Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   107041
Name   oriT_ENCL58|I in_silico
Organism   Enterobacter cloacae isolate Enterobacter cloacae ENCL58
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_LT882699 (168790..168940 [-], 151 nt)
oriT length   151 nt
IRs (inverted repeats)      93..98, 106..111  (TGGCCT..AGGCCA)
 12..17, 24..29  (AACCCT..AGGGTT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 151 nt

>oriT_ENCL58|I
TGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   4650 GenBank   WP_001337757
Name   mobH_GWA23_RS01075_ENCL58|I insolico UniProt ID   A0A709FRI2
Length   992 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 992 a.a.        Molecular weight: 109581.85 Da        Isoelectric Point: 4.8956

>WP_001337757.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIETAPSARVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEADDGDDQEEGEAALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGPTESSK
PDAGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPF
DAFNASAETTSTDATNSEIPDVAMPGKQEEQPKQDFVPQEQNSLQGDDFPMFGGSDEPPSWAIEPLPMLT
DAPEQPTHTPEMPHTDNVNQHEKDAKTLLVEMLSGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVI
LYPDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQ
DAFELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKASPEQKAKGKDSQPQPKEKKVDVTSPVEEQQ
RKPVQEKQNVARLPKREAQPVAPEPKVEREKELGHVEVREREDPEVREFEPPKAKTNPKDINAEDFLPSG
VTPQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKK
RQGKLYLEVNET

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure


Source ID Structure
AlphaFold DB A0A709FRI2


T4CP

ID   4751 GenBank   WP_000637386
Name   traC_GWA23_RS00995_ENCL58|I insolico UniProt ID   _
Length   815 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 815 a.a.        Molecular weight: 92590.87 Da        Isoelectric Point: 5.9222

>WP_000637386.1 MULTISPECIES: type IV secretion system protein TraC [Gammaproteobacteria]
MIVTIKKKLEETLIPEHLRAAGIIPVLAYDEDDHVFLMDDHSAGFGFMCEPLCGADEKVQERMNGFLNQE
FPSKTTLQFVLFRSPDINQEMYRMMGLRDGFRHELLTSVIKERINFLQHHTTERIFAKTNKGIYDNGLIQ
DLKLFVTCKVPIKNNNPTESELQQLAQLRTKVESSLQTVGLRPRTMTAVNYIRIMSTILNWGPDASWRHD
SVDWEMDKPICEQIFDYGTDVEVSKNGIRLGDYHAKVMSAKKLPDVFYFGDALTYAGDLSGGNSSIKENY
MVVTNVFFPEAESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTLYESMKEGAKPVKITYSVVLF
APTKERVEAAAMAARNIWRESRFELMEDKFVALPMFLNCLPFCTDRDAVRDLFRYKTMTTEQAAVVLPVF
GEWKGTGTYHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELIFSYLSEGAQVWVIDAGKS
YQKLSEMLNGDFVHFEEGTHVCLNPFELIQNYEDEEDAIVSLVCAMASAKGLLDEWQISALKQVLSRLWE
EKGKEMKVDDIAERCLEEENDQRLKDIGQQLYAFTSKGSYGKYFSRKNNVSFQNQFTVLELDELQGRKHL
RQVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSVFMEHAYRKFRKYGGSVVIATQSINDLY
ENAVGRAIAENSASMYLLGQTEETVESVKRSGRLTLSEGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRL
IVGDFQKLLYSTDPVDVNAIDQFVKQGMSIPEAIKAVMRSRQQAA

  Protein domains


Predicted by InterproScan.

TraG P-loop domain (P-loop_TraG)

(449-809)

Family of unknown function (DUF5934) (DUF5934)

(284-434)

TraC protein (TraC_F_IV)

(21-255)

  Protein structure



No available structure.



ID   4752 GenBank   WP_000178856
Name   traD_GWA23_RS01070_ENCL58|I insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 69197.66 Da        Isoelectric Point: 6.9403

>WP_000178856.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTMSYDPLAYEMPWRPNYEKNAVAGWLAASGAALAVEQVSTMPPEPFYWMTGICGVMAMARLPKAIKLHL
LQKHLKGRDLEFISIAELQKYIKDTPDDMWLGSGFLWENRHAQRVFEILKRDWTSIVGRESTVKKVVRKI
QGKKKELPIGQPWIHGVEPKEEKLMQPLKHTEGHSLIVGTTGSGKTRMFDILISQAILRGEAVIIIDPKG
DKEMRDNARRACEAMGQPERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEAGADPFKSFGWQ
ALNNIAQGLVITHDRPNLTKLRRFLEGGAAGLVIKAVQAYSERVMPDWEAEAAAYLEKAKNGSREKIAFA
LMKFYYDIIQPEHPNSDLEGLLSMFQHDQTHFSKMVANLLPIMNMLTSGELGPLLSPDSSDLSDERQITD
SAKIINNAQVAYLGLDSLTDNMVGSAMGSIFLSDLTAVAGDRYNYGVNNRPVNIFVDEAAEVINDPFIQL
LNKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNTFALRIVDGETQEYIADNLPKTRLKYVMRT
QGQNSDGKEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGTIVKGRLPILTQ

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(471-598)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(170-297)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 145665..170856

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GWA23_RS00935 (PENCL58_190) 140792..141700 - 909 WP_000739139 hypothetical protein -
GWA23_RS00940 (PENCL58_191) 141711..142679 - 969 WP_000085162 AAA family ATPase -
GWA23_RS00945 (PENCL58_193) 142899..143084 - 186 WP_001186917 hypothetical protein -
GWA23_RS00950 (PENCL58_194) 143388..143708 - 321 WP_000547566 hypothetical protein -
GWA23_RS00955 (PENCL58_195) 144002..144643 + 642 WP_000796664 hypothetical protein -
GWA23_RS00960 (PENCL58_196) 144766..145626 + 861 WP_000709517 hypothetical protein -
GWA23_RS00965 (PENCL58_197) 145665..148463 - 2799 WP_001256487 conjugal transfer mating pair stabilization protein TraN traN
GWA23_RS00970 (PENCL58_198) 148579..149583 - 1005 WP_043940352 TraU family protein traU
GWA23_RS00975 (PENCL58_199) 149583..150254 - 672 WP_001337754 EAL domain-containing protein -
GWA23_RS00980 (PENCL58_200) 150251..151516 - 1266 WP_000621288 TrbC family F-type conjugative pilus assembly protein traW
GWA23_RS00985 (PENCL58_201) 151479..152009 - 531 WP_001010738 S26 family signal peptidase -
GWA23_RS00990 (PENCL58_202) 152006..152323 - 318 WP_000351984 hypothetical protein -
GWA23_RS00995 (PENCL58_203) 152338..154785 - 2448 WP_000637386 type IV secretion system protein TraC virb4
GWA23_RS01000 (PENCL58_204) 154782..155489 - 708 WP_001259347 DsbC family protein -
GWA23_RS01005 (PENCL58_205) 155638..161169 - 5532 WP_000606833 Ig-like domain-containing protein -
GWA23_RS01010 (PENCL58_206) 161370..161762 - 393 WP_000479535 TraA family conjugative transfer protein -
GWA23_RS01015 (PENCL58_207) 161766..162344 - 579 WP_000793435 type IV conjugative transfer system lipoprotein TraV traV
GWA23_RS01020 (PENCL58_208) 162341..163654 - 1314 WP_024131605 TraB/VirB10 family protein traB
GWA23_RS01025 (PENCL58_209) 163654..164571 - 918 WP_000794249 type-F conjugative transfer system secretin TraK traK
GWA23_RS01030 (PENCL58_210) 164555..165181 - 627 WP_001049716 TraE/TraK family type IV conjugative transfer system protein traE
GWA23_RS01035 (PENCL58_211) 165178..165459 - 282 WP_000805625 type IV conjugative transfer system protein TraL traL
GWA23_RS01040 (PENCL58_212) 165604..165969 - 366 WP_001052531 hypothetical protein -
GWA23_RS01215 (PENCL58_213) 165981..166124 - 144 WP_001275801 hypothetical protein -
GWA23_RS01045 (PENCL58_214) 166305..166967 - 663 WP_001231463 hypothetical protein -
GWA23_RS01050 (PENCL58_215) 166967..167344 - 378 WP_000869261 hypothetical protein -
GWA23_RS01055 167354..167800 - 447 WP_000122506 hypothetical protein -
GWA23_RS01060 (PENCL58_217) 167810..168439 - 630 WP_000743450 DUF4400 domain-containing protein tfc7
GWA23_RS01065 (PENCL58_218) 168396..168980 - 585 WP_000332866 hypothetical protein -
GWA23_RS01070 (PENCL58_219) 168991..170856 - 1866 WP_000178856 conjugative transfer system coupling protein TraD virb4
GWA23_RS01075 (PENCL58_220) 170853..173831 - 2979 WP_001337757 MobH family relaxase -
GWA23_RS01080 (PENCL58_221) 173999..174616 + 618 WP_001249396 hypothetical protein -
GWA23_RS01085 (PENCL58_222) 174598..174831 + 234 WP_001191892 hypothetical protein -

Host bacterium

ID   7477 GenBank   NZ_LT882699
Plasmid name   ENCL58|I Incompatibility group   IncA/C2
Plasmid size   177037 bp Coordinate of oriT [Strand]   168790..168940 [-]
Host baterium   Enterobacter cloacae isolate Enterobacter cloacae ENCL58

Cargo genes

Drug resistance gene   sul2, blaOXA-427, blaTEM-1B, aph(6)-Id, aph(3'')-Ib
Virulence gene   -
Metal resistance gene   merR, merT, merP, merA, merD, merE, merC
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -