Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   107036
Name   oriT1_pHS08-175-2-52445bp-repAIncN in_silico
Organism   Enterobacter hormaechei subsp. steigerwaltii strain 08-175
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MT010563 (9295..9354 [+], 60 nt)
oriT length   60 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 60 nt

>oriT1_pHS08-175-2-52445bp-repAIncN
GGGTGTCGGGGCGCAGCCCTGACCCAGTCACATAGCGATAGCGGAGTGTATACTGGCTTA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   4646 GenBank   WP_000884351
Name   mobF_MJ822_RS00255_pHS08-175-2-52445bp-repAIncN insolico UniProt ID   D7RVP6
Length   1076 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1076 a.a.        Molecular weight: 119897.16 Da        Isoelectric Point: 6.6071

>WP_000884351.1 MULTISPECIES: MobF family relaxase [Enterobacterales]
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATIADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKKEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKAGMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
ISGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEEGGHEI

  Protein domains


Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C_2)

(851-895)

TrwC relaxase (TrwC)

(16-287)

AAA domain (AAA_30)

(477-653)


  Protein structure


Source ID Structure
AlphaFold DB D7RVP6


Auxiliary protein

ID   2075 GenBank   WP_013188476
Name   WP_013188476_pHS08-175-2-52445bp-repAIncN insolico UniProt ID   D7RVK1
Length   138 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 138 a.a.        Molecular weight: 15332.60 Da        Isoelectric Point: 5.9670

>WP_013188476.1 MULTISPECIES: hypothetical protein [Enterobacterales]
MPIITAKVSDELLAYIDLVSGGNRSDYLRRCLEAGPGDRESGLKIVADRLSDVNRKLDYIFDRASDADFG
PLRDELKAITETLSGVKFPPAGQMMLHESLAIETLILLRSIAEPGKTKAAKAEVERNGYKVWEPKKER

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB D7RVK1


T4CP

ID   4745 GenBank   WP_000342688
Name   t4cp2_MJ822_RS00260_pHS08-175-2-52445bp-repAIncN insolico UniProt ID   _
Length   509 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 509 a.a.        Molecular weight: 57762.87 Da        Isoelectric Point: 9.6551

>WP_000342688.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MDDRERGLAFLFAITLPPVMVWFLVAKFTYGIDPSTAKYLIPYLVKNTFSLWPLWSALIAGWFIGVGGLI
AFIIYDKSRVFKGERFKKIYRGTELVRARTLADKTRERGVNQLTVANIPIPTYAENLHFSIAGTTGTGKT
TIFNELLFKSIIRGGKNIALDPNGGFLKNFYRPGDVILNAYDKRTEGWVFFNEIRRSYDYERLVNSIVQE
SPDMATEEWFGYGRLIFSEVSKKLHSLYSTVTMEEVIHWACNVDQKKLKEFLMGTPAEAIFSGSEKAVGS
ARFVLSKNLAPHLKMPEGNFSLRDWLDDGKPGTLFITWQEEMKRSLNPLISCWLDSIFSIVLGMGEKESR
INVFIDELESLQFLPNLNDALTKGRKSGLCVYAGYQTYSQLVKVYGRDMAQTILANMRSNIVLGGSRLGD
ETLDQMSRSLGEIEGEVERKESDPQKPWIVRKRRDVKVVRAVTPTEISMLPNLTGYLALPGDMPVAKFKA
KHVKYHRKNPVPGIELREI

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(113-489)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 12976..22264

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ822_RS00355 7985..8683 - 699 Protein_9 relaxase/mobilization nuclease domain-containing protein -
MJ822_RS00055 8625..8948 - 324 WP_040090312 MobC family plasmid mobilization relaxosome protein -
MJ822_RS00060 9013..9204 + 192 WP_000165985 Rop family plasmid primer RNA-binding protein -
MJ822_RS00065 9874..9987 + 114 WP_071699851 replication initiation protein -
MJ822_RS00070 10205..10345 - 141 WP_072020761 colicin R system lysis protein -
MJ822_RS00075 10465..10989 + 525 WP_040090309 colicin R immunity protein -
MJ822_RS00080 11017..11838 - 822 Protein_15 pore-forming bacteriocin colicin R -
MJ822_RS00085 11882..12586 - 705 WP_001067855 IS6-like element IS26 family transposase -
MJ822_RS00090 12650..12934 - 285 Protein_17 conjugal transfer protein TraG -
MJ822_RS00095 12976..14136 - 1161 WP_000101712 type IV secretion system protein VirB10 virB10
MJ822_RS00100 14136..15020 - 885 WP_000735067 TrbG/VirB9 family P-type conjugative transfer protein virB9
MJ822_RS00105 15031..15729 - 699 WP_000646594 type IV secretion system protein virB8
MJ822_RS00110 15719..15877 - 159 WP_012561180 hypothetical protein -
MJ822_RS00115 15948..16988 - 1041 WP_000886022 type IV secretion system protein virB6
MJ822_RS00120 17004..17231 - 228 WP_000734973 IncN-type entry exclusion lipoprotein EexN -
MJ822_RS00125 17239..17952 - 714 WP_000749362 type IV secretion system protein virB5
MJ822_RS00130 17970..20570 - 2601 WP_001200711 VirB4 family type IV secretion/conjugal transfer ATPase virb4
MJ822_RS00135 20570..20887 - 318 WP_000496058 VirB3 family type IV secretion system protein virB3
MJ822_RS00140 20937..21230 - 294 WP_000209137 TrbC/VirB2 family protein virB2
MJ822_RS00145 21240..21521 - 282 WP_000440698 transcriptional repressor KorA -
MJ822_RS00150 21530..22264 - 735 WP_000033783 lytic transglycosylase domain-containing protein virB1
MJ822_RS00155 22307..22678 + 372 WP_011867773 H-NS family nucleoid-associated regulatory protein -
MJ822_RS00160 22694..23071 + 378 WP_000504250 hypothetical protein -
MJ822_RS00165 23034..23348 + 315 WP_001749965 TrbM/KikA/MpfK family conjugal transfer protein -
MJ822_RS00170 23462..23695 + 234 WP_001191790 hypothetical protein -
MJ822_RS00175 23745..24392 + 648 WP_001532074 restriction endonuclease -
MJ822_RS00180 24397..24594 + 198 WP_013188474 hypothetical protein -
MJ822_RS00185 24614..24885 - 272 Protein_36 IS1 family transposase -
MJ822_RS00190 24979..26412 + 1434 WP_001288433 DNA cytosine methyltransferase -

Host bacterium

ID   7472 GenBank   NZ_MT010563
Plasmid name   pHS08-175-2-52445bp-repAIncN Incompatibility group   IncN
Plasmid size   52445 bp Coordinate of oriT [Strand]   9295..9354 [+]; 40830..40930 [+]
Host baterium   Enterobacter hormaechei subsp. steigerwaltii strain 08-175

Cargo genes

Drug resistance gene   fosA3, blaCTX-M-125
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -