Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   107054
Name   oriT_pEN3-NDM in_silico
Organism   Enterobacter cloacae strain EN3
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MZ359668 (63173..63278 [+], 106 nt)
oriT length   106 nt
IRs (inverted repeats)      30..35, 41..46  (CCGCCG..CGGCGG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 106 nt

>oriT_pEN3-NDM
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   4664 GenBank   WP_015058937
Name   Relaxase_MCP57_RS00435_pEN3-NDM insolico UniProt ID   A0A7G3L766
Length   659 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 659 a.a.        Molecular weight: 75223.04 Da        Isoelectric Point: 9.9948

>WP_015058937.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETVLAVKSDMFEGLNNYLIRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQNTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNLPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(87-334)

Large polyvalent protein-associated domain 7 (LPD7)

(530-630)


  Protein structure


Source ID Structure
AlphaFold DB A0A7G3L766


T4CP

ID   4772 GenBank   WP_011091092
Name   t4cp2_MCP57_RS00095_pEN3-NDM insolico UniProt ID   _
Length   695 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 695 a.a.        Molecular weight: 79491.81 Da        Isoelectric Point: 4.8270

>WP_011091092.1 MULTISPECIES: hypothetical protein [Gammaproteobacteria]
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMHFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFADWLDGTDTDDVLYHQEDDLFPMIEM

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 66074..85357

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MCP57_RS00415 61159..61470 + 312 WP_015058934 hypothetical protein -
MCP57_RS00420 61603..62139 + 537 WP_015058935 hypothetical protein -
MCP57_RS00425 62640..63035 - 396 WP_015058936 hypothetical protein -
MCP57_RS00430 63034..63597 + 564 WP_227537722 plasmid mobilization protein MobA -
MCP57_RS00435 63584..65563 + 1980 WP_015058937 TraI/MobA(P) family conjugative relaxase -
MCP57_RS00440 65577..66077 + 501 WP_004187320 DotD/TraH family lipoprotein -
MCP57_RS00445 66074..66853 + 780 WP_015058938 type IV secretory system conjugative DNA transfer family protein traI
MCP57_RS00450 66864..68027 + 1164 WP_004187313 plasmid transfer ATPase TraJ virB11
MCP57_RS00455 68017..68277 + 261 WP_004187310 IcmT/TraK family protein traK
MCP57_RS00460 68302..71694 + 3393 WP_237737384 LPD7 domain-containing protein -
MCP57_RS00465 71618..72172 + 555 WP_015243643 hypothetical protein traL
MCP57_RS00470 72173..72370 - 198 WP_004187464 Hha/YmoA family nucleoid-associated regulatory protein -
MCP57_RS00475 72357..72767 - 411 WP_004187465 H-NS family nucleoid-associated regulatory protein -
MCP57_RS00480 72766..73548 + 783 WP_015058941 DotI/IcmL family type IV secretion protein traM
MCP57_RS00485 73557..74708 + 1152 WP_011091074 DotH/IcmK family type IV secretion protein traN
MCP57_RS00490 74720..76069 + 1350 WP_015058942 conjugal transfer protein TraO traO
MCP57_RS00495 76081..76785 + 705 WP_115431743 conjugal transfer protein TraP traP
MCP57_RS00500 76809..77339 + 531 WP_011091077 conjugal transfer protein TraQ traQ
MCP57_RS00505 77356..77745 + 390 WP_011091078 DUF6750 family protein traR
MCP57_RS00510 77791..78285 + 495 WP_015243645 hypothetical protein -
MCP57_RS00515 78282..81332 + 3051 WP_011091081 hypothetical protein traU
MCP57_RS00520 81329..82537 + 1209 WP_011091082 conjugal transfer protein TraW traW
MCP57_RS00525 82534..83184 + 651 WP_011091083 hypothetical protein -
MCP57_RS00530 83177..85357 + 2181 WP_004187492 DotA/TraY family protein traY
MCP57_RS00535 85360..86013 + 654 WP_011091084 hypothetical protein -
MCP57_RS00540 86070..86318 + 249 WP_015062836 IncL/M type plasmid replication protein RepC -

Host bacterium

ID   7490 GenBank   NZ_MZ359668
Plasmid name   pEN3-NDM Incompatibility group   IncL/M
Plasmid size   86615 bp Coordinate of oriT [Strand]   63173..63278 [+]
Host baterium   Enterobacter cloacae strain EN3

Cargo genes

Drug resistance gene   blaTEM-1B, armA, msr(E), mph(E), sul1, blaNDM-1
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -