EKPEFKTKYAKQSYDDFQKRLEERQLAREKNEVHFKKISEKFKDKKLIRKIDRVINDDLNMYRAKNDYEV
AQRMIEQEKMRQAREFEIGMN

  Protein domains


Predicted by InterproScan.

MobL relaxases (MobL)

(16-443)


  Protein structure



No available structure.




T4CP

ID   4965 GenBank   WP_202979417
Name   t4cp2_JSX49_RS00360_pN7435-R3645 insolico UniProt ID   _
Length   897 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 897 a.a.        Molecular weight: 102099.96 Da        Isoelectric Point: 6.4133

>WP_202979417.1 VirD4-like conjugal transfer protein, CD1115 family [Enterococcus faecium]
MNVPLVFIGVFLGSIVFLAITYLLNFLKALLVAIGQSESGLLGVEFDPKFSLAYLKPLGDTKQYFMCAVV
AVIGVAYLIYGKLNHESEKNLVYGQKGDSRLSTIKEIKRDYHEIPEKTEVYDGIGGVPISHYQDKYYIDR
DTVNTVVLGASRSGKGETFVVPLLDNLSRAKIQSNMVVNDPKGELFSASKETLEKRGYRVLVLNIDDPLE
SMSFNPLQLVIDSWANKDYHEASKRANTLTSMLFASGMGTDNEFFYKSAKSAVNAIILTIVEHCFNNDCI
EKITMYNVAQMLNELGSLFYTDPKTRKEKNALDEYFNTLPQGNQAKIQYGSTSFAGDKAKGSILSTASQG
IEMFTSDLFGKLTSKMSIDLKEIGFPKSIQFKVNTNLVGKRISISFLRKENDVIRLIKKYRVKVKALGLC
VLNFNEYLQDGDMLDIRYEEEKSRAWYRIEFPKKQANSHDTSLVTKKFKSGKNYSDLEIITLRLKYTDQP
TAVFMVIPDYDPSNHVLASIFISQLYTELASNCKQTPDKKCFRRVHFLLDEFGNMPAIDNMDGIMTVCLG
RNMLFDLVIQSYSQLETRYDKAFKTIKENCQNHILIMSNDEETIEEISKKCGHKTTINRSASSKHLDTDS
SVTSSAEQERVITVERASQLIEGEQIILRNLHRQDTKRNKIRPFPIFNTKETNMPYRYQFLAEDFDTQRD
INEIDIACEHINLSLQDNQIPYARFIKDLKTRLEYSIINNIPISEEDYQDYLNLSGSQQTINEEELLKLV
NTEVKSQDMEGQVLSEQEMKINIYLKESQKEAIELVKVINGRLADENSADMVIKTINSQILDTPFIQSLI
NDSIAIHNVQESKDQIMILEQQLANAYELAVFKTKNSILNNKIIKLKNAMTKLAKAI

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(534-646)

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(144-387)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 52074..81493

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSX49_RS00290 47355..50984 + 3630 WP_002311844 SpaA isopeptide-forming pilin-related protein -
JSX49_RS00295 51044..51406 + 363 WP_002311845 hypothetical protein -
JSX49_RS00300 51420..51572 + 153 WP_002311846 hypothetical protein -
JSX49_RS00305 51640..52062 + 423 WP_002311847 hypothetical protein -
JSX49_RS00310 52074..53489 + 1416 WP_002311848 CpaF/VirB11 family protein virB11
JSX49_RS00315 53506..54321 + 816 WP_002313315 hypothetical protein -
JSX49_RS00320 54321..55118 + 798 WP_002311850 hypothetical protein -
JSX49_RS00325 55136..55498 + 363 WP_002311851 DUF4320 family protein -
JSX49_RS00330 55512..55658 + 147 WP_002326866 hypothetical protein -
JSX49_RS00335 55670..55891 + 222 WP_002326867 hypothetical protein -
JSX49_RS00340 55910..56185 + 276 WP_002311852 TrbC/VirB2 family protein virB2
JSX49_RS00345 56294..58120 + 1827 WP_002311853 hypothetical protein -
JSX49_RS00350 58133..58849 + 717 WP_202979415 hypothetical protein -
JSX49_RS00355 58862..59029 + 168 WP_202979416 hypothetical protein -
JSX49_RS00360 59026..61719 + 2694 WP_202979417 VirD4-like conjugal transfer protein, CD1115 family -
JSX49_RS00365 61770..64420 + 2651 Protein_72 pLS20_p028 family conjugation system transmembrane protein -
JSX49_RS00370 64434..64778 + 345 WP_202979418 DUF5592 family protein -
JSX49_RS00375 64775..65392 + 618 WP_002311858 hypothetical protein virb4
JSX49_RS00550 65409..65645 + 237 WP_238939378 hypothetical protein -
JSX49_RS00385 66068..67516 + 1449 WP_238939379 tra protein virb4
JSX49_RS00390 68204..69244 + 1041 WP_202979419 HNH endonuclease -
JSX49_RS00395 69432..69752 + 321 WP_010729568 hypothetical protein virb4
JSX49_RS00400 69774..70910 + 1137 WP_002311861 bifunctional lytic transglycosylase/C40 family peptidase orf14
JSX49_RS00405 70925..71575 + 651 WP_002311862 cell division protein FtsL -
JSX49_RS00410 71589..72485 + 897 WP_202979420 hypothetical protein -
JSX49_RS00415 72521..72751 + 231 WP_002311864 hypothetical protein -
JSX49_RS00420 73102..73398 + 297 WP_002311865 hypothetical protein -
JSX49_RS00425 73395..73862 + 468 WP_002311867 hypothetical protein -
JSX49_RS00430 73929..75464 + 1536 WP_002311868 MobP2 family relaxase -
JSX49_RS00435 75486..75815 + 330 WP_002311869 hypothetical protein -
JSX49_RS00440 75817..76080 + 264 WP_002311870 hypothetical protein -
JSX49_RS00445 76255..77121 + 867 WP_123061272 hypothetical protein -
JSX49_RS00450 77276..79102 + 1827 WP_123061273 ArdC-like ssDNA-binding domain-containing protein -
JSX49_RS00455 79110..80183 + 1074 WP_002311873 hypothetical protein -
JSX49_RS00460 80192..80452 + 261 WP_002311874 hypothetical protein -
JSX49_RS00465 80489..81493 + 1005 WP_002311875 DUF3991 domain-containing protein traP
JSX49_RS00470 81518..81874 + 357 WP_002311876 hypothetical protein -
JSX49_RS00475 82336..82554 + 219 WP_123061274 hypothetical protein -
JSX49_RS00480 82591..82908 + 318 WP_011377381 hypothetical protein -
JSX49_RS00485 82919..83116 + 198 WP_123061275 hypothetical protein -
JSX49_RS00490 83113..84081 + 969 WP_123061276 zeta toxin family protein -
JSX49_RS00495 84325..85497 + 1173 WP_123061286 hypothetical protein -

Host bacterium

ID   7653 GenBank   NZ_MT022086
Plasmid name   pN7435-R3645 Incompatibility group   -
Plasmid size   92396 bp Coordinate of oriT [Strand]   72822..73013 [-]
Host baterium   Enterococcus faecium strain N7435

Cargo genes

Drug resistance gene   lsa(E), erm(B), ant(6)-Ia
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA1