Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 107037 |
| Name | oriT_pA22732-IMP  |
| Organism | Achromobacter xylosoxidans strain A22732 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_KJ588780 (33586..33684 [+], 99 nt) |
| oriT length | 99 nt |
| IRs (inverted repeats) | 24..31, 34..41 (TAACCGGC..GCCGGTTA) |
| Location of nic site | 60..61 |
| Conserved sequence flanking the nic site |
TCCTGCCCGC |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 99 nt
>oriT_pA22732-IMP
GAATAAGGGACAGTGAAGATAGATAACCGGCTCGCCGGTTAGCTAACTTCACACATCCTGCCCGCCTTACGGCGTTAATAACACCAAGGAAAGTCTACA
GAATAAGGGACAGTGAAGATAGATAACCGGCTCGCCGGTTAGCTAACTTCACACATCCTGCCCGCCTTACGGCGTTAATAACACCAAGGAAAGTCTACA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 4746 | GenBank | WP_011114047 |
| Name | t4cp2_HTC88_RS00035_pA22732-IMP |
UniProt ID | _ |
| Length | 852 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 852 a.a. Molecular weight: 93980.15 Da Isoelectric Point: 6.3901
>WP_011114047.1 MULTISPECIES: VirB4 family type IV secretion/conjugal transfer ATPase [Bacteria]
MIEAIAIAIAVLGAVLLLILFARIRAVDAELKLKKHRAKDAGLADLLNYAAVVDDGVIVGKNGSFMAAWL
YKGDDNASSTEEQREMVSFRINQALAGLGNGWMVHVDAVRRPAPNYSERGVSSFPDSVSFAIDEERRRLF
EGLGAMFEGYFVLTVTWFPPVLAQRKFVELMFDDDAVAPDHTARTTGLIAQFKREITSLESRLSSAVKMT
RLRGQKVVSEEGREVTHDDFLSWLQFCVTGLHHPVMLPSNPMYLDAVIGGQELWGGVVPKIGRKFIQVVA
IEGFPLESTPGVLSALAELPSEYRWSSRFIFMDQHESLKHLDKFRKKWKQKIRGFFDQVFNTNTGSINQD
AAAMVGDAEAAIAEVNSGLVAAGYYTSVVVLMDEDRERLAASALLVEKAVNRLAFAARIETINTLDAYLG
SLPGHGVENVRRPLINTMNLADLLPTSSIWTGSATAPCPMYPPLSPALMHCVTVGATPFRLNLHVRDLGH
TFMFGPTGAGKSTHLGIIAAQLRRYAGMSIYAFDKGMSMYPLAAGIRAATKGKSGLHFTVAADDDRLAFC
PLQFLETKGDRAWAMEWIDTILALNGVNTTPAQRNEIGNAIMSMHASGARTLSEFSVTIQDEAIREAIKQ
YTVDGSMGHLLDAEEDGLSLSDFTVFEIEELMNLGEKFALPVLLYLFRRIERALKGQPSVIILDEAWLML
GHPAFRAKIREWLKVLRKANCLVLMATQSLSDAANSGILDVIVESTATKIFLPNVYARDEDTSALYRRMG
LNARQIEILATAIPKRQYYYVSENGRRLYDLALGPMALAFVGASDKESVATIKSLEAKFGHEWVHEWLAG
RGLNLNDYLEAA
MIEAIAIAIAVLGAVLLLILFARIRAVDAELKLKKHRAKDAGLADLLNYAAVVDDGVIVGKNGSFMAAWL
YKGDDNASSTEEQREMVSFRINQALAGLGNGWMVHVDAVRRPAPNYSERGVSSFPDSVSFAIDEERRRLF
EGLGAMFEGYFVLTVTWFPPVLAQRKFVELMFDDDAVAPDHTARTTGLIAQFKREITSLESRLSSAVKMT
RLRGQKVVSEEGREVTHDDFLSWLQFCVTGLHHPVMLPSNPMYLDAVIGGQELWGGVVPKIGRKFIQVVA
IEGFPLESTPGVLSALAELPSEYRWSSRFIFMDQHESLKHLDKFRKKWKQKIRGFFDQVFNTNTGSINQD
AAAMVGDAEAAIAEVNSGLVAAGYYTSVVVLMDEDRERLAASALLVEKAVNRLAFAARIETINTLDAYLG
SLPGHGVENVRRPLINTMNLADLLPTSSIWTGSATAPCPMYPPLSPALMHCVTVGATPFRLNLHVRDLGH
TFMFGPTGAGKSTHLGIIAAQLRRYAGMSIYAFDKGMSMYPLAAGIRAATKGKSGLHFTVAADDDRLAFC
PLQFLETKGDRAWAMEWIDTILALNGVNTTPAQRNEIGNAIMSMHASGARTLSEFSVTIQDEAIREAIKQ
YTVDGSMGHLLDAEEDGLSLSDFTVFEIEELMNLGEKFALPVLLYLFRRIERALKGQPSVIILDEAWLML
GHPAFRAKIREWLKVLRKANCLVLMATQSLSDAANSGILDVIVESTATKIFLPNVYARDEDTSALYRRMG
LNARQIEILATAIPKRQYYYVSENGRRLYDLALGPMALAFVGASDKESVATIKSLEAKFGHEWVHEWLAG
RGLNLNDYLEAA
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 4747 | GenBank | WP_019373230 |
| Name | t4cp2_HTC88_RS00160_pA22732-IMP |
UniProt ID | _ |
| Length | 637 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 637 a.a. Molecular weight: 69925.10 Da Isoelectric Point: 8.2999
>WP_019373230.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Pseudomonadota]
MKIKMNNAVGPQVRTAKPKPSKLLPVLGAASMVGGLQAATQFFAHTFAYHATLGPNVGHVYAPWSILHWT
YKWYSQYPDEIMKAGSMGMLVSTVGLLGVAVARVVTSNSSKANEYLHGSARWAEKKDIQAAGLLPRERNV
LEIVTGKAAPTATGVYVGGWQDKDGNFFYLRHSGPEHVLTYAPTRSGKGVGLVVPTLLSWGASSVITDLK
GELWALTAGWRQKHAKNKVLRFEPASTSGGVCWNPLDEIRLGTEYEVGDVQNLATLIVDPDGKGLDSHWQ
KTAFALLVGVILHALYKAKDDGGTATLPSVDAMLADPNRDIGELWMEMATYGHVDGQNHHAIGSAARDMM
DRPEEEAGSVLSTAKSYLALYRDPVVARNVSRSDFRIKQLMHEDDPVSLYIVTQPNDKARLRPLVRVMVN
MIVRLLADKMDFEGGRPVAHYKHRLLMMLDEFPSLGKLEIMQESLAFVAGYGIKCYLICQDINQLKSRET
GYGHDESITSNCHVQNAYPPNRVETAEHLSRLTGQTTVVKEQITTSGRRTAAMLGQVSRTYQEVQRPLLT
PDECLRMPGPKKNAQGEIEEAGDMVIYVAGYPAIYGKQPLYFKDPVFSARAAIPAPKVSDRLRAVAQAET
EGEGITI
MKIKMNNAVGPQVRTAKPKPSKLLPVLGAASMVGGLQAATQFFAHTFAYHATLGPNVGHVYAPWSILHWT
YKWYSQYPDEIMKAGSMGMLVSTVGLLGVAVARVVTSNSSKANEYLHGSARWAEKKDIQAAGLLPRERNV
LEIVTGKAAPTATGVYVGGWQDKDGNFFYLRHSGPEHVLTYAPTRSGKGVGLVVPTLLSWGASSVITDLK
GELWALTAGWRQKHAKNKVLRFEPASTSGGVCWNPLDEIRLGTEYEVGDVQNLATLIVDPDGKGLDSHWQ
KTAFALLVGVILHALYKAKDDGGTATLPSVDAMLADPNRDIGELWMEMATYGHVDGQNHHAIGSAARDMM
DRPEEEAGSVLSTAKSYLALYRDPVVARNVSRSDFRIKQLMHEDDPVSLYIVTQPNDKARLRPLVRVMVN
MIVRLLADKMDFEGGRPVAHYKHRLLMMLDEFPSLGKLEIMQESLAFVAGYGIKCYLICQDINQLKSRET
GYGHDESITSNCHVQNAYPPNRVETAEHLSRLTGQTTVVKEQITTSGRRTAAMLGQVSRTYQEVQRPLLT
PDECLRMPGPKKNAQGEIEEAGDMVIYVAGYPAIYGKQPLYFKDPVFSARAAIPAPKVSDRLRAVAQAET
EGEGITI
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 2395..13072
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HTC88_RS00005 (pA22732_00001) | 1..1221 | - | 1221 | WP_011114072 | plasmid replication initiator TrfA | - |
| HTC88_RS00010 (pA22732_00002) | 1268..1609 | - | 342 | WP_000019167 | single-stranded DNA-binding protein | - |
| HTC88_RS00015 (pA22732_00003) | 1723..2085 | + | 363 | WP_010890126 | transcriptional regulator | - |
| HTC88_RS00020 (pA22732_00004) | 2395..3357 | + | 963 | WP_000132020 | P-type conjugative transfer ATPase TrbB | virB11 |
| HTC88_RS00025 (pA22732_00005) | 3374..3838 | + | 465 | WP_011114046 | conjugal transfer system pilin TrbC | virB2 |
| HTC88_RS00030 (pA22732_00006) | 3842..4153 | + | 312 | WP_001207961 | conjugal transfer protein TrbD | virB3 |
| HTC88_RS00035 (pA22732_00007) | 4150..6708 | + | 2559 | WP_011114047 | VirB4 family type IV secretion/conjugal transfer ATPase | virb4 |
| HTC88_RS00040 (pA22732_00008) | 6705..7487 | + | 783 | WP_006122481 | conjugal transfer protein TrbF | virB8 |
| HTC88_RS00045 (pA22732_00009) | 7505..8404 | + | 900 | WP_006122480 | P-type conjugative transfer protein TrbG | virB9 |
| HTC88_RS00050 (pA22732_00010) | 8407..8895 | + | 489 | WP_006122479 | conjugal transfer protein TrbH | - |
| HTC88_RS00055 (pA22732_00011) | 8900..10321 | + | 1422 | WP_011114050 | TrbI/VirB10 family protein | virB10 |
| HTC88_RS00060 (pA22732_00012) | 10342..11106 | + | 765 | WP_010890135 | P-type conjugative transfer protein TrbJ | virB5 |
| HTC88_RS00065 (pA22732_00013) | 11116..11343 | + | 228 | WP_000644148 | entry exclusion lipoprotein TrbK | - |
| HTC88_RS00070 (pA22732_00014) | 11354..13072 | + | 1719 | WP_027814021 | P-type conjugative transfer protein TrbL | virB6 |
| HTC88_RS00075 (pA22732_00015) | 13090..13677 | + | 588 | WP_010890138 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| HTC88_RS00080 (pA22732_00016) | 13691..14326 | + | 636 | WP_011114052 | transglycosylase SLT domain-containing protein | - |
| HTC88_RS00085 (pA22732_00017) | 14355..14621 | + | 267 | WP_172685950 | conjugal transfer protein TrbO | - |
| HTC88_RS00090 (pA22732_00018) | 14621..15319 | + | 699 | WP_011114053 | TraX family protein | - |
| HTC88_RS00095 (pA22732_00019) | 15335..15766 | + | 432 | WP_001665065 | OmpA family protein | - |
| HTC88_RS00100 (pA22732_00020) | 15921..16595 | + | 675 | WP_011178411 | DNA methylase | - |
| HTC88_RS00105 (pA22732_00021) | 16597..17448 | - | 852 | WP_011296189 | recombinase family protein | - |
| HTC88_RS00110 | 17491..17562 | + | 72 | Protein_21 | EAL domain-containing protein | - |
| HTC88_RS00115 (pA22732_00022) | 17637..18065 | + | 429 | Protein_22 | helix-turn-helix domain-containing protein | - |
Host bacterium
| ID | 7473 | GenBank | NZ_KJ588780 |
| Plasmid name | pA22732-IMP | Incompatibility group | IncP1 |
| Plasmid size | 49804 bp | Coordinate of oriT [Strand] | 33586..33684 [+] |
| Host baterium | Achromobacter xylosoxidans strain A22732 |
Cargo genes
| Drug resistance gene | blaIMP-1 |
| Virulence gene | - |
| Metal resistance gene | merR, merT, merP, merA, merD, merE |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |