Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 107124 |
| Name | oriT_pEAR5.2  |
| Organism | Erwinia amylovora ATCC BAA-2158 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NC_018985 (2955..3014 [+], 60 nt) |
| oriT length | 60 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 60 nt
>oriT_pEAR5.2
GGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCTTA
GGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCTTA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 4722 | GenBank | WP_176703083 |
| Name | Relaxase_G7C09_RS00025_pEAR5.2 |
UniProt ID | _ |
| Length | 269 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 269 a.a. Molecular weight: 29851.75 Da Isoelectric Point: 8.0106
>WP_176703083.1 relaxase/mobilization nuclease domain-containing protein [Erwinia amylovora]
MLPMIVKFHPRGRGGGAGPVDYLLGKDRQRDGASLLQGKPEEVRELIDASPYAKKYTTGVLSFAENDLPP
GQREKLMASFERVLMPGLDKDQYSVLWVEHRDKGRLELNFLIPNTELLTGRRLQPYYDRADRPRIDAWQT
IVNGRLGLHDPNAPENRRALVTPSALPEAKQEAAQAITRGLLALASSGELKTRQDVTEALESAGFEVVRT
TKSSISIADPDGGRNIRLKGAIYEQSFNAGEGLRAEIESAAADYRRDAESRIQRAREVC
MLPMIVKFHPRGRGGGAGPVDYLLGKDRQRDGASLLQGKPEEVRELIDASPYAKKYTTGVLSFAENDLPP
GQREKLMASFERVLMPGLDKDQYSVLWVEHRDKGRLELNFLIPNTELLTGRRLQPYYDRADRPRIDAWQT
IVNGRLGLHDPNAPENRRALVTPSALPEAKQEAAQAITRGLLALASSGELKTRQDVTEALESAGFEVVRT
TKSSISIADPDGGRNIRLKGAIYEQSFNAGEGLRAEIESAAADYRRDAESRIQRAREVC
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Auxiliary protein
| ID | 2135 | GenBank | WP_176703082 |
| Name | WP_176703082_pEAR5.2 |
UniProt ID | _ |
| Length | 165 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 165 a.a. Molecular weight: 18459.26 Da Isoelectric Point: 10.2296
>WP_176703082.1 MbeB family mobilization protein [Erwinia amylovora]
MSSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVKAELNESEKRISAAILDHDRKLSSVMSQRTKGM
VRMVSQTWLTIVLVSVLLIASSAGILWWQGNQMLDNYMSIREQKDALEKLNAKTFGVSFQSDSNGQFLVL
PKGVKAETSWTVENGKRNAVKLVRE
MSSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVKAELNESEKRISAAILDHDRKLSSVMSQRTKGM
VRMVSQTWLTIVLVSVLLIASSAGILWWQGNQMLDNYMSIREQKDALEKLNAKTFGVSFQSDSNGQFLVL
PKGVKAETSWTVENGKRNAVKLVRE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 2136 | GenBank | WP_176703084 |
| Name | WP_176703084_pEAR5.2 |
UniProt ID | _ |
| Length | 107 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 107 a.a. Molecular weight: 11762.60 Da Isoelectric Point: 7.2165
>WP_176703084.1 MobC family plasmid mobilization relaxosome protein [Erwinia amylovora]
MLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPAKAGKLPSISPALLRQLAGMGNNLNQIARQVNAA
GGTGHDRVQVMAALMAIDAGLERLRHAVLEKDAADDC
MLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPAKAGKLPSISPALLRQLAGMGNNLNQIARQVNAA
GGTGHDRVQVMAALMAIDAGLERLRHAVLEKDAADDC
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Host bacterium
| ID | 7560 | GenBank | NC_018985 |
| Plasmid name | pEAR5.2 | Incompatibility group | Col440II |
| Plasmid size | 5251 bp | Coordinate of oriT [Strand] | 2955..3014 [+] |
| Host baterium | Erwinia amylovora ATCC BAA-2158 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |