Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   107052
Name   oriT_pMRY14-211ECL_2 in_silico
Organism   Enterobacter cloacae strain MRY14-211
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_AP018744 (37309..37409 [+], 101 nt)
oriT length   101 nt
IRs (inverted repeats)      80..85, 91..96  (AAAAAA..TTTTTT)
 20..26, 38..44  (TAAATCA..TGATTTA)
Location of nic site      62..63
Conserved sequence flanking the
  nic site    		  GGTGTATAGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 101 nt

>oriT_pMRY14-211ECL_2
TATTTATTTTTTTATCTTTTAAATCAGTATGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   4662 GenBank   WP_001749977
Name   mobF_IST05_RS00225_pMRY14-211ECL_2 insolico UniProt ID   A0A5C2CVP7
Length   1080 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1080 a.a.        Molecular weight: 120419.59 Da        Isoelectric Point: 6.5483

>WP_001749977.1 MULTISPECIES: MobF family relaxase [Gammaproteobacteria]
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATIADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKKEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKAGMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLSQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
SSGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEHEHEEGGHEI

  Protein domains


Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C_2)

(851-895)

TrwC relaxase (TrwC)

(16-287)

AAA domain (AAA_30)

(477-653)


  Protein structure


Source ID Structure
AlphaFold DB A0A5C2CVP7


Auxiliary protein

ID   2080 GenBank   WP_001749975
Name   WP_001749975_pMRY14-211ECL_2 insolico UniProt ID   A0A5C2CVS6
Length   138 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 138 a.a.        Molecular weight: 15332.60 Da        Isoelectric Point: 5.9670

>WP_001749975.1 MULTISPECIES: hypothetical protein [Gammaproteobacteria]
MPIITAKVSDELLAYIDLVSGGNRSDYLRRCIEAGPGDRESGLKIVADRLSDVNRKLDYLFDRASDADFG
PLRDELKAITETLSGVKFPPAGQMMLHESLAIETLILLRSIAEPGKTKAAKAEVERNGYKVWEPKKER

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A5C2CVS6


T4CP

ID   4769 GenBank   WP_001749976
Name   t4cp2_IST05_RS00230_pMRY14-211ECL_2 insolico UniProt ID   _
Length   509 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 509 a.a.        Molecular weight: 57679.73 Da        Isoelectric Point: 9.5788

>WP_001749976.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Gammaproteobacteria]
MDDRERGLAFLFAITLPPVMVWFLVAKFTYGIDPSTAKYLIPYLVKNTFSLWPLWSALIAGWFIGVGGLI
AFIIYDKSRVFKGERFKKIYRGTELVSARTLADKTRERGVNQLTVANIPIPTYAENLHFSIAGTTGTGKT
TIFNELLFKSIIRGGKNIALDPNGGFLKNFYRPGDVILNAYDKRTEGWVFFNEIRRSYDYERLVNSIVQE
SPDMATEEWFGYGRLIFSEVSKKLHSLYSTVTMEEVIHWACNVDQKKLKEFLMGTPAEAIFSGSEKAVGS
ARFVLSKNLAPHLKMPEGNFSLRDWLDDGKPGTLFITWQEEMKRSLNPLISCWLDSIFSIVLGMGEKESR
INVFIDELESLQFLPNLNDALTKGRKSGLCVYAGYQTYSQLVKVYGRDMAQTILANMRSNIVLGGSRLGD
ETLDQMSRSLGEIEGEVERKESDPQKPWIVRKRRDVKVVRAVTPTEISMLPNLTGYLALPGDMPVAKFKA
KHVKYHRKNPVPGIELRDI

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(113-489)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 16894..36742

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IST05_RS00085 12739..14172 - 1434 WP_001288432 DNA cytosine methyltransferase -
IST05_RS00090 14271..14543 + 273 Protein_17 IS1 family transposase -
IST05_RS00095 14554..14760 - 207 WP_001749967 hypothetical protein -
IST05_RS00100 14765..15412 - 648 WP_015344958 restriction endonuclease -
IST05_RS00105 15462..15695 - 234 WP_001191790 hypothetical protein -
IST05_RS00110 15809..16123 - 315 WP_001749965 TrbM/KikA/MpfK family conjugal transfer protein -
IST05_RS00115 16120..16464 - 345 WP_001749964 hypothetical protein -
IST05_RS00120 16480..16851 - 372 WP_011867773 H-NS family nucleoid-associated regulatory protein -
IST05_RS00125 16894..17628 + 735 WP_001749963 lytic transglycosylase domain-containing protein virB1
IST05_RS00130 17637..17918 + 282 WP_000440698 transcriptional repressor KorA -
IST05_RS00135 17928..18221 + 294 WP_001749962 hypothetical protein virB2
IST05_RS00140 18271..18588 + 318 WP_000496058 VirB3 family type IV secretion system protein virB3
IST05_RS00145 18588..21188 + 2601 WP_001749961 VirB4 family type IV secretion/conjugal transfer ATPase virb4
IST05_RS00150 21206..21919 + 714 WP_001749960 type IV secretion system protein virB5
IST05_RS00155 21927..22154 + 228 WP_001749959 IncN-type entry exclusion lipoprotein EexN -
IST05_RS00160 22170..23210 + 1041 WP_001749958 type IV secretion system protein virB6
IST05_RS00165 23281..23439 + 159 WP_012561180 hypothetical protein -
IST05_RS00170 23429..24127 + 699 WP_000646594 type IV secretion system protein virB8
IST05_RS00175 24138..25022 + 885 WP_000735066 TrbG/VirB9 family P-type conjugative transfer protein virB9
IST05_RS00180 25022..26182 + 1161 WP_000101710 type IV secretion system protein VirB10 virB10
IST05_RS00185 26224..27219 + 996 WP_000128596 ATPase, T2SS/T4P/T4SS family virB11
IST05_RS00190 27219..27752 + 534 WP_000792636 phospholipase D family protein -
IST05_RS00335 27926..28378 - 453 WP_001749956 DUF6710 family protein -
IST05_RS00200 28689..28931 + 243 WP_000844627 transposase -
IST05_RS00205 28963..29613 - 651 WP_000164043 tetracycline resistance transcriptional repressor TetR(A) -
IST05_RS00210 29719..30918 + 1200 WP_061349410 tetracycline efflux MFS transporter Tet(A) -
IST05_RS00215 30950..31603 - 654 Protein_42 EamA family transporter -
IST05_RS00220 31798..31971 - 174 WP_001749978 hypothetical protein -
IST05_RS00225 31971..35213 - 3243 WP_001749977 MobF family relaxase -
IST05_RS00230 35213..36742 - 1530 WP_001749976 type IV secretion system DNA-binding domain-containing protein virb4
IST05_RS00235 36744..37160 - 417 WP_001749975 hypothetical protein -
IST05_RS00340 37651..38070 + 420 WP_001749974 plasmid stabilization protein StbA -
IST05_RS00245 38079..38795 + 717 WP_001749973 StbB family protein -
IST05_RS00250 38797..39165 + 369 WP_000414913 plasmid stabilization protein StbC -
IST05_RS00255 39347..39691 + 345 WP_000999874 hypothetical protein -
IST05_RS00260 39802..40122 - 321 WP_001749971 protein CcgAII -
IST05_RS00265 40177..40356 - 180 WP_000214483 protein CcgAI -
IST05_RS00270 41083..41592 - 510 WP_000129958 antirestriction protein ArdA -

Host bacterium

ID   7488 GenBank   NZ_AP018744
Plasmid name   pMRY14-211ECL_2 Incompatibility group   IncN
Plasmid size   51983 bp Coordinate of oriT [Strand]   37309..37409 [+]
Host baterium   Enterobacter cloacae strain MRY14-211

Cargo genes

Drug resistance gene   blaIMP-1, aadA2, qacE, sul1, tet(A), blaCTX-M-2
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -