Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   107045
Name   oriT_pMUMC-2_4 in_silico
Organism   Enterobacter kobei strain MUMC-2
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP119977 (5536..5580 [+], 45 nt)
oriT length   45 nt
IRs (inverted repeats)      7..13, 25..31  (CGTTTCT..AGAAACG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 45 nt

>oriT_pMUMC-2_4
GCGCACCGTTTCTGAACGAAGTGAAGAAACGTCTAAGTGCGCCCT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   4654 GenBank   WP_040113349
Name   mobA_P2D90_RS00025_pMUMC-2_4 insolico UniProt ID   A0A734ICP3
Length   365 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 365 a.a.        Molecular weight: 42217.70 Da        Isoelectric Point: 6.3344

>WP_040113349.1 MULTISPECIES: mobilization protein MobA [Enterobacterales]
MASYHLSVKTGGKGSASPHADYISREGKYAREKDSDLEHKESGNMPAWAAHKPTEFWKAADTFERANGCT
YREIEIALPRELKPEQRLELVRDFVRQEIGDRHAYQFAIHNPKAAIAGGEQPHAHIMFSERMNDGIDRDP
QQYFKRANSKAPERGGAKKARFGETPTERKEHLVAQRERWADLQNKHLERYQHTDRVDARSLKAQGIERE
PERHLGAGQVQRFDTAQLQAILERRDAERQAEQSRDERDSVIDVTTSLREAISERDNLTIKQEQKPEPAQ
EDTSGRVFDFEKEPEKLNALVSDALKDVQDEIDLQSLVNETMAEFQGIHQEMERLAEKQRQQEKEQQRLA
EQTRQKPDKGWSFSR

  Protein domains


Predicted by InterproScan.

MobA/MobL family (MobA_MobL)

(42-218)


  Protein structure


Source ID Structure
AlphaFold DB A0A734ICP3


Host bacterium

ID   7481 GenBank   NZ_CP119977
Plasmid name   pMUMC-2_4 Incompatibility group   ColE10
Plasmid size   12808 bp Coordinate of oriT [Strand]   5536..5580 [+]
Host baterium   Enterobacter kobei strain MUMC-2

Cargo genes

Drug resistance gene   mcr-4.3
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -